Recombinant Mouse Frizzled-9 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse Frizzled-9 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a funtional ELISA with an estimated Kd <10 nM.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Frizzled-9 protein
Mouse Frizzled-9
(Leu24-Asp186)
Accession # Q9R216
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Leu24
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Fzd9
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
45 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
58-66 kDa, reducing conditions
Publications
Read Publication using
2440-FZ in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Frizzled-9 Fc Chimera Protein, CF

  • CD349 antigen
  • CD349
  • frizzled (Drosophila) homolog 9
  • frizzled homolog 9 (Drosophila)
  • Frizzled9
  • Frizzled-9
  • fz-9
  • FZD3fzE6
  • FZD9
  • FzE6
  • hFz9

Background

Frizzled-9 (Fzd9, previously called Fzd3) belongs to the Frizzled family of seven-transmembrane glycoprotein receptors, which are signaling receptors for the family of secreted Wnt ligands (1-3). Mature mouse Frizzled-9 consists of a 207 amino acid (aa) N-terminal extracellular domain (ECD), the 7TM region, and a 62 aa C-terminal cytoplasmic domain with a PDZ binding motif. The ECD includes a cysteine-rich region that binds Wnts and is highly conserved among Frizzleds. Within aa 23-186 of the N-terminal ECD, mouse Frizzled-9 shares 97% and 100% aa identity with human and rat Frizzled-9, respectively. The human Frizzled-9 gene maps to a large region that is deleted in Williams-Beuren syndrome, a developmental disorder affecting personality and cognition (2). Frizzled-9 mRNA is expressed in the central nervous system and myotomes during development, and it persists in the hippocampus throughout life (2-4). Adults also show expression in heart, testis, and skeletal muscle (2-4). Cellular expression is detected in proliferating neural precursors, B lymphocyte progenitors, differentiating osteoblasts, and developing myotubes of skeletal muscle (4-7). Mice lacking Frizzled-9 exhibit defects in learning, B lymphocyte development, and bone mineralization (4-6, 8). Frizzled-9 can signal through both canonical and non-canonical pathways (6, 9). Its binding to Wnt-7a promotes the activation of PPAR gamma, expression of Cadherin and Sprouty, and reversal of non-small cell lung cancer cell transformation (10-12).
  1. Dijksterhuis, J.P. et al. (2014) Br. J. Pharmacol. 171:1195.
  2. Wang, Y.K. et al. (1997) Hum. Mol. Genet. 6:465.
  3. Wang, Y.K. et al. (1999) Genomics 57:235.
  4. Zhao, C. et al. (2005) Development 132:2917.
  5. Ranheim, E.A. et al. (2005) Blood 105:2487.
  6. Albers, J. et al. (2011) J. Cell Biol. 192:1057.
  7. Aviles, E.C. et al. (2014) Front. Cell. Neurosci. 8:110.
  8. Heilmann, A. et al. (2013) PLoS One 8:e84232.
  9. Karasawa, T. et al. (2002) J. Biol. Chem. 277:37479.
  10. Winn, R.A. et al. (2005) J. Biol. Chem. 280:19625.
  11. Winn, R.A. et al. (2006) J. Biol. Chem. 281:26943.
  12. Tennis, M.A. et al. (2010) Mol. Cancer Res. 8:833.

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Publications for Frizzled-9 (2440-FZ)(1)

We have publications tested in 1 confirmed species: N/A.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol Fzd9
Uniprot