Recombinant Mouse Ephrin-B1 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse Ephrin-B1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse EphB3 Fc Chimera at 2 µg/mL (100 µL/well) can bind Recombinant Mouse Ephrin-B1 Fc Chimera with a linear range of 0.01-0.5 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Ephrin-B1 protein
  

  Mouse Ephrin-B1 (Lys30-Ser229) Accession # AAA53231	IEGRMD	Human IgG1 (Pro100-Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #: AAA53231
• N-terminal Sequence: Lys30
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Efnb1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 49.2 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Ephrin-B1 Fc Chimera Protein, CF

• Cek5-L
• EFL3
• EFL-3
• EFNB1
• ELK ligand
• ELK-L
• EphrinB1
• Ephrin-B1
• LERK-2
• STRA-1

Background

Ephrin-B1, also known as Elk Ligand, LERK2, and Eplg2, is an approximately 45 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains (ECD) of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B1 preferentially interacts with receptors in the EphB family. The binding of Ephrin-B1 to EphB proteins also triggers reverse signaling through Ephrin-B1 (1, 2). Mature mouse Ephrin-B1 consists of a 212 amino acid (aa) ECD, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain (3, 4). Within the ECD, mouse Ephrin-B1 shares 94% and 98% aa sequence identity with human and rat Ephrin-B1, respectively. Ligation by EphB2 enhances shedding of a 35 kDa fragment of the Ephrin-B1 ECD (5). The residual membrane-bound portion is then cleaved by gamma-secretase to release the intracellular domain (6). Ephrin-B1 also associates in cis with Claudin-1, -4, and -5 (7, 8). It is expressed on glomerular podocyte slit diaphragms, developing thymocytes, peripheral T cells, monocytes, macrophages, vascular endothelial cells, cardiomyocytes, osteoclasts, and luteinizing granulosa cells in the ovary (8-13). In the developing nervous system, Ephrin-B1 plays a role in cellular migration, axon guidance, and presynaptic development (14-16). It also regulates developing thymocyte survival, monocyte migration, osteoclast differentiation and function, cardiac muscle morphogenesis, and tumorigenesis (5, 8, 10-12). Ephrin-B1 is up-regulated on reactive astrocytes and on macrophages and T cells found in atherosclerotic plaques (11, 17).

1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
3. Shao, H. et al. (1994) J. Biol. Chem. 269:26606.
4. Fletcher, F.A. et al. (1994) Genomics 24:127.
5. Tanaka, M. et al. (2007) J. Cell Sci. 120:2179.
6. Tomita, T. et al. (2006) Mol. Neurodegen. 1:2.
7. Tanaka, M. et al. (2005) EMBO J. 24:3700.
8. Genet, G. et al. (2012) Circ. Res. 110:688.
9. Hashimoto, T. et al. (2007) Kidney Int. 72:954.
10. Yu, G. et al. (2006) J. Biol. Chem. 281:10222.
11. Sakamoto, A. et al. (2008) Clin. Sci. 114:643.
12. Cheng, S. et al. (2012) PLoS ONE 7:e32887.
13. Egawa, M. et al. (2003) J. Clin. Endocrinol. Metab. 88:4384.
14. Davy, A. et al. (2004) Genes Dev. 18:572.
15. Bush, J.O. and P. Soriano (2009) Genes Dev. 23:1586.
16. McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. USA 106:20487.
17. Wang, Y. et al. (2005) Eur. J. Neurosci. 21:2336.

Publications for Ephrin-B1 (473-EB)(25)

Publications using 473-EB

• S Kohara, K Ogawa Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells Biomedicines, 2022-12-12;10(12):. 2022-12-12 [PMID: 36551990] (Bioassay, Mouse)
• ED Laing, CK Navaratnar, S Cheliout D, SR Petzing, Y Xu, SL Sterling, GA Marsh, LF Wang, M Amaya, DB Nikolov, R Cattaneo, CC Broder, K Xu Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus Proc. Natl. Acad. Sci. U.S.A., 2019-09-23;0(0):. 2019-09-23 [PMID: 31548390] (Coprecipitation Assay, Virus - Henipavirus)
• MA Fawal, T Jungas, A Kischel, C Audouard, JS Iacovoni, A Davy Cross Talk between One-Carbon Metabolism, Eph Signaling, and Histone Methylation Promotes Neural Stem Cell Differentiation Cell Rep, 2018-06-05;23(10):2864-2873.e7. 2018-06-05 [PMID: 29874574] (Bioassay, Human)
• AK O'Neill, AA Kindberg, TK Niethamer, AR Larson, HH Ho, ME Greenberg, JO Bush Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation J. Cell Biol., 2016-10-17;215(2):217-229. 2016-10-17 [PMID: 27810913] (Control)
• Mimche P, Brady L, Keeton S, Fenne D, King T, Quicke K, Hudson L, Lamb T Expression of the Receptor Tyrosine Kinase EphB2 on Dendritic Cells Is Modulated by Toll-Like Receptor Ligation but Is Not Required for T Cell Activation. PLoS ONE, 2015-09-25;10(9):e0138835. 2015-09-25 [PMID: 26407069] (Bioassay, Mouse)
• Zhang , Gu, Brady , John, Liang , Wei-Chin, Wu , Yan, Henkemeyer , Mark, Yan , Minhong EphB4 forward signalling regulates lymphatic valve development. Nat Commun, 2015-04-13;6(0):6625. 2015-04-13 [PMID: 25865237] (Enzyme Assay, Mouse)
• Soskis , Michael, Ho , Hsin-Yi, Bloodgood , Brenda L, Robichaux , Michael, Malik , Athar N, Ataman , Bulent, Rubin , Alex A, Zieg , Janine, Zhang , Chao, Shokat , Kevan M, Sharma , Nikhil, Cowan , Christop, Greenberg , Michael A chemical genetic approach reveals distinct EphB signaling mechanisms during brain development. Nat Neurosci, 2012-11-11;15(12):1645-54. 2012-11-11 [PMID: 23143520] (Bioassay, Mouse)
• Yu L, Zhou X, Yu J, Huang H, Jiang L, Zhang F, Cao J, Yan M PI3K contributed to modulation of spinal nociceptive information related to ephrinBs/EphBs. PLoS ONE, 2012-08-03;7(8):e40930. 2012-08-03 [PMID: 22879882] (In Vivo, Mouse)
• Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition. Eur. J. Immunol., 2012-05-23;42(6):1562-72. 2012-05-23 [PMID: 22622783] (Bioassay, Mouse)
• EphB regulates L1 phosphorylation during retinocollicular mapping. Mol. Cell. Neurosci., 2012-05-08;50(2):201-10. 2012-05-08 [PMID: 22579729] (Bioassay, Human)
• Maddigan A, Truitt L, Arsenault R EphB receptors trigger Akt activation and suppress Fas receptor-induced apoptosis in malignant T lymphocytes. J. Immunol., 2011-10-28;187(11):5983-94. 2011-10-28 [PMID: 22039307] (Bioassay, Human)
• Pittman AJ, Gaynes JA, Chien CB nev (cyfip2) is required for retinal lamination and axon guidance in the zebrafish retinotectal system. Dev. Biol., 2010-06-09;344(2):784-94. 2010-06-09 [PMID: 20537992] (IHC, Zebrafish)
• Afshari FT, Kwok JC, Fawcett JW Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling. J. Neurosci., 2010-03-24;30(12):4246-55. 2010-03-24 [PMID: 20335460] (Bioassay, Rat)
• Mellott DO, Burke RD Divergent roles for Eph and ephrin in avian cranial neural crest. BMC Dev. Biol., 2008-05-21;8(0):56. 2008-05-21 [PMID: 18495033] (IHC, Chicken)
• Liu Z, Conroy WG, Stawicki TM, Nai Q, Neff RA, Berg DK EphB receptors co-distribute with a nicotinic receptor subtype and regulate nicotinic downstream signaling in neurons. Mol. Cell. Neurosci., 2008-03-18;38(2):236-44. 2008-03-18 [PMID: 18403216] (Bioassay, Chicken)
• Lim BK, Matsuda N, Poo MM Ephrin-B reverse signaling promotes structural and functional synaptic maturation in vivo. Nat. Neurosci., 2008-01-13;11(2):160-9. 2008-01-13 [PMID: 18193042] (In Vivo, Xenopus)
• Nestor MW, Mok LP, Tulapurkar ME, Thompson SM Plasticity of neuron-glial interactions mediated by astrocytic EphARs. J. Neurosci., 2007-11-21;27(47):12817-28. 2007-11-21 [PMID: 18032653] (Bioassay, Mouse)
• Ricard J, Salinas J, Garcia L, Liebl DJ EphrinB3 regulates cell proliferation and survival in adult neurogenesis. Mol. Cell. Neurosci., 2006-02-17;31(4):713-22. 2006-02-17 [PMID: 16483793] (In Vivo, Mouse)
• Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases. J. Cell. Sci., 2006-02-01;119(0):559-70. 2006-02-01 [PMID: 16443753] (Bioassay, Mouse)
• Nakada M, Niska JA, Tran NL, McDonough WS, Berens ME EphB2/R-Ras signaling regulates glioma cell adhesion, growth, and invasion. Am. J. Pathol., 2005-08-01;167(2):565-76. 2005-08-01 [PMID: 16049340] (Bioassay, Human)
• Wang Y, Ying GX, Liu X, Wang WY, Dong JH, Ni ZM, Zhou CF Induction of ephrin-B1 and EphB receptors during denervation-induced plasticity in the adult mouse hippocampus. Eur. J. Neurosci., 2005-05-01;21(9):2336-46. 2005-05-01 [PMID: 15932593] (IHC-Fr, Mouse)
• Nakada M, Niska JA, Miyamori H, McDonough WS, Wu J, Sato H, Berens ME The phosphorylation of EphB2 receptor regulates migration and invasion of human glioma cells. Cancer Res., 2004-05-01;64(9):3179-85. 2004-05-01 [PMID: 15126357] (Bioassay, Human)
• Davy A, Aubin J, Soriano P Ephrin-B1 forward and reverse signaling are required during mouse development. Genes Dev., 2004-03-01;18(5):572-83. 2004-03-01 [PMID: 15037550] (IHC, Mouse)
• Sharfe N, Freywald A, Toro A, Dadi H, Roifman C Ephrin stimulation modulates T cell chemotaxis. Eur. J. Immunol., 2002-12-01;32(12):3745-55. 2002-12-01 [PMID: 12516569] (Bioassay, Human)
• Murai KK, Nguyen LN, Irie F, Yamaguchi Y, Pasquale EB Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling. Nat. Neurosci., 2003-02-01;6(2):153-60. 2003-02-01 [PMID: 12496762] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Efnb1
• Uniprot:
  • AAA53231()