Recombinant Mouse ICAM-2/CD102 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse ICAM-2/CD102 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. Recombinant Mouse ICAM‑2/CD102 Fc Chimera immobilized at 12.5 µg/mL (100 µL/well) will induce >65% cell adhesion.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived mouse ICAM-2/CD102 protein
  

  Mouse ICAM-2 (Ser20-Gln222) Accession # P35330	DIEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: P35330
• N-terminal Sequence: Ser20
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Icam2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 49.5 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 75-80 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris, Citric Acid and NaCl.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 0.4 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse ICAM-2/CD102 Fc Chimera Protein, CF

• CD102 antigen
• CD102
• ICAM2
• ICAM-2
• intercellular adhesion molecule 2

Background

Intercellular Adhesion Molecule-2 (ICAM-2, CD102), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). ICAM-2 is constitutively expressed at high levels on vascular endothelial cells and lymphohematopoietic cells. ICAM-2 mediated adhesion has been shown to provide a co-stimulatory signal for T cell aggregation, NK cytotoxicity and NK cell migration. The mouse and human ICAM-2 proteins share 60% amino acid identity. Human LFA-1 binds equally well to human or mouse ICAM-2.

1. Somersalo, K. et al. (1995) J. Biol. Chem. 270:8629.
2. Hayflick, J. et al. (1998) Immunologic Res. 17:313.
3. Xu, H. (1992) J. Immunol. 149:2650.

Publications for ICAM-2/CD102 (774-M2)(3)

Publications using 774-M2

• S Paetau, T Rolova, L Ning, CG Gahmberg Neuronal ICAM-5 Inhibits Microglia Adhesion and Phagocytosis and Promotes an Anti-inflammatory Response in LPS Stimulated Microglia Front Mol Neurosci, 2017-12-22;10(0):431. 2017-12-22 [PMID: 29311819] (Bioassay, Mouse)
• Halai , Krishma, Whiteford , James, Ma , Bin, Nourshargh , Sussan, Woodfin , Abigail ICAM-2 facilitates luminal interactions between neutrophils and endothelial cells in vivo. J Cell Sci, 2013-12-06;127(0):620-9. 2013-12-06 [PMID: 24317296] (Bioassay, Mouse)
• Furutani Y, Kawasaki M, Matsuno H, Mitsui S, Mori K, Yoshihara Y Vitronectin induces phosphorylation of ezrin/radixin/moesin actin-binding proteins through binding to its novel neuronal receptor telencephalin. J Biol Chem, 2012-09-27;287(46):39041-9. 2012-09-27 [PMID: 23019340] (Surface Plasmon Resonance)

Bioinformatics

• Gene Symbol: Icam2
• Uniprot:
  • P35330()