Recombinant Mouse IL-11 Protein

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse IL-11 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using T11 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED50 for this effect is 0.04-0.4 ng/mL.
Source
E. coli-derived mouse IL-11 protein
Gly23-Leu199, with an N-terminal Met and Pro25-Leu199
Accession #
N-terminal Sequence
Met & Pro25
Protein/Peptide Type
Recombinant Proteins
Gene
Il11
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
418-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80 with Trehalose and with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-11 Protein

  • Adipogenesis inhibitory factor
  • AGIF
  • AGIFoprelvekin
  • IL11
  • IL-11
  • IL-11Oprelvekin
  • interleukin 11
  • interleukin-11
  • Oprelvekin

Background

IL-11 (Interleukin 11) is a pleiotropic cytokine in the IL-6 family, which also includes LIF, CNTF, Oncostatin M, Cardiotrophin-1, IL-27 and IL-31 (1-4). In humans, IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF) (3). The mouse IL-11 cDNA encodes a 199 amino acid (aa) precursor, which generates a 178 aa, 19 kDa mature unglycosylated protein. Mature mouse IL-11 shares 88%, 97%, and 89% aa sequence identity with human, rat and canine IL-11, respectively. IL-11 is secreted by osteoblasts, synoviocytes, fibroblasts, chondrocytes, intestinal myofibroblasts, and trophoblasts, among other cell types (1). It is found in the plasma mainly during inflammation, such as that associated with viral infection, cancer, or inflammatory arthritis, and is considered to be primarily anti‑inflammatory (1). It stimulates hematopoiesis and thrombopoiesis, regulates macrophage differentiation, and confers mucosal protection in the intestine (1). It has also been found to enhance T cell polarization toward Th2, promote B cell IgG production, increase osteoclast bone absorption, protect endothelial cells from oxidative stress, and regulate epithelial proliferation and apoptosis (1). IL-11 synergizes with several other cytokines to produce these effects, and its effects overlap with those of IL-6 (1). IL-11 receptor activation requires formation of a complex of two IL-11 molecules with two molecules of the ligand-binding IL-11 R alpha subunit and two molecules of the ubiquitously expressed cell signaling beta subunit, gp130 (5). A soluble form of IL-11 R alpha can bind IL-11 and either form a signaling complex with gp130 on the cell surface, or inhibit cell surface IL-11 R alpha /gp130 signaling (6-8).

  1. Putoczki, T. and M. Ernst (2010) J. Leukoc. Biol. 88:1109.
  2. Paul, S.R. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7512.
  3. Kawashima, I. et al. (1991) FEBS Lett. 283:199.
  4. Morris, J.C. et al. (1996) Exp. Hematol. 24:1369.
  5. Barton, V.A. et al. (2000) J. Biol. Chem. 275:36197.
  6. Curtis, D.J. et al. (1997) Blood 90:4403.
  7. Baumann, H. et al. (1996) J. Immunol. 157:284.
  8. Karow, J. et al. (1996) Biochem. J. 318:489.

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Publications for IL-11 (418-ML)(25)

We have publications tested in 3 confirmed species: Mouse, Rat, Zebrafish.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.


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Bioassay
(23)
Cell Culture
(1)
In Vivo
(1)
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Mouse
(22)
Rat
(2)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using 418-ML Applications Species
X Zhang, N Kiapour, S Kapoor, T Khan, M Thamilaras, Y Tao, S Cohen, R Miller, RA Sobel, S Markovic-P IL-11 Induces Encephalitogenic Th17 Cells in Multiple Sclerosis and Experimental Autoimmune Encephalomyelitis J. Immunol., 2019;0(0):. 2019 [PMID: 31341075] (Bioassay, Mouse) Bioassay Mouse
MJ Delás, BT Jackson, T Kovacevic, S Vangelisti, E Munera Mar, SA Wild, EM Stork, N Erard, SRV Knott, GJ Hannon lncRNA Spehd Regulates Hematopoietic Stem and Progenitor Cells and Is Required for Multilineage Differentiation Cell Rep, 2019;27(3):719-729.e6. 2019 [PMID: 30995471] (Cell Culture, Mouse) Cell Culture Mouse
KR Wilson, IH Kang, U Baliga, Y Xiong, S Chatterjee, E Moore, B Parthiban, K Thyagaraja, JL Borke, S Mehrotra, KL Kirkwood, AC LaRue, M Ogawa, M Mehrotra Hematopoietic Stem Cells as a Novel Source of Dental Tissue Cells Sci Rep, 2018;8(1):8026. 2018 [PMID: 29795229] (Bioassay, Mouse) Bioassay Mouse
JS Dahlin, FK Hamey, B Pijuan-Sal, M Shepherd, WWY Lau, S Nestorowa, C Weinreb, S Wolock, R Hannah, E Diamanti, DG Kent, B Göttgens, NK Wilson A single cell hematopoietic landscape resolves eight lineage trajectories and defects in Kit mutant mice Blood, 2018;0(0):. 2018 [PMID: 29588278] (Bioassay, Mouse) Bioassay Mouse
JE Draper, P Sroczynska, MZH Fadlullah, R Patel, G Newton, W Breitwiese, V Kouskoff, G Lacaud A novel prospective isolation of murine fetal liver progenitors to study in utero hematopoietic defects PLoS Genet., 2018;14(1):e1007127. 2018 [PMID: 29300724] (Bioassay, Mouse) Bioassay Mouse
S Ollila, E Domènech-M, K Laajanen, IP Wong, S Tripathi, N Pentinmikk, Y Gao, Y Yan, EH Niemelä, TC Wang, B Viollet, G Leone, P Katajisto, K Vaahtomeri, TP Mäkelä Stromal Lkb1 deficiency leads to gastrointestinal tumorigenesis involving the IL-11-JAK/STAT3 pathway J. Clin. Invest., 2017;0(0):. 2017 [PMID: 29202476] (Bioassay, Mouse) Bioassay Mouse
S Shukla, MA Langley, J Singh, JM Edgar, M Mohtashami, JC Zúñiga-Pfl, PW Zandstra Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1 Nat. Methods, 2017;14(5):531-538. 2017 [PMID: 28394335] (Bioassay, Mouse) Bioassay Mouse
Franziska Ruf Loss of Sfrp2 in the Niche Amplifies Stress-Induced Cellular Responses, and Impairs the in Vivo Regeneration of the Hematopoietic Stem Cell Pool Stem Cells, 2016;0(0):. 2016 [PMID: 27299503] (Bioassay, Mouse) Bioassay Mouse
Gene Therapy Induces Antigen-Specific Tolerance in Experimental Collagen-Induced Arthritis PLoS ONE, 2016;11(5):e0154630. 2016 [PMID: 27159398] (Bioassay, Mouse) Bioassay Mouse
Seto D, Kandarian S, Jackman R A Key Role for Leukemia Inhibitory Factor in C26 Cancer Cachexia. J Biol Chem, 2015;290(32):19976-86. 2015 [PMID: 26092726] (Bioassay, Mouse) Bioassay Mouse
Show All 25 Publications.

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Bioinformatics

Gene Symbol Il11
Uniprot