Recombinant Mouse OMgp Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse OMgp Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200 ng on a nitrocellulose-coated microplate. All experiments were done under carrier-free conditions.
• Source: Mouse myeloma cell line, NS0-derived mouse OMgp protein Ile25-Ser420, with a C-terminal 10-His tag
• Accession #: Q63912
• N-terminal Sequence: Ile25
• Protein/Peptide Type: Recombinant Proteins
• Gene: Omg
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 45.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100-120 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse OMgp Protein, CF

• oligodendrocyte myelin glycoprotein
• OMG
• OMgp
• OMGPoligodendrocyte-myelin glycoprotein

Background

Oligodendrocyte myelin glycoprotein (OMgp or OMG), Nogo, and myelin-associated glycoprotein (MAG), are three myelin-derived axon outgrowth inhibitors that collapse axonal growth cones and inhibit neurite outgrowth (1 - 3). These three structurally distinct proteins contribute to the myelin-associated inhibitory activity that prevents axonal regeneration after injury of the adult central nervous system (CNS). Mouse OMgp cDNA encodes a 440 amino acid (aa) residue glycosylphosphatidylinositol (GPI)-anchored protein that has a 24 aa signal peptide, eight leucine-rich repeats (LRR) followed by five serine/threonine-rich repeats (4). OMgp has multiple potential N-glycosylation and O-glycosylation sites. Mouse and human OMgp share approximately 88% aa sequence identity. OMgp is expressed on the surface of oligodendrocytes and on large projection neurons, including Purkinje cells of the cerebellum, pyramidal cells of the hippocampus, motoneurons of the brainstem and anterior horn cells of the spinal cord (5). The neurite outgrowth inhibitory activities of all three myelin-derived proteins are mediated by binding to a common receptor complex consisting of the Nogo receptor (NgR) and the p75 neurotrophin receptor (NGFR) (2, 3). Besides its function in the inhibition of axonal growth, OMgp has also been implicated in the inhibition of proliferation. Although the transmembrane receptor that mediates the proliferation inhibition activity has not been identified, the LRR repeats of OMgp were shown to be essential for both the proliferation inhibition and neurite outgrowth inhibition activities (6).

1. Kottis, V. et al. (2002) J. Neurochem. 82:1566.
2. Wang, K. et al. (2002) Nature 417:941.
3. Wang, K. et al. (2002) Nature 420:74.
4. Mikol, D.D. et al. (1993) Genomics 17:604.
5. Habib, A. et al. (1998) J. Neurochem. 70:1704.
6. Vourc’h, P. et al. (2003) J. Neurochem. 85:889.

Publications for OMgp (1674-MG)(3)

Publications using 1674-MG

• Y Kawakami, Y Kurihara, Y Saito, Y Fujita, T Yamashita, K Takei The soluble form of LOTUS inhibits Nogo receptor-mediated signaling by interfering with the interaction between Nogo receptor type 1 and p75 neurotrophin receptor J. Neurosci., 2018-02-09;0(0):. 2018-02-09 [PMID: 29440387] (Bioassay, Mouse)
• Kurihara Y, Iketani M, Ito H, Nishiyama K, Sakakibara Y, Goshima Y, Takei K LOTUS suppresses axon growth inhibition by blocking interaction between Nogo receptor-1 and all four types of its ligand. Mol Cell Neurosci, 2014-07-15;61(0):211-8. 2014-07-15 [PMID: 25034269] (Bioassay, Chicken, Mouse)
• Robak LA, Venkatesh K, Lee H, Raiker SJ, Duan Y, Lee-Osbourne J, Hofer T, Mage RG, Rader C, Giger RJ Molecular basis of the interactions of the Nogo-66 receptor and its homolog NgR2 with myelin-associated glycoprotein: development of NgROMNI-Fc, a novel antagonist of CNS myelin inhibition. J. Neurosci., 2009-05-06;29(18):5768-83. 2009-05-06 [PMID: 19420245] (Bioassay, Rat)

Bioinformatics

• Gene Symbol: Omg
• Uniprot:
  • Q63912()