Recombinant Mouse G-CSF Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse G-CSF Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. Shirafuji, N.et al. (1989) Exp. Hematol. 17:116. The ED₅₀ for this effect is 10-60 pg/mL.
• Source: E. coli-derived mouse G-CSF protein
  Val31-Ala208, with an N-terminal Met
• Accession #: P09920
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Csf3
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Citric Acid and Tween® 20 with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse G-CSF Protein

• C17orf33
• chromosome 17 open reading frame 33
• colony stimulating factor 3 (granulocyte)
• CSF3
• CSF3OS
• Filgrastim
• GCSF
• G-CSF
• GCSFlenograstim
• granulocyte colony-stimulating factor
• Lenograstim
• MGC45931
• Pluripoietin

Background

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma . Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.

The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the G_o period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.

Publications for G-CSF (414-CS)(21)

Publications using 414-CS

• Hong, NE;Chaplin, A;Di, L;Ravodina, A;Bevan, GH;Gao, H;Asase, C;Gangwar, RS;Cameron, MJ;Mignery, M;Cherepanova, O;Finn, AV;Nayak, L;Pieper, AA;Maiseyeu, A; Nanoparticle-based itaconate treatment recapitulates low-cholesterol/low-fat diet-induced atherosclerotic plaque resolution Cell reports 2024-10-27 [PMID: 39466775] (Bioassay, Mouse)
• Zewdie, EY;Edwards, GM;Hunter, DM;Earp, HS;Holtzhausen, A; MerTK Induces Dysfunctional Dendritic Cells by Metabolic Reprogramming Cancer immunology research 2024-07-08 [PMID: 38976507] (Bioassay, Mouse)
• Umene, K;Nagamune, T;Kawahara, M; Phenotypic screening of signaling motifs that efficiently induce cell proliferation Scientific reports 2023-09-20 [PMID: 37730760] (Bioassay, Mouse)
• K Maruyama, H Kidoya, N Takemura, E Sugisawa, O Takeuchi, T Kondo, MMA Eid, H Tanaka, MM Martino, N Takakura, Y Takayama, S Akira, A Vandenbon, Y Kumagai Zinc Finger Protein St18 Protects against Septic Death by Inhibiting VEGF-A from Macrophages Cell Rep, 2020-07-14;32(2):107906. 2020-07-14 [PMID: 32668247] (Bioassay, Mouse)
• M Naranjo-Go, J Lambour, M Piechaczyk, M Pelegrin Neutrophils are essential for induction of vaccine-like effects by antiviral monoclonal antibody immunotherapies JCI Insight, 2018-05-03;3(9):. 2018-05-03 [PMID: 29720574] (Bioassay, Mouse)
• A Itoh-Nakad, M Matsumoto, H Kato, J Sasaki, Y Uehara, Y Sato, R Ebina-Shib, M Morooka, R Funayama, K Nakayama, K Ochiai, A Muto, K Igarashi A Bach2-Cebp Gene Regulatory Network for the Commitment of Multipotent Hematopoietic Progenitors Cell Rep, 2017-03-07;18(10):2401-2414. 2017-03-07 [PMID: 28273455] (Bioassay, Mouse)
• Arna E Andrews Therapeutic Targeting of the G-CSF Receptor Reduces Neutrophil Trafficking and Joint Inflammation in Antibody-Mediated Inflammatory Arthritis J. Immunol., 2016-11-02;0(0):. 2016-11-02 [PMID: 27807194] (Bioassay, Mouse)
• Luyten A, Zang C, Liu X, Shivdasani R Active enhancers are delineated de novo during hematopoiesis, with limited lineage fidelity among specified primary blood cells. Genes Dev, 2014-08-15;28(16):1827-39. 2014-08-15 [PMID: 25128499] (Bioassay, Mouse)
• Doi Y, Takeuchi H, Mizoguchi H, Fukumoto K, Horiuchi H, Jin S, Kawanokuchi J, Parajuli B, Sonobe Y, Mizuno T, Suzumura A Granulocyte-colony stimulating factor attenuates oligomeric amyloid beta neurotoxicity by activation of neprilysin. PLoS ONE, 2014-07-25;9(7):e103458. 2014-07-25 [PMID: 25062013] (Bioassay, ICC, Mouse)
• Gardner J, Noel J, Nikolaidis N, Karns R, Aronow B, Ogle C, McCormack F G-CSF drives a posttraumatic immune program that protects the host from infection. J Immunol, 2014-01-27;192(5):2405-17. 2014-01-27 [PMID: 24470495] (In Vivo, Mouse)
• Fujihara Y, Takato T, Hoshi K Macrophage-inducing FasL on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration. Stem Cells, 2014-05-01;32(5):1208-19. 2014-05-01 [PMID: 24446149] (Bioassay, Human, Mouse)
• Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M Identification of transcription factors for lineage-specific ESC differentiation. Stem Cell Reports, 2013-11-27;1(6):545-59. 2013-11-27 [PMID: 24371809] (Bioassay, Mouse)
• Nacionales D, Gentile L, Vanzant E, Lopez M, Cuenca A, Cuenca A, Ungaro R, Li Y, Baslanti T, Bihorac A, Moore F, Baker H, Leeuwenburgh C, Moldawer L, Efron P Aged mice are unable to mount an effective myeloid response to sepsis. J Immunol, 2013-12-13;192(2):612-22. 2013-12-13 [PMID: 24337739] (Bioassay, Mouse)
• A soluble form of LMIR5/CD300b amplifies lipopolysaccharide-induced lethal inflammation in sepsis. J. Immunol., 2012-07-06;189(4):1773-9. 2012-07-06 [PMID: 22772446] (Bioassay, Mouse)
• Cook BD, Liu S, Evans T Smad1 signaling restricts hematopoietic potential after promoting hemangioblast commitment. Blood, 2011-04-22;117(24):6489-97. 2011-04-22 [PMID: 21515822] (Bioassay, Mouse)
• Shimoji K, Yuasa S, Onizuka T, Hattori F, Tanaka T, Hara M, Ohno Y, Chen H, Egasgira T, Seki T, Yae K, Koshimizu U, Ogawa S, Fukuda K G-CSF promotes the proliferation of developing cardiomyocytes in vivo and in derivation from ESCs and iPSCs. Cell Stem Cell, 2010-03-05;6(3):227-37. 2010-03-05 [PMID: 20207226] (Bioassay, In Vivo, Mouse)
• Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N Bv8 regulates myeloid-cell-dependent tumour angiogenesis. Nature, 2007-12-06;450(7171):825-31. 2007-12-06 [PMID: 18064003] (Bioassay, In Vivo, Mouse)
• Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Stem Cells, 2007-02-01;25(2):490-9. 2007-02-01 [PMID: 17284653] (Bioassay, Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque))
• Kim SY, Nguyen AD, Gao JL, Murphy PM, Mansfield BC, Chou JY Bone marrow-derived cells require a functional glucose 6-phosphate transporter for normal myeloid functions. J. Biol. Chem., 2006-08-04;281(39):28794-801. 2006-08-04 [PMID: 16891306] (Bioassay, Mouse)
• Heissig B, Hattori K, Dias S, Friedrich M, Ferris B, Hackett NR, Crystal RG, Besmer P, Lyden D, Moore MA, Werb Z, Rafii S Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand. Cell, 2002-05-31;109(5):625-37. 2002-05-31 [PMID: 12062105] (In Vivo, Mouse)
• Ye P, Rodriguez FH, Kanaly S, Stocking KL, Schurr J, Schwarzenberger P, Oliver P, Huang W, Zhang P, Zhang J, Shellito JE, Bagby GJ, Nelson S, Charrier K, Peschon JJ, Kolls JK Requirement of interleukin 17 receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor expression, neutrophil recruitment, and host defense. J. Exp. Med., 2001-08-20;194(4):519-27. 2001-08-20 [PMID: 11514607] (ELISA (Standard))

Bioinformatics

• Gene Symbol: Csf3
• Uniprot:
  • P09920()