Recombinant Mouse IFN-gamma R2 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse IFN-gamma R2 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Mouse IFN-gamma R1 Fc Chimera (Catalog # 1026-GR) is immobilized at 2 μg/mL (100 μL/well), it binds to Recombinant Mouse IFN‑ gamma R2 in the presence of Recombinant Mouse IFN-gamma (Catalog # 485-MI). The concentration of Recombinant Mouse IFN‑ gamma R2 that produces 50% of the optimal binding response was found to be approximately 0.8-4 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IFN-gamma R2 protein
Met1-Val243, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ser21
Protein/Peptide Type
Recombinant Proteins
Gene
Ifngr2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
26 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
37-50 kDa, reducing conditions and visualized by silver stain.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IFN-gamma R2 Protein, CF

  • AF-1
  • AF-1interferon-gamma receptor beta chain precursor10IFNGT1Interferon gamma receptor accessory factor 1
  • IFGR2
  • IFN-gamma R2
  • IFN-gamma receptor 2
  • IFNgammaR2
  • IFN-gamma-R2
  • IFNGR2
  • IFN-gR2
  • IFNGT1
  • interferon gamma receptor 2 (interferon gamma transducer 1)
  • interferon gamma receptor 2
  • interferon gamma receptor accessory factor-1
  • interferon gamma receptor beta chain
  • Interferon gamma transducer 1

Background

IFN‑ gamma  R2 (Interferon gamma receptor 2; also called IFN‑ gamma R beta IFN‑ gamma  RII, or AF1) is a 60‑64 kDa type I transmembrane glycoprotein that is a member of the class II cytokine receptor family of molecules (1). It is widely expressed as part of a preassembled cell surface multimeric complex. In the absence of IFN‑ gamma , the complex contains two each of IFN‑ gamma  R1, R2 and Jak1 molecules (2). Binding of IFN‑ gamma to IFN‑ gamma  R1 recruits Jak2 to IFN‑ gamma  R2 and initiates phosphorylation, STAT1 binding, conformational changes, and transcriptional regulation, which mainly inhibits proliferation and/or promotes apoptosis (2, 3). Mouse IFN‑ gamma  R2 cDNA encodes 332 amino acids (aa), including a signal sequence (aa 1‑27), an extracellular region (ECD, aa 28‑243) with two fibronectin type III domains, a transmembrane sequence (aa 244‑264) and a cytoplasmic tail (aa 265-332) (1, 2). Within the ECD, mouse IFN‑ gamma  R2 shares 80% aa sequence identity with rat IFN‑ gamma  R2, and 49‑55% with human, canine, porcine and bovine IFN‑ gamma  R2. IFN‑ gamma  R1 and R2 must be from the same species for receptor complexes to be active, and human IFN‑ gamma is not active on the mouse IFN‑ gamma receptor complex (1, 2). IFN‑ gamma  R1 is essential for ligand binding and is more constitutively expressed, while IFN‑ gamma  R2 is essential for signaling, and its more limited expression controls cell response to IFN‑ gamma (2, 3). For example, mouse T cell IFN‑ gamma  R2 is down‑regulated during differentiation to subtypes such as Th1 which produce IFN‑ gamma . (3, 4) This allows expansion of activated cells without growth arrest due to paracrine response to IFN‑ gamma . Following expansion, IFN‑ gamma  R2 is re‑expressed to limit the immune reaction (5). IFN‑ gamma  signaling mediates control of intracellular pathogens such as mycobacteria (3, 4, 6). In humans, deficiency of IFN‑ gamma  R2 or other IFN‑ gamma pathway molecules causes the MSMD (mendelian susceptibility to mycobacterial diseases) syndrome (6‑8).

  1. Hemmi, S. et al. (1994) Cell 76:803.
  2. Krause, C.D. et al. (2006) Cell Res. 16:55.
  3. Haring, J. S. et al. (2005) J. Immunol. 174:6791.
  4. Tau, G.Z. et al. (2000) J. Exp. Med. 192:977.
  5. Foulds, K.E. et al. (2008) J. Immunol. 180:842.
  6. Rosenzweig, S.D. et al. (2004) J. Immunol. 173:4000.
  7. Filipe-Santos. O. et al. (2006) Semin. Immunol. 18:347.
  8. Zhang, S-Y. et al. (2008) Immunol. Rev. 226:29.

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Bioinformatics

Gene Symbol Ifngr2
Uniprot