Recombinant Mouse Galectin-1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Galectin-1 Protein, CF Summary

• Details of Functionality: Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385. The ED 50 for this effect is 1-5 µg/mL.
• Source: E. coli-derived mouse Galectin-1 protein Ala2-Glu135
• Accession #: P16045
• N-terminal Sequence: Ala2
• Protein/Peptide Type: Recombinant Proteins
• Gene: Lgals1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Galectin-1 Protein, CF

• 14 kDa laminin-binding protein
• 14 kDa lectin
• Beta-galactoside-binding lectin L-14-I
• BHL
• DKFZp686E23103
• GAL1
• gal-1
• Galaptin
• galectin 1
• Galectin1
• Galectin-1
• GBP
• HBL
• HLBP14
• HPL
• L-14
• Lactose-binding lectin 1
• Lectin galactoside-binding soluble 1
• lectin, galactoside-binding, soluble, 1
• LGALS1
• Putative MAPK-activating protein PM12
• S-Lac lectin 1

Background

Galectin-1, gene name LGALS1 (lectin, galactoside-binding, soluble 1), is a 135 amino acid (aa), 14 kDa, pleiotropic, non-glycosylated, monomeric or homodimeric carbohydrate-binding protein of the prototype galectin family (1-3). Galectins lack a classical signal peptide and can be localized to the cytosolic compartments, or secreted via non-classical pathways (1). Secreted Galectin-1 has immunosuppressive and anti-inflammatory properties and suppresses acute and chronic inflammation and autoimmunity. It contributes to negative selection of developing T cells, immunosuppression by regulatory T cells, resolution of the inflammatory response, and inhibition of immune cell migration, inflammatory cytokine production, and mast cell degranulation (1, 2, 4-6). Galectin-1 preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, integrins alpha 4 beta 1 alpha 5 beta 1 and alpha 4 beta 7 and ganglioside GM1 (2, 3). It is produced in a variety of tissues by cells that include endothelial cells, connective tissue fibroblasts, thymic stromal cells, tumor cells, muscle cells, platelets, regulatory T cells, and activated tissue macrophages, B cells, T cells and dendritic cells (2, 3, 6-11). Most of this expression is cytosolic. Mouse Galectin-1 shares 88% aa sequence identity with human, 96% with rat, and 84% with equine, ovine, bovine and porcine Galectin-1. Endothelial cell surface expression, including tumor endothelial cells, is greatly increased by cell activation (9). Galectin-1 is highly expressed at the maternal-fetal interface and contributes to fetal immune privilege (5, 12). Its immunosuppressive properties appear to also allow tumor cells to evade immune detection (4, 5). It selectively controls T cell survival by inducing apoptosis of activated Th1 and Th17 cells, which express Galectin-1-binding glycans, while promoting Th2 cell survival where glycans are sialylated and less recognized (4, 13). It also induces apoptosis of immature thymocytes (3, 6). Galectin-1 secreted from bone marrow stromal cells aids B lymphocyte development by contributing to pre-B cell integrin adhesion and receptor signaling (3). The dimer form of Galectin-1 also induces neutrophil down-regulation by inducing cell surface exposure of phosphatidylserine that marks the cell for phagocytosis (14). Galectin-1 can also modulate cell-cell and cell-matrix interactions, and can promote either cell attachment or detachment depending on the cell type and developmental stage (1, 2).

1. Di Lella, S. et al. (2011) Biochemistry 50:7842.
2. Rabinovich, G.A. and J.M. Ilarregui (2009) Immunol. Rev. 230:144.
3. Rabinovich, G.A. and M. Vidal. (2011) Curr. Opin. Hematol. 18:443.
4. Cedeno-Laurent, F. and C.J. Dimitroff (2012) Clin. Immunol. 142:107.
5. Than, N.G. et al. (2012) Trends Endocrinol. Metab. 23:23.
6. Rubinstein, N. et al. (2004) Tissue Antigens 64:1.
7. Courad, P.O. et al. (1989) J. Biol. Chem. 264:1310.
8. Hirabayashi, J. et al. (1989) Biochim. Biophys. Acta 1008:85.
9. Thijssen, V.L. et al. (2008) Am. J. Pathol. 172:545.
10. Nio-Kobayashi, J. et al. (2009) J. Histochem. Cytochem. 57:41.
11. Dias-Baruffi, M. et al. (2010) Glycobiology 20:507.
12. Than, N.G. et al. (2008) Proc. Natl. Acad. Sci. USA 105:15819.
13. Toscano, M.A. et al. (2007) Nat. Immunol. 8:825.
14. Stowell, S.R. et al. (2009) J. Biol. Chem. 284:4989.

Publications for Galectin-1 (1245-GA/CF)(13)

Publications using 1245-GA/CF

• Mendoza, M;Ballesteros, A;Rendon-Correa, E;Tonk, R;Warren, J;Snow, AL;Stowell, SR;Blois, SM;Dveksler, G; Modulation of galectin-9 mediated responses in monocytes and T-cells by pregnancy-specific glycoprotein 1 The Journal of biological chemistry 2024-08-07 [PMID: 39121996] (Bioassay, Mouse)
• W Han, EM Mei beta ner, S Neunteibl, M Günther, J Kahnt, A Dolga, C Xie, N Plesnila, C Zhu, K Blomgren, C Culmsee Dying transplanted neural stem cells mediate survival bystander effects in the injured brain Cell Death & Disease, 2023-03-01;14(3):173. 2023-03-01 [PMID: 36854658] (Bioassay, Mouse)
• LG Morosi, AM Cutine, AJ Cagnoni, MN Manselle-C, DO Croci, JP Merlo, RM Morales, M May, JM Pérez-Sáez, MR Girotti, SP Méndez-Hue, B Pucci, AH Gil, SP Huernos, GH Docena, AM Sambuelli, MA Toscano, GA Rabinovich, KV Mariño Control of intestinal inflammation by glycosylation-dependent lectin-driven immunoregulatory circuits Science Advances, 2021-06-18;7(25):. 2021-06-18 [PMID: 34144987] (ELISA Standard, Mouse)
• JY Ge, YW Zheng, T Tsuchida, K Furuya, H Isoda, H Taniguchi, N Ohkohchi, T Oda Hepatic stellate cells contribute to liver regeneration through galectins in hepatic stem cell niche Stem Cell Res Ther, 2020-09-29;11(1):425. 2020-09-29 [PMID: 32993816] (Bioassay, Mouse)
• JM Lykken, M Horikawa, V Minard-Col, M Kamata, T Miyagaki, JC Poe, TF Tedder Galectin-1 drives lymphoma CD20 immunotherapy resistance: validation of a preclinical system to identify resistance mechanisms Blood, 2016-02-17;127(15):1886-95. 2016-02-17 [PMID: 26888257] (Bioassay, Mouse)
• Madireddi S, Eun S, Lee S, Nemcovicova I, Mehta A, Zajonc D, Nishi N, Niki T, Hirashima M, Croft M Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies. J Exp Med, 2014-06-23;211(7):1433-48. 2014-06-23 [PMID: 24958847] (Bioassay, Mouse)
• Lim M, Ahn J, Yi J, Kim M, Son A, Lee S, Lim D, Kim S, Kang M, Han Y, Song J Induction of galectin-1 by TGF-beta1 accelerates fibrosis through enhancing nuclear retention of Smad2. Exp Cell Res, 2014-06-11;326(1):125-35. 2014-06-11 [PMID: 24928277] (Bioassay, Mouse)
• Qu WS, Wang YH, Ma JF, Tian DS, Zhang Q, Pan DJ, Yu ZY, Xie MJ, Wang JP, Wang W Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia. J. Neurochem., 2010-12-02;116(2):217-26. 2010-12-02 [PMID: 21054390] (Bioassay, In Vivo, Rat)
• Cedeno-Laurent F, Barthel SR, Opperman MJ, Lee DM, Clark RA, Dimitroff CJ Development of a nascent galectin-1 chimeric molecule for studying the role of leukocyte galectin-1 ligands and immune disease modulation. J. Immunol., 2010-09-15;185(8):4659-72. 2010-09-15 [PMID: 20844192] (Bioassay, Mouse)
• Tsai CM, Chiu YK, Hsu TL, Lin IY, Hsieh SL, Lin KI Galectin-1 promotes immunoglobulin production during plasma cell differentiation. J. Immunol., 2008-10-01;181(7):4570-9. 2008-10-01 [PMID: 18802059] (Bioassay, Mouse)
• Plachta N, Annaheim C, Bissiere S, Lin S, Ruegg M, Hoving S, Muller D, Poirier F, Bibel M, Barde YA Identification of a lectin causing the degeneration of neuronal processes using engineered embryonic stem cells. Nat. Neurosci., 2007-05-07;10(6):712-9. 2007-05-07 [PMID: 17486104] (Bioassay, Mouse)
• Silva-Monteiro E, Reis Lorenzato L, Kenji Nihei O, Junqueira M, Rabinovich GA, Hsu DK, Liu FT, Savino W, Chammas R, Villa-Verde DM Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection. Am. J. Pathol., 2007-02-01;170(2):546-56. 2007-02-01 [PMID: 17255323] (Bioassay, Mouse)
• Endharti AT, Zhou YW, Nakashima I, Suzuki H Galectin-1 supports survival of naive T cells without promoting cell proliferation. Eur. J. Immunol., 2005-01-01;35(1):86-97. 2005-01-01 [PMID: 15593120] (Bioassay, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Lgals1
• Uniprot:
  • P16045()