Recombinant Mouse KLRG1 Fc Chimera Protein, CF

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WhenRecombinant Mouse E‑Cadherin (Catalog # 8875‑EC) is coated at 5 µg/mL, 100 μL/well, Recombinant Mouse KLRG1 FcChimera (Catalog # 6944‑KR) binds withan ED50 of 15‑90 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse KLRG1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Mouse E-Cadherin (Catalog # 8875-EC) is immobilized at 5 μg/mL (100 μL/well), the concentration of Recombinant Mouse KLRG1 Fc Chimera that produces 50% of the optimal binding response is 15-90 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse KLRG1 protein
MDP Mouse IgG2A
(Glu98-Lys330)
IEGR Mouse KLRG1
(Gln57-Tyr188)
Accession # O88713
N-terminus C-terminus
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Klrg1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
42.2 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
45-57 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse KLRG1 Fc Chimera Protein, CF

  • 2F1
  • CLEC15A
  • CLEC15AMAFA-LIKE
  • C-type lectin domain family 15 member A
  • C-type lectin domain family 15, member A
  • ITIM-containing receptor MAFA-L
  • killer cell lectin-like receptor subfamily G member 1
  • killer cell lectin-like receptor subfamily G, member 1
  • KLRG1
  • MAFA
  • MAFAL
  • MAFA-L
  • MAFA-like receptor
  • MAFAMAFA-2F1
  • mast cell function-associated antigen (ITIM-containing)
  • Mast cell function-associated antigen
  • MGC13600

Background

KLRG1 (killer cell lectin‑like receptor G1), also called MAFA (mast cell function associated), is an inhibitory type II transmembrane glycoprotein of the C‑type lectin family, designated CLEC15A (1). Mature mouse KLRG1 consists of a 33 amino acid (aa) cytoplasmic domain with one immunoreceptor tyrosine‑based inhibitory motif (ITIM), a 23 aa transmembrane segment, and a 132 aa extracellular domain (ECD) with one C‑type lectin domain (CTLD) (2). Within the ECD, mouse KLRG1 shares 57% and 80% aa sequence identity with human and rat KLRG1, respectively. Alternate splicing generates additional isoforms of mouse KLRG1 that lack either the CTLD or the CTLD, transmembrane segment, and a portion of the cytoplasmic domain (3). KLRG1 is expressed as a 30 ‑ 40 kDa N‑glycosylated molecule that forms disulfide‑linked homodimers, trimers, and tetramers (4, 5). It is expressed on subpopulations of CD8+, CD4+, regulatory, and gamma/delta T cells as well as on NK cells (2, 4, 6 ‑ 8). KLRG1 is expressed on T cells found in cord blood, but it is down‑regulated postnatally and is subsequently re‑expressed on antigen‑exposed T cells (7, 9). It is expressed by a greater proportion of CD8+ T cells in the elderly and by virus‑specific CD8+ T cells during chronic virus infection (10 ‑ 12). KLRG1 binds to E-, N-, and R-Cadherins, triggering ITIM‑dependent KLRG1 signaling and inhibition of T cell activation (5, 13, 14). The response is bi‑directional, as KLRG1 binding to E‑Cadherin on dendritic cells (DC) can induce an anti‑inflammatory DC phenotype (increased IL‑10 production and decreased IL‑6 and TNF‑ alpha production) (15).

  1. Henson, S.M. and A.N. Akbar (2009) Age 31:285.
  2. Hanke, T. et al. (1998) Eur. J. Immunol. 28:4409.
  3. Voehringer, D. et al. (2001) Immunogenetics 52:206.
  4. Corral, L. et al. (2000) Eur. J. Immunol. 30:920.
  5. Rosshart, S. et al. (2008) Eur. J. Immunol. 38:3354.
  6. Voehringer, D. et al. (2002) Blood 100:3698.
  7. Beyersdorf, N. et al. (2007) Eur. J. Immunol. 37:3445.
  8. Eberl, M. et al. (2005) J. Leukoc. Biol. 77:67.
  9. Marcolino, I. et al. (2004) Eur. J. Immunol. 34:2672.
  10. Ouyang, Q. et al. (2003) Exp. Gerontol. 38:911.
  11. Thimme, R. et al. (2005) J. Virol. 79:12112.
  12. Cush, S.S. and E. Flano (2011) J. Immunol. 186:4051.
  13. Ito, M. et al. (2006) J. Exp. Med. 203:289.
  14. Tessmer, M.S. et al. (2007) Int. Immunol. 19:391.
  15. Banh, C. et al. (2009) Blood 114:5299.

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Bioinformatics

Gene Symbol Klrg1
Uniprot
COVID-19 update