Recombinant Mouse RGM-B Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse RGM-B Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immoblized rhBMP-4 at 1 µg/mL (100 µL/well) can bind rmRGM-B with a linear range of 0.016‑1 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse RGM-B protein
  Gly49-Asp171 (N-terminus chain) & Pro172-Ser414 (C-terminus chain), with a C-terminal 6-His tag
• Accession #: Q7TQ33
• N-terminal Sequence: Gly49 (N-terminus chain) & Pro172 (C-terminus chain)
• Protein/Peptide Type: Recombinant Proteins
• Gene: Rgmb
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 13.3 kDa (N-terminus chain), 27.8 kDa (C-terminus chain).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 20 kDa and 27 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse RGM-B Protein, CF

• DKFZp434P228
• Dragon
• DRG11-responsive axonal guidance and outgrowth of neurite
• RGM domain family, member B
• RGMB
• RGM-B

Background

RGM-B, also known as DRAGON, is a 40 kDa member of the repulsive guidance molecule (RGM) family of GPI-linked neuronal and muscle membrane proteins (1, 2). It is synthesized as a preproprotein that consists of a 48 amino acid (aa) signal sequence, a 367 aa mature region, and a 21 aa C-terminal prosegment (3). RGM-B contains two potential N-linked glycosylation sites and an abbreviated von Willebrand factor domain. Potential proteolytic cleavage within the VWF domain is supported by R&D Systems’ in house data (4). Within the region following the VWF domain, mouse RGM-B shares 49% and 43% aa sequence identity with RGM-A and RGM-C, respectively. It shares 90%, 79%, 92%, and 93% aa sequence identity with bovine, chicken, human, and macaque RGM-B, respectively. RGM-B is expressed in the developing and adult nervous system, particularly in the dorsal root ganglia and mantle layer of the spinal cord (3 - 5). In mouse, it shows a complementary, non-overlapping distribution with RGM-A (2 - 5). RGM-B is also expressed in fetal and adult enteric ganglia and in postnatal intestinal epithelium (6). RGM-B expression has been detected in neuronal cell bodies and proximal axonal segments (4) but is also present on the cell surface, where it interacts homophilically and mediates neuronal adhesion (3). RGM-B additionally functions as a BMP coreceptor. It directly binds BMP-2 and -4 but not other TGF-beta family proteins (7). RGM-B associates with BMP type I (ALK-2, -3, -6) and type II (Activin RIIA, Activin RIIB) receptors and enhances BMP signaling (7).

1. Monnier, P.P. et al. (2002) Nature 419:392.
2. Schmidtmer, J. and D. Engelkamp (2004) Gene Exp. Patterns 4:105.
3. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
4. Niederkofler, V. et al. (2004) J. Neurosci. 24:808.
5. Oldekamp, J. et al. (2004) Gene Exp. Patterns 4:283.
6. Metzger, M. et al. (2005) Dev. Dyn. 234:169.
7. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.

Publications for RGM-B (3597-RG)(3)

Publications using 3597-RG

• X Nie, W Chen, Y Zhu, B Huang, W Yu, Z Wu, S Guo, Y Zhu, L Luo, S Wang, L Chen B7-DC (PD-L2) costimulation of CD4(+) T-helper 1 response via RGMb Cell. Mol. Immunol., 2017-05-08;0(0):. 2017-05-08 [PMID: 28479601] (Surface Plasmon Resonance, Mouse)
• Xiao , Yanping, Yu , Sanhong, Zhu , Baogong, Bedoret , Denis, Bu , Xia, Francisco , Loise M, Hua , Ping, Duke-Cohan , Jonathan, Umetsu , Dale T, Sharpe , Arlene H, DeKruyff , Rosemari, Freeman , Gordon J RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance. J Exp Med, 2014-04-21;211(5):943-59. 2014-04-21 [PMID: 24752301] (ELISA (Capture), In Vivo, Mouse, Rat)
• Liu X, Hashimoto M, Horii H, Yamaguchi A, Naito K, Yamashita T Repulsive guidance molecule b inhibits neurite growth and is increased after spinal cord injury. Biochem. Biophys. Res. Commun., 2009-03-24;382(4):795-800. 2009-03-24 [PMID: 19324014] (Bioassay, Rat)

Bioinformatics

• Gene Symbol: Rgmb
• Uniprot:
  • Q7TQ33()