Reactivity | MuSpecies Glossary |
Applications | Bioactivity |
Format | Carrier-Free |
Details of Functionality | Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 50-200 ng/mL. |
Source | E. coli-derived mouse Neuropoietin/NP protein Ala23-Ala204, with and without an N-terminal Met |
Accession # | |
N-terminal Sequence | Met & Ala23 |
Protein/Peptide Type | Recombinant Proteins |
Gene | Ctf2 |
Purity | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Endotoxin Note | <1.0 EU per 1 μg of the protein by the LAL method. |
Dilutions |
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Theoretical MW | 20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Reconstitution Instructions | Reconstitute at 100 μg/mL in sterile PBS. |
Neuropoietin (NP; also known as cardiotrophin-2) is a 22 kDa member of the IL-6 family of cytokines. Considered to be the product of a gene duplication event involving cardiotrophin-1 (CT-1), it helps to define a subfamily within the IL-6 family that includes CT-1, CLC and CTNF. Mouse neuropoietin is synthesized as a 204 amino acid (aa) precursor that contains a 22 aa signal sequence and a 192 aa mature segment. The secreted molecule is characterized by the presence of four alpha -helices, typical of hematopoietic superfamily molecules. Mature mouse neuropoietin shares 88%, 90% and 95% aa identity to chimpanzee, canine and rat neuropoietin, respectively. The human gene is suggested to have evolved towards a pseudogene, a point of interest in that neuropoietin is reported to signal through the CNTF complex (i.e., gp130, CNTF R alpha and LIF R). NP will mediate motor neuron survival, and appears to be selectively expressed in the embryo by tissues involved with nervous system development (1).
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