Recombinant Mouse Frizzled-9 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Frizzled-9 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a funtional ELISA with an estimated K_d <10 nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Frizzled-9 protein
  

  Mouse Frizzled-9 (Leu24-Asp186) Accession # Q9R216	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: Q9R216
• N-terminal Sequence: Leu24
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Fzd9
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 45 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 58-66 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Frizzled-9 Fc Chimera Protein, CF

• CD349 antigen
• CD349
• frizzled (Drosophila) homolog 9
• frizzled homolog 9 (Drosophila)
• Frizzled9
• Frizzled-9
• fz-9
• FZD3fzE6
• FZD9
• FzE6
• hFz9

Background

Frizzled-9 (Fzd9, previously called Fzd3) belongs to the Frizzled family of seven-transmembrane glycoprotein receptors, which are signaling receptors for the family of secreted Wnt ligands (1-3). Mature mouse Frizzled-9 consists of a 207 amino acid (aa) N-terminal extracellular domain (ECD), the 7TM region, and a 62 aa C-terminal cytoplasmic domain with a PDZ binding motif. The ECD includes a cysteine-rich region that binds Wnts and is highly conserved among Frizzleds. Within aa 23-186 of the N-terminal ECD, mouse Frizzled-9 shares 97% and 100% aa identity with human and rat Frizzled-9, respectively. The human Frizzled-9 gene maps to a large region that is deleted in Williams-Beuren syndrome, a developmental disorder affecting personality and cognition (2). Frizzled-9 mRNA is expressed in the central nervous system and myotomes during development, and it persists in the hippocampus throughout life (2-4). Adults also show expression in heart, testis, and skeletal muscle (2-4). Cellular expression is detected in proliferating neural precursors, B lymphocyte progenitors, differentiating osteoblasts, and developing myotubes of skeletal muscle (4-7). Mice lacking Frizzled-9 exhibit defects in learning, B lymphocyte development, and bone mineralization (4-6, 8). Frizzled-9 can signal through both canonical and non-canonical pathways (6, 9). Its binding to Wnt-7a promotes the activation of PPAR gamma , expression of Cadherin and Sprouty, and reversal of non-small cell lung cancer cell transformation (10-12).

1. Dijksterhuis, J.P. et al. (2014) Br. J. Pharmacol. 171:1195.
2. Wang, Y.K. et al. (1997) Hum. Mol. Genet. 6:465.
3. Wang, Y.K. et al. (1999) Genomics 57:235.
4. Zhao, C. et al. (2005) Development 132:2917.
5. Ranheim, E.A. et al. (2005) Blood 105:2487.
6. Albers, J. et al. (2011) J. Cell Biol. 192:1057.
7. Aviles, E.C. et al. (2014) Front. Cell. Neurosci. 8:110.
8. Heilmann, A. et al. (2013) PLoS One 8:e84232.
9. Karasawa, T. et al. (2002) J. Biol. Chem. 277:37479.
10. Winn, R.A. et al. (2005) J. Biol. Chem. 280:19625.
11. Winn, R.A. et al. (2006) J. Biol. Chem. 281:26943.
12. Tennis, M.A. et al. (2010) Mol. Cancer Res. 8:833.

Publications for Frizzled-9 (2440-FZ)(1)

Publication using 2440-FZ

• Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation J Biol Chem, 2016-07-11;0(0):. 2016-07-11 [PMID: 27402827] (Bioassay)

Bioinformatics

• Gene Symbol: Fzd9
• Uniprot:
  • Q9R216()