Recombinant Mouse Ret Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse Ret Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Serial dilution of immobilized rmRet (100 µL/well) was tested for binding with the GDNF-GFR alpha -1 complex using 4 ng/mL rhGDNF and 1 µg/mL rrGFR alpha -1/Fc Chimera. The concentration of immobilized Ret that produces 50% of the optimal binding response is found to be approximately 2-5 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Ret protein
  

  Mouse cRet Leu29 - Arg637 (Phe174Ser) Accession # P35546.2	IEGRMD	Human IgG1 (Pro100 - Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #: P35546.2
• N-terminal Sequence: Leu29
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ret
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 95 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 150 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Ret Fc Chimera Protein, CF

• CDHF12MTC1
• CDHR16
• EC 2.7.10
• Hirschsprung disease 1
• Hirschsprung disease)
• MEN2A
• MEN2B
• multiple endocrine neoplasia and medullary thyroid carcinoma 1
• ret proto-oncogene
• RET transforming sequence
• Ret

Background

The GDNF family of neurotrophic factors consititute a new family of factors within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and in the adult animal. The GDNF family members (GDNF, neurturin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha -1 -4). GFR alpha -1, -2, and -4 are the preferred ligand-binding subunits for GDNF, neurturin and persephin, respectively. To date, the preferred ligand for GFR alpha -3 has not been identified. The Ret tyrosine-kinase receptor is encoded by the c-ret proto-oncogene. Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Mouse Ret cDNA encodes a 1115 amino acid (aa) residue transmembrane tyrosine kinase with a 28 aa residue signal peptide, a 609 aa residue cysteine-rich extracellular domain and a 456 aa residue cytoplasmic domain. A cadherin-related sequence is also present in the extracellular domain of Ret. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha  complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha .

1. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
2. Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
3. Carlomagno, F. et al. (1998) Endocrinology, 139:3613.

Publications for Ret (482-RT/CF)(5)

Publications using 482-RT/CF

• Vaegter CB, Jansen P, Fjorback AW Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling. Nat. Neurosci., 2010-11-21;14(1):54-61. 2010-11-21 [PMID: 21102451] (Surface Plasmon Resonance)
• Li J, Klein C, Liang C, Rauch R, Kawamura K, Hsueh AJ Autocrine regulation of early embryonic development by the artemin-GFRA3 (GDNF family receptor-alpha 3) signaling system in mice. FEBS Lett., 2009-07-04;583(15):2479-85. 2009-07-04 [PMID: 19580811] (Bioassay, Mouse)
• Lu B, PereiraPerrin M A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi. Infect. Immun., 2008-06-09;76(8):3530-8. 2008-06-09 [PMID: 18541656] (PcIP, Bacteria)
• Sather S, Kenyon KD, Lefkowitz JB, Liang X, Varnum BC, Henson PM, Graham DK A soluble form of the Mer receptor tyrosine kinase inhibits macrophage clearance of apoptotic cells and platelet aggregation. Blood, 2006-10-17;109(3):1026-33. 2006-10-17 [PMID: 17047157] (Immunoprecipitation, Mouse)
• Carmillo P, Dago L, Day ES, Worley DS, Rossomando A, Walus L, Orozco O, Buckley C, Miller S, Tse A, Cate RL, Rosenblad C, Sah DW, Gronborg M, Whitty A Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin. Biochemistry, 2005-02-22;44(7):2545-54. 2005-02-22 [PMID: 15709767] (Bioassay, ELISA Developmet, Surface Plasmon Resonance, Rat)

Bioinformatics

• Gene Symbol: Ret
• Uniprot:
  • P35546.2()