Recombinant Mouse Ephrin-B1 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Mouse Ephrin-B1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse EphB3 Fc Chimera at 2 µg/mL (100 µL/well) can bind Recombinant Mouse Ephrin-B1 Fc Chimera with a linear range of 0.01-0.5 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Ephrin-B1 protein
Mouse Ephrin-B1
(Lys30-Ser229)
Accession # AAA53231
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Lys30
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Efnb1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
49.2 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60 kDa, reducing conditions
Publications
Read Publications using
473-EB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Ephrin-B1 Fc Chimera Protein, CF

  • Cek5-L
  • EFL3
  • EFL-3
  • EFNB1
  • ELK ligand
  • ELK-L
  • EphrinB1
  • Ephrin-B1
  • LERK-2
  • STRA-1

Background

Ephrin-B1, also known as Elk Ligand, LERK2, and Eplg2, is an approximately 45 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains (ECD) of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B1 preferentially interacts with receptors in the EphB family. The binding of Ephrin-B1 to EphB proteins also triggers reverse signaling through Ephrin-B1 (1, 2). Mature mouse Ephrin-B1 consists of a 212 amino acid (aa) ECD, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain (3, 4). Within the ECD, mouse Ephrin-B1 shares 94% and 98% aa sequence identity with human and rat Ephrin-B1, respectively. Ligation by EphB2 enhances shedding of a 35 kDa fragment of the Ephrin-B1 ECD (5). The residual membrane-bound portion is then cleaved by gamma-secretase to release the intracellular domain (6). Ephrin-B1 also associates in cis with Claudin-1, -4, and -5 (7, 8). It is expressed on glomerular podocyte slit diaphragms, developing thymocytes, peripheral T cells, monocytes, macrophages, vascular endothelial cells, cardiomyocytes, osteoclasts, and luteinizing granulosa cells in the ovary (8-13). In the developing nervous system, Ephrin-B1 plays a role in cellular migration, axon guidance, and presynaptic development (14-16). It also regulates developing thymocyte survival, monocyte migration, osteoclast differentiation and function, cardiac muscle morphogenesis, and tumorigenesis (5, 8, 10-12). Ephrin-B1 is up-regulated on reactive astrocytes and on macrophages and T cells found in atherosclerotic plaques (11, 17).
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Shao, H. et al. (1994) J. Biol. Chem. 269:26606.
  4. Fletcher, F.A. et al. (1994) Genomics 24:127.
  5. Tanaka, M. et al. (2007) J. Cell Sci. 120:2179.
  6. Tomita, T. et al. (2006) Mol. Neurodegen. 1:2.
  7. Tanaka, M. et al. (2005) EMBO J. 24:3700.
  8. Genet, G. et al. (2012) Circ. Res. 110:688.
  9. Hashimoto, T. et al. (2007) Kidney Int. 72:954.
  10. Yu, G. et al. (2006) J. Biol. Chem. 281:10222.
  11. Sakamoto, A. et al. (2008) Clin. Sci. 114:643.
  12. Cheng, S. et al. (2012) PLoS ONE 7:e32887.
  13. Egawa, M. et al. (2003) J. Clin. Endocrinol. Metab. 88:4384.
  14. Davy, A. et al. (2004) Genes Dev. 18:572.
  15. Bush, J.O. and P. Soriano (2009) Genes Dev. 23:1586.
  16. McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. USA 106:20487.
  17. Wang, Y. et al. (2005) Eur. J. Neurosci. 21:2336.

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Publications for Ephrin-B1 (473-EB)(27)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Chicken, Virus - Henipavirus, Xenopus, Zebrafish.

We have publications tested in 7 applications: Bioassay, Control, Coprecipitation Assay, Enzyme Assay, IHC, IHC-Fr, In Vivo.


Filter By Application
Bioassay
(17)
Control
(1)
Coprecipitation Assay
(1)
Enzyme Assay
(1)
IHC
(3)
IHC-Fr
(1)
In Vivo
(3)
All Applications
Filter By Species
Human
(7)
Mouse
(14)
Rat
(1)
Chicken
(2)
Virus - Henipavirus
(1)
Xenopus
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using 473-EB Applications Species
Larrañaga, E;Marín-Riera, M;Abad?Lázaro, A;Bartolomé-Català, D;Otero-Tarrazón, A;Fernández?Majada, V;Batlle, E;Sharpe, J;Ojosnegros, S;Comelles, J;Martínez, E; Long-range organization of intestinal 2D-crypts using exogenous Wnt3a micropatterning Nature communications 2025-01-03 [PMID: 39753580] (Bioassay, Mouse) Bioassay Mouse
Chang, C;Banerjee, SL;Park, SS;Zhang, X;Cotnoir-White, D;Desbois, M;Grill, B;Kania, A; Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function bioRxiv : the preprint server for biology 2023-08-29 [PMID: 37693478] (Bioassay, Human, Mouse) Bioassay Human, Mouse
S Kohara, K Ogawa Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells Biomedicines, 2022-12-12;10(12):. 2022-12-12 [PMID: 36551990] (Bioassay, Mouse) Bioassay Mouse
ED Laing, CK Navaratnar, S Cheliout D, SR Petzing, Y Xu, SL Sterling, GA Marsh, LF Wang, M Amaya, DB Nikolov, R Cattaneo, CC Broder, K Xu Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus Proc. Natl. Acad. Sci. U.S.A., 2019-09-23;0(0):. 2019-09-23 [PMID: 31548390] (Coprecipitation Assay, Virus - Henipavirus) Coprecipitation Assay Virus - Henipavirus
MA Fawal, T Jungas, A Kischel, C Audouard, JS Iacovoni, A Davy Cross Talk between One-Carbon Metabolism, Eph Signaling, and Histone Methylation Promotes Neural Stem Cell Differentiation Cell Rep, 2018-06-05;23(10):2864-2873.e7. 2018-06-05 [PMID: 29874574] (Bioassay, Human) Bioassay Human
AK O'Neill, AA Kindberg, TK Niethamer, AR Larson, HH Ho, ME Greenberg, JO Bush Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation J. Cell Biol., 2016-10-17;215(2):217-229. 2016-10-17 [PMID: 27810913] (Control) Control
Mimche P, Brady L, Keeton S, Fenne D, King T, Quicke K, Hudson L, Lamb T Expression of the Receptor Tyrosine Kinase EphB2 on Dendritic Cells Is Modulated by Toll-Like Receptor Ligation but Is Not Required for T Cell Activation. PLoS ONE, 2015-09-25;10(9):e0138835. 2015-09-25 [PMID: 26407069] (Bioassay, Mouse) Bioassay Mouse
Zhang , Gu, Brady , John, Liang , Wei-Chin, Wu , Yan, Henkemeyer , Mark, Yan , Minhong EphB4 forward signalling regulates lymphatic valve development. Nat Commun, 2015-04-13;6(0):6625. 2015-04-13 [PMID: 25865237] (Enzyme Assay, Mouse) Enzyme Assay Mouse
Soskis , Michael, Ho , Hsin-Yi, Bloodgood , Brenda L, Robichaux , Michael, Malik , Athar N, Ataman , Bulent, Rubin , Alex A, Zieg , Janine, Zhang , Chao, Shokat , Kevan M, Sharma , Nikhil, Cowan , Christop, Greenberg , Michael A chemical genetic approach reveals distinct EphB signaling mechanisms during brain development. Nat Neurosci, 2012-11-11;15(12):1645-54. 2012-11-11 [PMID: 23143520] (Bioassay, Mouse) Bioassay Mouse
Yu L, Zhou X, Yu J, Huang H, Jiang L, Zhang F, Cao J, Yan M PI3K contributed to modulation of spinal nociceptive information related to ephrinBs/EphBs. PLoS ONE, 2012-08-03;7(8):e40930. 2012-08-03 [PMID: 22879882] (In Vivo, Mouse) In Vivo Mouse
Show All 27 Publications.

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Bioinformatics

Gene Symbol Efnb1
Uniprot