Recombinant Mouse IL-3R beta Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IL-3R beta Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the IL-3-induced proliferation of NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 0.2‑1 µg/mL in the presence of 2.5 µg/mL soluble rmIL-3 R alpha /Fc Chimera and 0.5 ng/mL of rmIL-3..
• Source: Mouse myeloma cell line, NS0-derived mouse IL-3 R beta protein
  

  Mouse IL-3 R beta (His23 - Trp440) Accession # Q3U3P4	IEGRID	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: Q3U3P4
• N-terminal Sequence: His23
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Csf2rb2
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 74.9 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 80 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-3R beta Fc Chimera Protein, CF

• AIC2A
• BetaIl3
• Bil3
• Csfgmrb
• IL3R beta
• IL-3R beta
• IL3R
• IL3RB
• IL-3Rb
• Il3rb2

Background

Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta . AIC2A was found to be the result of a gene duplication event. Both the alpha and the beta  subunits are members of the cytokine receptor superfamily (3).

1. Itoh, N. et al. (1990) Science 247:324.
2. Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
3. Schrader, J.W. in Cytokine Reference, (2001) J.J. Oppenheim and M. Feldmann, eds. Academic Press, New York, p. 1899.

Bioinformatics

• Gene Symbol: Csf2rb2
• Uniprot:
  • Q3U3P4()