Recombinant Mouse Integrin alpha 6 beta 4 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Integrin alpha 6 beta 4 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Mouse Laminin I (Catalog # 3400-010-01) is coated at 10 μg/mL, Recombinant Mouse Integrin alpha 6 beta 4 binds with an apparent K_D <10 nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 6 beta 4 protein
  

  Mouse Integrin alpha 6 (Phe24-Ser1011) Accession # Q61739	His-Pro	GGGSGGGS	Acidic Tail	6-His tag
  Mouse Integrin beta 4 (Asn29-Ser711) Accession # NP_598424	His-Pro	GGGSGGGS	Basic Tail
  N-terminus				C-terminus

• Accession #: Q61739 (Integrin alpha 6) & NP_598424 (Integrin beta 4)
• N-terminal Sequence: Phe24 (Integrin alpha 6) & Asn29 (Integrin beta 4)
• Structure / Form: Noncovalently-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Itgae
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 119 kDa (Integrin alpha 6) & 84.6 kDa (Integrin beta 4).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100-115 kDa & 135-145 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Integrin alpha 6 beta 4 Protein, CF

• Integrin alpha 6 beta 4

Background

Integrin alpha 6 beta 4 is primarily an epithelial and Schwann cell laminin-binding integrin. While the alpha 6 subunit can also pair with beta 1, beta 4 pairs only with alpha 6 (1, 2). Expression of the non-covalent heterodimer composed of ~150 kDa alpha 6/CD49f and 150-200 kDa beta 4/CD104 type I transmembrane glycoprotein subunits is required for hemidesmosome formation (1, 3). The alpha 6 subunit contains an I (inhibitory) domain and a cleavage site that creates extracellular domain (ECD) heavy and transmembrane light chains. Mouse and human ubiquitously express the X1 isoform, while alternate splicing in the human ECD also creates X2 and X1X2 isoforms. Cytoplasmic splicing creates A and B isoforms in both mouse and human (4, 5). The beta 4 subunit cytoplasmic domain is unusually long (~1000 aa) and contains four type III fibronectin repeats that bind intracellular hemidesmosomal components (1-4). beta 4 alternative splicing between repeats 2 and 3 creates isoform 2 (deletion of 65 aa) and 3 (deletion plus insertion of 52 aa), which differ in tissue distribution (2, 5). The 876 aa mouse alpha 6 heavy chain shares 98% aa sequence identity with rat and 92-93% with human (X1), bovine, and canine  alpha 6. The 684 aa mouse beta 4 ECD shares 96% aa sequence identity with rat and 87‑92% with human, bovine, and equine  beta 4. Mutation of alpha 6 beta 4 can cause EB-PA, or epidermolysis bullosa (detachment of epidermis from basement membrane) with pyloric atresia, that is neonatally lethal in humans if severe (1, 3, 5). On Schwann cells, alpha 6 beta 4 cooperates with dystroglycan to stabilize the myelin sheath, and mediates attachment to the basal lamina (6, 7). alpha 6 beta 4 is also expressed on vessel‑associated muscle progenitors and on lung vascular endothelial cells, where it binds HLA class I molecules and enhances antigen presentation and cell proliferation (8‑10). High alpha 6 beta 4 expression correlates with invasiveness of carcinomas (1). In carcinomas, it binds IGF-I and the tetraspanin CD151, which promotes phosphorylation of beta 4 by EGF R, disrupting hemidesmosomes and allowing tumor cell migration (1, 11‑14). alpha 6 beta 4 signaling can also amplify tumor production of VEGF, ErbB and SPARC proteins (1, 15‑17).

1. Wilhelmsen, K. et al. (2006) Mol. Cell. Biol. 26:2877.
2. Kennel, S.J. et al. (1993) Gene 130:209.
3. Schaapveld, R. Q. J. et al. (1998) J. Cell Biol. 142:271.
4. van Leusden, M. R. et al. (1997) Biochem. Biophys. Res. Commun. 235:826.
5. Pulkkinen, L. et al. (1998) Am. J. Hum. Genet. 63:1376.
6. Nodari, A. et al. (2008) J. Neurosci. 28:6714.
7. Amici, S. A. et al. (2006) J. Neurosci. 26:1179.
8. Liadaki, K. et al. (2012) J. Histochem. Cytochem. 60:31.
9. Zhang, X. and E.F. Reed (2012) Hum. Immunol. 73:1239.
10. Liu, C. et al. (2012) PLoS ONE 7:e32060.
11. Fujita, M. et al. (2012) J. Biol. Chem. 287:12491.
12. Wilhelmsen, K. et al. (2007) Mol. Biol. Cell 18:3512.
13. Yang, X. H. et al. (2008) Cancer Res. 68:3204.
14. Frijns, E. et al. (2010) J. Biol. Chem. 285:37650.
15. Lipscomb, E. A. et al. (2005) Cancer Res. 65:10970.
16. Folgiero, V. et al. (2007) Cancer Res. 67:1645.
17. Gerson, K.D. et al. (2012) J. Biol. Chem. 287:9835.

Publications for Integrin alpha 6 beta 4 (8067-A6)(1)

Publication using 8067-A6

• Takeda, K;Kaifu, T;Michihata, R;Kinugawa, N;Fujioka, A;Tateno, A;Toshima, K;Kanoh, H;Inamori, KI;Kamijo, K;Himeda, T;Ohara, Y;Inokuchi, JI;Nakamura, A; Chronic encephalomyelitis virus exhibits cellular tropism and evades pDCs by binding to sialylated integrins as the cell surface receptors European journal of immunology 2023-08-11 [PMID: 37565654] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Itgae
• Uniprot:
  • Q61739 (Integrin alpha 6) & NP_598424 (Integrin beta 4)()