Recombinant Mouse PDGF-AB Protein, CF

Recombinant Mouse PDGF-AB induces NR6R-3T3 mouse fibroblast cell proliferation. The ED50 for this effect is 15.0-75.0 ng/mL.

2 µg/lane of Recombinant Mouse PDGF-AB (Catalog # 10839-AB) was resolved by SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 17.5 kDa (A chain) & 12.5 kDa (B chain) and 29 kDa (heterodimer), respectively.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse PDGF-AB Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 15.0-75.0 ng/mL.
• Source: E. coli-derived mouse PDGF-AB protein
  

  Mouse PDGF-A (Ser87-Thr211) Accession # P20033.2

  Mouse PDGF-B (Ser82-Thr190) Accession # P31240.1
  N-terminus	C-terminus

• Accession #: P20033.2 (A chain) & P31240.1 (B chain)
• N-terminal Sequence: Ser87 (A chain) & Ser82 (B chain)
• Structure / Form: Disulfide-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 14 kDa (A chain) & 12 kDa ( beta chain).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 17.5 kDa (A chain) & 12.5 kDa (B chain), under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse PDGF-AB Protein, CF

• PDGFAB
• PDGF-AB

Background

Platelet-Derived Growth Factors (PDGFs) are a family of four cystine-knot-type growth factors (PDGF-A, -B, -C and -D) which control the growth of connective tissue cells such as fibroblasts and smooth muscle cells (1, 2). They comprise of four homodimers (PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD) and one heterodimer (PDGF-AB). All four PDGF genes contain a signal sequence, a propeptide and a mature form (2, 3). Mature mouse PDGFA is 95% and 98% identical to human and rat PDGFA respectively whereas mature mouse PDGFB is 89% and 98% homologous to human and rat PDGFB respectively. In general, PDGFs are expressed by a number of different cells and tissues including endothelial cells, epithelial cells, hematopoietic cells, connective tissue, nervous tissue, brain, muscle, kidney and liver. PDGF expression is highly regulated with PDGF levels increasing following injury and/or disease. An analysis of PDGF purified from human platelets suggest that approximately 70% of sera-derived PDGF is PDGF-AB (4). PDGF isoforms exert their cellular effect by binding to the structurally similar receptor tyrosine kinases PDGF receptor-alpha and PDGF receptor-beta. PDGF-AB binding induces receptor dimerization resulting in alpha alpha receptor homodimers and alpha beta receptor heterodimers. Binding of PDGF receptors has been reported to result in stimulation of mitogenicity and chemotaxis of fibroblasts, stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, stimulation of collagen, fibronectin, proteoglycan, and hyaluronic acid synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production (5).

1. Kohler, N. Lipton, A. (1974) Exp Cell Res. 87:297.
2. Ross, R. et al. (1974) Proc Natl Acad Sci U.S.A. 71:1207.
3. Frediksson, L. et al. (2004) Cytokine Growth Factor Rev. 15:197.
4. Chen, P-H. et al. (2013) BiochimBiophysActa 1834:2176.
5. Heldin, C-H. Eriksson, U. and Ostman, A. (2002) Arch. Biochem. Biophysic. 398:284.

Publications for PDGF-AB (10839-AB)(1)

Publication using 10839-AB

• Wang, Q;Li, H;Mao, Y;Garg, A;Park, ES;Wu, Y;Chow, A;Peregrin, J;Zhang, X; Shc1 cooperates with Frs2 and Shp2 to recruit Grb2 in FGF-induced lens development bioRxiv : the preprint server for biology 2025-01-06 [PMID: 39484547] (Bioassay, Mouse)

Bioinformatics

• Uniprot:
  • P20033.2 (A chain) & P31240.1 (B chain)()