Recombinant Mouse NPDC-1 Fc Chimera Protein, CF

2 μg/lane of Recombinant Mouse NPDC-1 Fc Chimera Protein (Catalog # 10970-NP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-62 kDa and 108-124 kDa, respectively.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse NPDC-1 Fc Chimera Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived mouse NPDC-1 protein
  

  Mouse NPDC-1 (Ala32-Thr190, Thr33Leu) Accession # Q64322.2	IEGRMD	Human IgG1 Fc (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q64322.2
• N-terminal Sequence: Ala32 & Arg35
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 54-62 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 µg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse NPDC-1 Fc Chimera Protein, CF

• CAB-
• CAB1
• CAB-1
• CAB1CAB
• DKFZp586J0523
• neural proliferation differentiation and control protein 1
• neural proliferation, differentiation and control, 1
• NPDC1
• NPDC-1

Background

Neural proliferation and differentiation control protein 1 (NPDC1) is an O-linked glycoprotein primarily expressed in lung and neural tissues and is involved in the regulation of cellular proliferation and differentiation (1,2). Mature NPDC1 consists of an extracellular domain (ECD) with a potential HLH-like domain, a transmembrane domain and a cytoplasmic domain containing a PEST motif (rich in proline, glutamine, serine, and threonine) which targets the protein for degradation (1). The ECD of mouse NPDC1 shares 76% and 92% amino acid sequence identity with human and rat NPDC1, respectively. NPDC1 expression is developmentally regulated and persists in the adult. NPDC1 binds a variety of cyclins and regulates differentiation events in neuronal precursor cells. It is expressed when neuronal precursor cells stop to divide and begin to differentiate (3). NPDC1 can interact with the transcription factor E2F-1, which results in the inhibition of E2F-1 binding to DNA and the corresponding transcription events (4). NPDC1 has been identified as a novel ligand for PILR alpha , binding through sialylation-dependent recognition (5).

1. Spencer, M.L. et al. (2004) J. Biol. Chem. 279:37069.
2. Evrard, C. and P. Rouget (2005) J. Neurosci. Res. 79:747.
3. Galiana, E. et al. (1995) Proc. Natl. Acad. Sci. U.S.A. 92:1560.
4. Sansal, I. et al. (2000) Oncogene 19:5000.
5. Sun, Y. et al. (2012) J Biol Chem. 287:15837.

Bioinformatics

• Uniprot:
  • Q64322.2()