Recombinant Mouse Ephrin-B1 Fc Chimera Protein, CF


There are currently no images for Ephrin-B1 (473-EB).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity MuSpecies Glossary
Applications Binding Activity

Order Details

Recombinant Mouse Ephrin-B1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse EphB3 Fc Chimera at 2 µg/mL (100 µL/well) can bind Recombinant Mouse Ephrin-B1 Fc Chimera with a linear range of 0.01-0.5 ng/mL.
Mouse myeloma cell line, NS0-derived mouse Ephrin-B1 protein
Mouse Ephrin-B1
Accession # AAA53231
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Binding Activity
Theoretical MW
49.2 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
60 kDa, reducing conditions
Read Publications using
473-EB in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Ephrin-B1 Fc Chimera Protein, CF

  • Cek5-L
  • EFL3
  • EFL-3
  • EFNB1
  • ELK ligand
  • ELK-L
  • EphrinB1
  • Ephrin-B1
  • LERK-2
  • STRA-1


Ephrin-B1, also known as Elk Ligand, LERK2, and Eplg2, is an approximately 45 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains (ECD) of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B1 preferentially interacts with receptors in the EphB family. The binding of Ephrin-B1 to EphB proteins also triggers reverse signaling through Ephrin-B1 (1, 2). Mature mouse Ephrin-B1 consists of a 212 amino acid (aa) ECD, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain (3, 4). Within the ECD, mouse Ephrin-B1 shares 94% and 98% aa sequence identity with human and rat Ephrin-B1, respectively. Ligation by EphB2 enhances shedding of a 35 kDa fragment of the Ephrin-B1 ECD (5). The residual membrane-bound portion is then cleaved by gamma-secretase to release the intracellular domain (6). Ephrin-B1 also associates in cis with Claudin-1, -4, and -5 (7, 8). It is expressed on glomerular podocyte slit diaphragms, developing thymocytes, peripheral T cells, monocytes, macrophages, vascular endothelial cells, cardiomyocytes, osteoclasts, and luteinizing granulosa cells in the ovary (8-13). In the developing nervous system, Ephrin-B1 plays a role in cellular migration, axon guidance, and presynaptic development (14-16). It also regulates developing thymocyte survival, monocyte migration, osteoclast differentiation and function, cardiac muscle morphogenesis, and tumorigenesis (5, 8, 10-12). Ephrin-B1 is up-regulated on reactive astrocytes and on macrophages and T cells found in atherosclerotic plaques (11, 17).
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Shao, H. et al. (1994) J. Biol. Chem. 269:26606.
  4. Fletcher, F.A. et al. (1994) Genomics 24:127.
  5. Tanaka, M. et al. (2007) J. Cell Sci. 120:2179.
  6. Tomita, T. et al. (2006) Mol. Neurodegen. 1:2.
  7. Tanaka, M. et al. (2005) EMBO J. 24:3700.
  8. Genet, G. et al. (2012) Circ. Res. 110:688.
  9. Hashimoto, T. et al. (2007) Kidney Int. 72:954.
  10. Yu, G. et al. (2006) J. Biol. Chem. 281:10222.
  11. Sakamoto, A. et al. (2008) Clin. Sci. 114:643.
  12. Cheng, S. et al. (2012) PLoS ONE 7:e32887.
  13. Egawa, M. et al. (2003) J. Clin. Endocrinol. Metab. 88:4384.
  14. Davy, A. et al. (2004) Genes Dev. 18:572.
  15. Bush, J.O. and P. Soriano (2009) Genes Dev. 23:1586.
  16. McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. USA 106:20487.
  17. Wang, Y. et al. (2005) Eur. J. Neurosci. 21:2336.

Customers Who Viewed This Item Also Viewed...

Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
Species: Mu
Applications: Bind
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
Species: Rt
Applications: Bind
Species: Mu
Applications: Bind
Species: Hu
Applications: WB
Species: Mu
Applications: CyTOF-ready, Flow, ICC, WB
Species: Hu
Applications: IHC, IHC-P
Species: Mu
Applications: BA
Species: Mu
Applications: IHC, WB
Species: Hu
Applications: Bind
Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
Species: Hu
Applications: CyTOF-ready, Flow, IHC, KO, WB
Species: Hu
Applications: ICC, IHC, WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
Species: Hu, Mu
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, KD, WB
Species: Hu
Applications: Bind
Species: Ca, Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB

Publications for Ephrin-B1 (473-EB)(26)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Chicken, Virus - Henipavirus, Xenopus, Zebrafish.

We have publications tested in 7 applications: Bioassay, Control, Coprecipitation Assay, Enzyme Assay, IHC, IHC-Fr, In Vivo.

Filter By Application
Coprecipitation Assay
Enzyme Assay
In Vivo
All Applications
Filter By Species
Virus - Henipavirus
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using 473-EB Applications Species
Chang, C;Banerjee, SL;Park, SS;Zhang, X;Cotnoir-White, D;Desbois, M;Grill, B;Kania, A; Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function bioRxiv : the preprint server for biology 2023-08-29 [PMID: 37693478] (Bioassay, Human, Mouse) Bioassay Human, Mouse
S Kohara, K Ogawa Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells Biomedicines, 2022-12-12;10(12):. 2022-12-12 [PMID: 36551990] (Bioassay, Mouse) Bioassay Mouse
ED Laing, CK Navaratnar, S Cheliout D, SR Petzing, Y Xu, SL Sterling, GA Marsh, LF Wang, M Amaya, DB Nikolov, R Cattaneo, CC Broder, K Xu Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus Proc. Natl. Acad. Sci. U.S.A., 2019-09-23;0(0):. 2019-09-23 [PMID: 31548390] (Coprecipitation Assay, Virus - Henipavirus) Coprecipitation Assay Virus - Henipavirus
MA Fawal, T Jungas, A Kischel, C Audouard, JS Iacovoni, A Davy Cross Talk between One-Carbon Metabolism, Eph Signaling, and Histone Methylation Promotes Neural Stem Cell Differentiation Cell Rep, 2018-06-05;23(10):2864-2873.e7. 2018-06-05 [PMID: 29874574] (Bioassay, Human) Bioassay Human
AK O'Neill, AA Kindberg, TK Niethamer, AR Larson, HH Ho, ME Greenberg, JO Bush Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation J. Cell Biol., 2016-10-17;215(2):217-229. 2016-10-17 [PMID: 27810913] (Control) Control
Mimche P, Brady L, Keeton S, Fenne D, King T, Quicke K, Hudson L, Lamb T Expression of the Receptor Tyrosine Kinase EphB2 on Dendritic Cells Is Modulated by Toll-Like Receptor Ligation but Is Not Required for T Cell Activation. PLoS ONE, 2015-09-25;10(9):e0138835. 2015-09-25 [PMID: 26407069] (Bioassay, Mouse) Bioassay Mouse
Zhang , Gu, Brady , John, Liang , Wei-Chin, Wu , Yan, Henkemeyer , Mark, Yan , Minhong EphB4 forward signalling regulates lymphatic valve development. Nat Commun, 2015-04-13;6(0):6625. 2015-04-13 [PMID: 25865237] (Enzyme Assay, Mouse) Enzyme Assay Mouse
Soskis , Michael, Ho , Hsin-Yi, Bloodgood , Brenda L, Robichaux , Michael, Malik , Athar N, Ataman , Bulent, Rubin , Alex A, Zieg , Janine, Zhang , Chao, Shokat , Kevan M, Sharma , Nikhil, Cowan , Christop, Greenberg , Michael A chemical genetic approach reveals distinct EphB signaling mechanisms during brain development. Nat Neurosci, 2012-11-11;15(12):1645-54. 2012-11-11 [PMID: 23143520] (Bioassay, Mouse) Bioassay Mouse
Yu L, Zhou X, Yu J, Huang H, Jiang L, Zhang F, Cao J, Yan M PI3K contributed to modulation of spinal nociceptive information related to ephrinBs/EphBs. PLoS ONE, 2012-08-03;7(8):e40930. 2012-08-03 [PMID: 22879882] (In Vivo, Mouse) In Vivo Mouse
Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition. Eur. J. Immunol., 2012-05-23;42(6):1562-72. 2012-05-23 [PMID: 22622783] (Bioassay, Mouse) Bioassay Mouse
Show All 26 Publications.

Reviews for Ephrin-B1 (473-EB) (0)

There are no reviews for Ephrin-B1 (473-EB). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Ephrin-B1 (473-EB) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Mouse Ephrin-B1 Fc Chimera Protein, CF and receive a gift card or discount.


Gene Symbol Efnb1