Recombinant Mouse G-CSF Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse G-CSF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. Shirafuji, N.et al. (1989) Exp. Hematol. 17:116. The ED50 for this effect is 10-60 pg/mL.
Source
E. coli-derived mouse G-CSF protein
Val31-Ala208, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Csf3
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
414-CS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Citric Acid and Tween® 20 with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse G-CSF Protein

  • C17orf33
  • chromosome 17 open reading frame 33
  • colony stimulating factor 3 (granulocyte)
  • CSF3
  • CSF3OS
  • Filgrastim
  • GCSF
  • G-CSF
  • GCSFlenograstim
  • granulocyte colony-stimulating factor
  • Lenograstim
  • MGC45931
  • pluripoietin

Background

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.

The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.

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Publications for G-CSF (414-CS)(17)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 3 applications: Bioassay, ELISA Standard, In Vivo.


Filter By Application
Bioassay
(12)
ELISA Standard
(1)
In Vivo
(4)
All Applications
Filter By Species
Human
(2)
Mouse
(14)
N/A
(1)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using 414-CS Applications Species
M Naranjo-Go, J Lambour, M Piechaczyk, M Pelegrin Neutrophils are essential for induction of vaccine-like effects by antiviral monoclonal antibody immunotherapies JCI Insight, 2018;3(9):. 2018 [PMID: 29720574] (Bioassay, Mouse) Bioassay Mouse
A Itoh-Nakad, M Matsumoto, H Kato, J Sasaki, Y Uehara, Y Sato, R Ebina-Shib, M Morooka, R Funayama, K Nakayama, K Ochiai, A Muto, K Igarashi A Bach2-Cebp Gene Regulatory Network for the Commitment of Multipotent Hematopoietic Progenitors Cell Rep, 2017;18(10):2401-2414. 2017 [PMID: 28273455] (Bioassay, Mouse) Bioassay Mouse
Arna E Andrews Therapeutic Targeting of the G-CSF Receptor Reduces Neutrophil Trafficking and Joint Inflammation in Antibody-Mediated Inflammatory Arthritis J. Immunol., 2016;0(0):. 2016 [PMID: 27807194] (Bioassay, Mouse) Bioassay Mouse
Luyten A, Zang C, Liu X, Shivdasani R Active enhancers are delineated de novo during hematopoiesis, with limited lineage fidelity among specified primary blood cells. Genes Dev, 2014;28(16):1827-39. 2014 [PMID: 25128499] (Bioassay, Mouse) Bioassay Mouse
Doi Y, Takeuchi H, Mizoguchi H, Fukumoto K, Horiuchi H, Jin S, Kawanokuchi J, Parajuli B, Sonobe Y, Mizuno T, Suzumura A Granulocyte-colony stimulating factor attenuates oligomeric amyloid beta neurotoxicity by activation of neprilysin. PLoS ONE, 2014;9(7):e103458. 2014 [PMID: 25062013] (Bioassay, Mouse) Bioassay Mouse
Gardner J, Noel J, Nikolaidis N, Karns R, Aronow B, Ogle C, McCormack F G-CSF drives a posttraumatic immune program that protects the host from infection. J Immunol, 2014;192(5):2405-17. 2014 [PMID: 24470495] (In Vivo, Mouse) In Vivo Mouse
Fujihara Y, Takato T, Hoshi K Macrophage-inducing FasL on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration. Stem Cells, 2014;32(5):1208-19. 2014 [PMID: 24446149] (Bioassay, Human) Bioassay Human
Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M Identification of transcription factors for lineage-specific ESC differentiation. Stem Cell Reports, 2013;1(6):545-59. 2013 [PMID: 24371809] (Bioassay, Mouse) Bioassay Mouse
Nacionales D, Gentile L, Vanzant E, Lopez M, Cuenca A, Cuenca A, Ungaro R, Li Y, Baslanti T, Bihorac A, Moore F, Baker H, Leeuwenburgh C, Moldawer L, Efron P Aged mice are unable to mount an effective myeloid response to sepsis. J Immunol, 2014;192(2):612-22. 2014 [PMID: 24337739] (Bioassay, Mouse) Bioassay Mouse
A soluble form of LMIR5/CD300b amplifies lipopolysaccharide-induced lethal inflammation in sepsis. J. Immunol., 2012;189(4):1773-9. 2012 [PMID: 22772446] (Bioassay, Mouse) Bioassay Mouse
Show All 17 Publications.

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Bioinformatics

Gene Symbol Csf3
Entrez
Uniprot