Recombinant Mouse Frizzled-8 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity, Bioactivity
• Format: Carrier-Free

Recombinant Mouse Frizzled-8 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 15-75 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN). Measured by its ability to bind biotinylated recombinant mouse Wnt-5a in a functional ELISA with an estimated K_d <1 nM.
• Source: Mouse myeloma cell line, NS0-derived mouse Frizzled-8 protein
  

  Mouse Frizzled-8 (Ala25-Pro173) & (Ala28-Pro173) Accession # Q61091	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q61091
• N-terminal Sequence: Ala25 & Ala28
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Fzd8
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
  • Bioactivity
• Theoretical MW: 43 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 59-60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Frizzled-8 Fc Chimera Protein, CF

• frizzled (Drosophila) homolog 8
• frizzled 8
• frizzled family receptor 8
• frizzled homolog 8 (Drosophila)
• Frizzled8
• Frizzled-8
• FZ-8
• FZD8
• hFZ8

Background

Frizzled-8 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors (1, 2). Frizzled proteins are thought to be G‑protein‑coupled (1). Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP‑6 acting as co‑receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance (1). Mouse Frizzled-8 cDNA encodes 685 amino acids (aa) including a 27 aa signal peptide, a 244 aa extracellular domain (ECD), the 7TM region (aa 272-603), and a C-terminal cytoplasmic domain with a PDZ binding motif (aa 604-685) (2). The ECD includes a cysteine-rich region (CRD, aa 30-151) that binds Wnts and is highly conserved among Frizzleds, and a linker region. Within aa 25-173, mouse Frizzled-8 shares 99%, 99%, 90% and 85% aa identity with human, rat, Xenopus and zebrafish Frizzled-8, respectively. It also shares 82% aa identity with human Frizzled-5. Frizzleds can form homodimers or selective hetero-oligomers with other family members, which can involve the TM regions and possibly the CRD (1, 3). During mouse development, Frizzled-8 is expressed in tissues that are important for organizing the anterior-posterior axis (4). Interactions of Frizzled-8 with several non-Wnt ligands have been identified. Interaction with Frizzled-8 and LRP-6 allows R-spondins to activate beta -catenin signaling pathways, while interaction with IGFBP-4 (insulin-like growth factor binding protein-4) or CTGF (connective tissue growth factor) inhibits Wnt signaling (5‑7). These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas (5‑10).

1. Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518.
2. Wang, Y. et al. (1996) J. Biol. Chem. 271:4468.
3. Dann, C.E. et al. (2001) Nature 412:86.
4. Lu C.C. et al. (2004) Gene Expr. Patterns 4:569.
5. Nam, J-S. et al. (2006) J. Biol. Chem. 281:13247.
6. Zhu, W. et al. (2008) Nature 454:345.
7. Mercurio, S. et al. (2004) Development 131:2137.
8. Chesnutt, C. et al. (2004) Dev. Biol. 274:334.
9. Yamashita, J.K. et al. (2005) FASEB J. 19:1534.
10. DeAlmeida, V.I. et al. (2007) Cancer Res. 67:5371.

Publications for Frizzled-8 (112-FZ)(3)

Publications using 112-FZ

• Esen E, Lee S, Wice B, Long F PTH Promotes Bone Anabolism by Stimulating Aerobic Glycolysis via IGF Signaling. J Bone Miner Res, 2015-07-14;30(11):1959-68. 2015-07-14 [PMID: 25990470] (Bioassay, Mouse)
• Lindsley RC, Gill JG, Kyba M, Murphy TL, Murphy KM Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm. Development, 2006-08-30;133(19):3787-96. 2006-08-30 [PMID: 16943279] (Bioassay, Mouse)
• Viti J, Gulacsi A, Lillien L Wnt regulation of progenitor maturation in the cortex depends on Shh or fibroblast growth factor 2. J. Neurosci., 2003-07-02;23(13):5919-27. 2003-07-02 [PMID: 12843296] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Fzd8
• Uniprot:
  • Q61091()