Recombinant Mouse Neuropoietin Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse Neuropoietin Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 50-200 ng/mL.
Source
E. coli-derived mouse Neuropoietin/NP protein
Ala23-Ala204, with and without an N-terminal Met
Accession #
N-terminal Sequence
Met & Ala23
Protein/Peptide Type
Recombinant Proteins
Gene
Ctf2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
2709-NP/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Neuropoietin Protein, CF

  • Cardiotrophin-2
  • CTF2P
  • Neuropoietin
  • NP

Background

Neuropoietin (NP; also known as cardiotrophin-2) is a 22 kDa member of the IL-6 family of cytokines. Considered to be the product of a gene duplication event involving cardiotrophin-1 (CT-1), it helps to define a subfamily within the IL-6 family that includes CT-1, CLC and CTNF. Mouse neuropoietin is synthesized as a 204 amino acid (aa) precursor that contains a 22 aa signal sequence and a 192 aa mature segment. The secreted molecule is characterized by the presence of four alpha -helices, typical of hematopoietic superfamily molecules. Mature mouse neuropoietin shares 88%, 90% and 95% aa identity to chimpanzee, canine and rat neuropoietin, respectively. The human gene is suggested to have evolved towards a pseudogene, a point of interest in that neuropoietin is reported to signal through the CNTF complex (i.e., gp130, CNTF R alpha and LIF R). NP will mediate motor neuron survival, and appears to be selectively expressed in the embryo by tissues involved with nervous system development (1).

  1. Derouet, D. et al., 2004, Proc. Natl. Acad. Sci. USA 101:4827.

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Publications for Neuropoietin/NP (2709-NP/CF)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioassay
(1)
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Human
(1)
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Bioinformatics

Gene Symbol Ctf2
Uniprot