Recombinant Mouse IL-11 Protein

Product Discontinued

Recombinant Mouse IL-11 Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using T11 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED₅₀ for this effect is 0.04-0.4 ng/mL.
• Source: E. coli-derived mouse IL-11 protein
  Gly23-Leu199, with an N-terminal Met and Pro25-Leu199
• Accession #: P47873
• N-terminal Sequence: Met & Pro25
• Protein/Peptide Type: Recombinant Proteins
• Gene: Il11
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80 with Trehalose and with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-11 Protein

• Adipogenesis inhibitory factor
• AGIF
• AGIFoprelvekin
• IL11
• IL-11
• IL-11Oprelvekin
• interleukin 11
• interleukin-11
• Oprelvekin

Background

IL-11 (Interleukin 11) is a pleiotropic cytokine in the IL-6 family, which also includes LIF, CNTF, Oncostatin M, Cardiotrophin-1, IL-27 and IL-31 (1-4). In humans, IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF) (3). The mouse IL-11 cDNA encodes a 199 amino acid (aa) precursor, which generates a 178 aa, 19 kDa mature unglycosylated protein. Mature mouse IL-11 shares 88%, 97%, and 89% aa sequence identity with human, rat and canine IL-11, respectively. IL-11 is secreted by osteoblasts, synoviocytes, fibroblasts, chondrocytes, intestinal myofibroblasts, and trophoblasts, among other cell types (1). It is found in the plasma mainly during inflammation, such as that associated with viral infection, cancer, or inflammatory arthritis, and is considered to be primarily anti‑inflammatory (1). It stimulates hematopoiesis and thrombopoiesis, regulates macrophage differentiation, and confers mucosal protection in the intestine (1). It has also been found to enhance T cell polarization toward Th2, promote B cell IgG production, increase osteoclast bone absorption, protect endothelial cells from oxidative stress, and regulate epithelial proliferation and apoptosis (1). IL-11 synergizes with several other cytokines to produce these effects, and its effects overlap with those of IL-6 (1). IL-11 receptor activation requires formation of a complex of two IL-11 molecules with two molecules of the ligand-binding IL-11 R alpha subunit and two molecules of the ubiquitously expressed cell signaling beta subunit, gp130 (5). A soluble form of IL-11 R alpha can bind IL-11 and either form a signaling complex with gp130 on the cell surface, or inhibit cell surface IL-11 R alpha /gp130 signaling (6-8).

1. Putoczki, T. and M. Ernst (2010) J. Leukoc. Biol. 88:1109.
2. Paul, S.R. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7512.
3. Kawashima, I. et al. (1991) FEBS Lett. 283:199.
4. Morris, J.C. et al. (1996) Exp. Hematol. 24:1369.
5. Barton, V.A. et al. (2000) J. Biol. Chem. 275:36197.
6. Curtis, D.J. et al. (1997) Blood 90:4403.
7. Baumann, H. et al. (1996) J. Immunol. 157:284.
8. Karow, J. et al. (1996) Biochem. J. 318:489.

Publications for IL-11 (418-ML)(28)

Publications using 418-ML

• Bréart, B;Williams, K;Krimm, S;Wong, T;Kayser, BD;Wang, L;Cheng, E;Cruz Tleugabulova, M;Bouziat, R;Lu, T;Yuen, K;Firmino, NS;Bravo, DD;Roels, J;Bhakta, A;Bevers, J;Lehoux, I;Gutierrez, A;Chestnut, Y;Klementowicz, JE;Arenzana, TL;Akhmetzyanova, I;Dixon, E;Chen, M;Tasneem, K;Yadav, R;Koeppen, H;Oh, SA;Delamarre, L;Huang, H;Lim, SA;Nakamura, G;Wang, J;Gao, C;Corpuz, R;Müller, S;West, NR; IL-27 elicits a cytotoxic CD8+ T cell program to enforce tumour control Nature 2025-02-05 [PMID: 39910298] (Bioassay, Mouse, Transgenic Mouse)
• J Wu, K Krchma, HJ Lee, S Prabhakar, X Wang, H Zhao, X Xing, RH Seong, DH Fremont, MN Artyomov, T Wang, K Choi Requisite Chromatin Remodeling for Myeloid and Erythroid Lineage Differentiation from Erythromyeloid Progenitors Cell Rep, 2020-11-17;33(7):108395. 2020-11-17 [PMID: 33207205] (Cell Culture, Mouse)
• Wenlong Yang, Shuning Zhang, Tiantong Ou, Hao Jiang, Daile Jia, Zhiyong Qi et al. Interleukin-11 regulates the fate of adipose-derived mesenchymal stem cells via STAT3 signalling pathways Cell Proliferation 2020-05-01 [PMID: 32270546] (Bioassay, Mouse)
• X Zhang, N Kiapour, S Kapoor, T Khan, M Thamilaras, Y Tao, S Cohen, R Miller, RA Sobel, S Markovic-P IL-11 Induces Encephalitogenic Th17 Cells in Multiple Sclerosis and Experimental Autoimmune Encephalomyelitis J. Immunol., 2019-07-24;0(0):. 2019-07-24 [PMID: 31341075] (Bioassay, Mouse)
• MJ Delás, BT Jackson, T Kovacevic, S Vangelisti, E Munera Mar, SA Wild, EM Stork, N Erard, SRV Knott, GJ Hannon lncRNA Spehd Regulates Hematopoietic Stem and Progenitor Cells and Is Required for Multilineage Differentiation Cell Rep, 2019-04-16;27(3):719-729.e6. 2019-04-16 [PMID: 30995471] (Cell Culture, Mouse)
• KR Wilson, IH Kang, U Baliga, Y Xiong, S Chatterjee, E Moore, B Parthiban, K Thyagaraja, JL Borke, S Mehrotra, KL Kirkwood, AC LaRue, M Ogawa, M Mehrotra Hematopoietic Stem Cells as a Novel Source of Dental Tissue Cells Sci Rep, 2018-05-23;8(1):8026. 2018-05-23 [PMID: 29795229] (Bioassay, Mouse)
• JS Dahlin, FK Hamey, B Pijuan-Sal, M Shepherd, WWY Lau, S Nestorowa, C Weinreb, S Wolock, R Hannah, E Diamanti, DG Kent, B Göttgens, NK Wilson A single cell hematopoietic landscape resolves eight lineage trajectories and defects in Kit mutant mice Blood, 2018-03-27;0(0):. 2018-03-27 [PMID: 29588278] (Bioassay, Mouse)
• JE Draper, P Sroczynska, MZH Fadlullah, R Patel, G Newton, W Breitwiese, V Kouskoff, G Lacaud A novel prospective isolation of murine fetal liver progenitors to study in utero hematopoietic defects PLoS Genet., 2018-01-04;14(1):e1007127. 2018-01-04 [PMID: 29300724] (Bioassay, Mouse)
• S Ollila, E Domènech-M, K Laajanen, IP Wong, S Tripathi, N Pentinmikk, Y Gao, Y Yan, EH Niemelä, TC Wang, B Viollet, G Leone, P Katajisto, K Vaahtomeri, TP Mäkelä Stromal Lkb1 deficiency leads to gastrointestinal tumorigenesis involving the IL-11-JAK/STAT3 pathway J. Clin. Invest., 2017-12-04;0(0):. 2017-12-04 [PMID: 29202476] (Bioassay, Mouse)
• S Shukla, MA Langley, J Singh, JM Edgar, M Mohtashami, JC Zúñiga-Pfl, PW Zandstra Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1 Nat. Methods, 2017-04-10;14(5):531-538. 2017-04-10 [PMID: 28394335] (Bioassay, Mouse)
• Franziska Ruf Loss of Sfrp2 in the Niche Amplifies Stress-Induced Cellular Responses, and Impairs the in Vivo Regeneration of the Hematopoietic Stem Cell Pool Stem Cells, 2016-06-30;0(0):. 2016-06-30 [PMID: 27299503] (Bioassay, Mouse)
• Gene Therapy Induces Antigen-Specific Tolerance in Experimental Collagen-Induced Arthritis PLoS ONE, 2016-05-09;11(5):e0154630. 2016-05-09 [PMID: 27159398] (Bioassay, Mouse)
• Seto D, Kandarian S, Jackman R A Key Role for Leukemia Inhibitory Factor in C26 Cancer Cachexia. J Biol Chem, 2015-06-19;290(32):19976-86. 2015-06-19 [PMID: 26092726] (Bioassay, Mouse)
• Miyawaki K, Arinobu Y, Iwasaki H, Kohno K, Tsuzuki H, Iino T, Shima T, Kikushige Y, Takenaka K, Miyamoto T, Akashi K CD41 marks the initial myelo-erythroid lineage specification in adult mouse hematopoiesis: redefinition of murine common myeloid progenitor. Stem Cells, 2015-03-01;33(3):976-87. 2015-03-01 [PMID: 25446279] (Bioassay, Mouse)
• Figueiredo C, Drohomyrecky P, McCarthy S, Leontyev D, Ma X, Branch D, Dunn S Optimal attenuation of experimental autoimmune encephalomyelitis by intravenous immunoglobulin requires an intact interleukin-11 receptor. PLoS ONE, 2014-07-31;9(7):e101947. 2014-07-31 [PMID: 25078447] (Bioassay, Mouse)
• Carnevalli L, Scognamiglio R, Cabezas-Wallscheid N, Rahmig S, Laurenti E, Masuda K, Jockel L, Kuck A, Sujer S, Polykratis A, Erlacher M, Pasparakis M, Essers M, Trumpp A Improved HSC reconstitution and protection from inflammatory stress and chemotherapy in mice lacking granzyme B. J Exp Med, 2014-04-21;211(5):769-79. 2014-04-21 [PMID: 24752302] (Bioassay, Mouse)
• Verovskaya , Evgenia, Broekhuis , Mathilde, Zwart , Erik, Weersing , Ellen, Ritsema , Martha, Bosman , Lisette, van Poele , Theo, de Haan , Gerald, Bystrykh , Leonid V Asymmetry in skeletal distribution of mouse hematopoietic stem cell clones and their equilibration by mobilizing cytokines. J Exp Med, 2014-02-24;211(3):487-97. 2014-02-24 [PMID: 24567446] (Bioassay, Mouse)
• Elsaeidi , Fairouz, Bemben , Michael, Zhao , Xiao-Fen, Goldman , Daniel Jak/Stat signaling stimulates zebrafish optic nerve regeneration and overcomes the inhibitory actions of Socs3 and Sfpq. J Neurosci, 2014-02-12;34(7):2632-44. 2014-02-12 [PMID: 24523552] (Bioassay, Zebrafish)
• Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M Identification of transcription factors for lineage-specific ESC differentiation. Stem Cell Reports, 2013-11-27;1(6):545-59. 2013-11-27 [PMID: 24371809] (Bioassay, Mouse)
• Regulation of innate CD8+ T-cell activation mediated by cytokines. Proc Natl Acad Sci U S A, 2012-06-04;109(25):9971-6. 2012-06-04 [PMID: 22665806] (Bioassay, Mouse)
• Cook BD, Liu S, Evans T Smad1 signaling restricts hematopoietic potential after promoting hemangioblast commitment. Blood, 2011-04-22;117(24):6489-97. 2011-04-22 [PMID: 21515822] (Bioassay, Mouse)
• Mitchell HM, White DM, Domowicz MS, Kraig RP Cold pre-conditioning neuroprotection depends on TNF-alpha and is enhanced by blockade of interleukin-11. J. Neurochem., 2010-12-01;117(2):187-96. 2010-12-01 [PMID: 21070241] (Bioassay, Rat)
• Stumhofer JS, Tait ED, Quinn WJ, Hosken N, Spudy B, Goenka R, Fielding CA, O'Hara AC, Chen Y, Jones ML, Saris CJ, Rose-John S, Cua DJ, Jones SA, Elloso MM, Grotzinger J, Cancro MP, Levin SD, Hunter CA A role for IL-27p28 as an antagonist of gp130-mediated signaling. Nat. Immunol., 2010-11-07;11(12):1119-26. 2010-11-07 [PMID: 21057510] (Bioassay, Mouse)
• Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus. J. Virol., 2010-09-29;84(24):12703-12. 2010-09-29 [PMID: 20881038] (In Vivo, Mouse)
• Krejci P, Prochazkova J, Bryja V, Jelinkova P, Pejchalova K, Kozubik A, Thompson LM, Wilcox WR Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes. Cell. Signal., 2008-10-12;21(1):151-60. 2008-10-12 [PMID: 18950705] (Bioassay, Rat)
• Zhang Y, Taveggia C, Melendez-Vasquez C, Einheber S, Raine CS, Salzer JL, Brosnan CF, John GR Interleukin-11 potentiates oligodendrocyte survival and maturation, and myelin formation. J. Neurosci., 2006-11-22;26(47):12174-85. 2006-11-22 [PMID: 17122042] (Bioassay, Mouse)
• Braun BS, Archard JA, Van Ziffle JA, Tuveson DA, Jacks TE, Shannon K Somatic activation of a conditional KrasG12D allele causes ineffective erythropoiesis in vivo. Blood, 2006-05-23;108(6):2041-4. 2006-05-23 [PMID: 16720837] (Bioassay, Mouse)
• Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice. Arch. Dermatol. Res., 2001-11-01;293(10):508-14. 2001-11-01 [PMID: 11820727] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Il11
• Uniprot:
  • P47873()