Recombinant Mouse Ephrin-B1 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Mouse Ephrin-B1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse EphB3 Fc Chimera at 2 µg/mL (100 µL/well) can bind Recombinant Mouse Ephrin-B1 Fc Chimera with a linear range of 0.01-0.5 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Ephrin-B1 protein
Mouse Ephrin-B1
(Lys30-Ser229)
Accession # AAA53231
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Lys30
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Efnb1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
49.2 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60 kDa, reducing conditions
Publications
Read Publications using
473-EB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Ephrin-B1 Fc Chimera Protein, CF

  • Cek5-L
  • EFL-3
  • EFNB1
  • ELK-L
  • EphrinB1
  • Ephrin-B1
  • LERK-2
  • STRA-1

Background

Ephrin-B1, also known as Elk Ligand, LERK2, and Eplg2, is an approximately 45 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains (ECD) of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B1 preferentially interacts with receptors in the EphB family. The binding of Ephrin-B1 to EphB proteins also triggers reverse signaling through Ephrin-B1 (1, 2). Mature mouse Ephrin-B1 consists of a 212 amino acid (aa) ECD, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain (3, 4). Within the ECD, mouse Ephrin-B1 shares 94% and 98% aa sequence identity with human and rat Ephrin-B1, respectively. Ligation by EphB2 enhances shedding of a 35 kDa fragment of the Ephrin-B1 ECD (5). The residual membrane-bound portion is then cleaved by gamma-secretase to release the intracellular domain (6). Ephrin-B1 also associates in cis with Claudin-1, -4, and -5 (7, 8). It is expressed on glomerular podocyte slit diaphragms, developing thymocytes, peripheral T cells, monocytes, macrophages, vascular endothelial cells, cardiomyocytes, osteoclasts, and luteinizing granulosa cells in the ovary (8-13). In the developing nervous system, Ephrin-B1 plays a role in cellular migration, axon guidance, and presynaptic development (14-16). It also regulates developing thymocyte survival, monocyte migration, osteoclast differentiation and function, cardiac muscle morphogenesis, and tumorigenesis (5, 8, 10-12). Ephrin-B1 is up-regulated on reactive astrocytes and on macrophages and T cells found in atherosclerotic plaques (11, 17).
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Shao, H. et al. (1994) J. Biol. Chem. 269:26606.
  4. Fletcher, F.A. et al. (1994) Genomics 24:127.
  5. Tanaka, M. et al. (2007) J. Cell Sci. 120:2179.
  6. Tomita, T. et al. (2006) Mol. Neurodegen. 1:2.
  7. Tanaka, M. et al. (2005) EMBO J. 24:3700.
  8. Genet, G. et al. (2012) Circ. Res. 110:688.
  9. Hashimoto, T. et al. (2007) Kidney Int. 72:954.
  10. Yu, G. et al. (2006) J. Biol. Chem. 281:10222.
  11. Sakamoto, A. et al. (2008) Clin. Sci. 114:643.
  12. Cheng, S. et al. (2012) PLoS ONE 7:e32887.
  13. Egawa, M. et al. (2003) J. Clin. Endocrinol. Metab. 88:4384.
  14. Davy, A. et al. (2004) Genes Dev. 18:572.
  15. Bush, J.O. and P. Soriano (2009) Genes Dev. 23:1586.
  16. McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. USA 106:20487.
  17. Wang, Y. et al. (2005) Eur. J. Neurosci. 21:2336.

Publications for Ephrin-B1 (473-EB)(23)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Xenopus, Zebrafish.

We have publications tested in 7 applications: Bioassay, EnzAct, IHC, IHC Fresh, IHC Frozen, In Vivo, Positivie Ctrl.


Filter By Application
Bioassay
(14)
EnzAct
(1)
IHC
(1)
IHC Fresh
(2)
IHC Frozen
(1)
In Vivo
(3)
Positivie Ctrl
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(11)
Rat
(1)
Chicken
(2)
Xenopus
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using 473-EB Applications Species
MA Fawal, T Jungas, A Kischel, C Audouard, JS Iacovoni, A Davy Cross Talk between One-Carbon Metabolism, Eph Signaling, and Histone Methylation Promotes Neural Stem Cell Differentiation Cell Rep, 2018;23(10):2864-2873.e7. 2018 [PMID: 29874574] (Bioassay, Human) Bioassay Human
AK O'Neill, AA Kindberg, TK Niethamer, AR Larson, HH Ho, ME Greenberg, JO Bush Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation J. Cell Biol., 2016;215(2):217-229. 2016 [PMID: 27810913] (Positivie Ctrl) Positivie Ctrl
Mimche P, Brady L, Keeton S, Fenne D, King T, Quicke K, Hudson L, Lamb T Expression of the Receptor Tyrosine Kinase EphB2 on Dendritic Cells Is Modulated by Toll-Like Receptor Ligation but Is Not Required for T Cell Activation. PLoS ONE, 2015;10(9):e0138835. 2015 [PMID: 26407069] (Bioassay, Mouse) Bioassay Mouse
Zhang, Gu, Brady, John, Liang, Wei-Chin, Wu, Yan, Henkemeyer, Mark, Yan, Minhong EphB4 forward signalling regulates lymphatic valve development. Nat Commun, 2015;6(0):6625. 2015 [PMID: 25865237] (EnzAct, Mouse) EnzAct Mouse
Soskis, Michael, Ho, Hsin-Yi, Bloodgood, Brenda L, Robichaux, Michael, Malik, Athar N, Ataman, Bulent, Rubin, Alex A, Zieg, Janine, Zhang, Chao, Shokat, Kevan M, Sharma, Nikhil, Cowan, Christop, Greenberg, Michael A chemical genetic approach reveals distinct EphB signaling mechanisms during brain development. Nat Neurosci, 2012;15(12):1645-54. 2012 [PMID: 23143520] (Bioassay, Mouse) Bioassay Mouse
Yu L, Zhou X, Yu J, Huang H, Jiang L, Zhang F, Cao J, Yan M PI3K contributed to modulation of spinal nociceptive information related to ephrinBs/EphBs. PLoS ONE, 2012;7(8):e40930. 2012 [PMID: 22879882] (In Vivo, Mouse) In Vivo Mouse
Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition. Eur. J. Immunol., 2012;42(6):1562-72. 2012 [PMID: 22622783] (Bioassay, Mouse) Bioassay Mouse
EphB regulates L1 phosphorylation during retinocollicular mapping. Mol. Cell. Neurosci., 2012;50(2):201-10. 2012 [PMID: 22579729] (Bioassay, Human) Bioassay Human
Maddigan A, Truitt L, Arsenault R EphB receptors trigger Akt activation and suppress Fas receptor-induced apoptosis in malignant T lymphocytes. J. Immunol., 2011;187(11):5983-94. 2011 [PMID: 22039307] (Bioassay, Human) Bioassay Human
Pittman AJ, Gaynes JA, Chien CB nev (cyfip2) is required for retinal lamination and axon guidance in the zebrafish retinotectal system. Dev. Biol., 2010;344(2):784-94. 2010 [PMID: 20537992] (IHC, Zebrafish) IHC Zebrafish
Show All 23 Publications.

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Bioinformatics

Gene Symbol Efnb1
Entrez
Uniprot