Recombinant Mouse Neuropoietin Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Neuropoietin Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 50-200 ng/mL.
• Source: E. coli-derived mouse Neuropoietin/NP protein
  Ala23-Ala204, with and without an N-terminal Met
• Accession #: P83714
• N-terminal Sequence: Met & Ala23
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ctf2
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Neuropoietin Protein, CF

• Cardiotrophin-2
• CTF2P
• Neuropoietin
• NP

Background

Neuropoietin (NP; also known as cardiotrophin-2) is a 22 kDa member of the IL-6 family of cytokines. Considered to be the product of a gene duplication event involving cardiotrophin-1 (CT-1), it helps to define a subfamily within the IL-6 family that includes CT-1, CLC and CTNF. Mouse neuropoietin is synthesized as a 204 amino acid (aa) precursor that contains a 22 aa signal sequence and a 192 aa mature segment. The secreted molecule is characterized by the presence of four alpha -helices, typical of hematopoietic superfamily molecules. Mature mouse neuropoietin shares 88%, 90% and 95% aa identity to chimpanzee, canine and rat neuropoietin, respectively. The human gene is suggested to have evolved towards a pseudogene, a point of interest in that neuropoietin is reported to signal through the CNTF complex (i.e., gp130, CNTF R alpha and LIF R). NP will mediate motor neuron survival, and appears to be selectively expressed in the embryo by tissues involved with nervous system development (1).

1. Derouet, D. et al., 2004, Proc. Natl. Acad. Sci. USA 101:4827.

Publications for Neuropoietin/NP (2709-NP/CF)(1)

Publication using 2709-NP/CF

• Larsen JV, Hansen M, Moller B, Madsen P, Scheller J, Nielsen M, Petersen CM Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer. Mol. Cell. Biol., 2010;17(0):4175. 2010 [PMID: 20584990] (Bioassay, Human)

Bioinformatics

• Gene Symbol: Ctf2
• Entrez:
  • 647088(Human)
  • 244218(Mouse)
• Uniprot:
  • P83714()