Recombinant Mouse EphA4 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Mouse EphA4 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse EphA4 Fc Chimera at 2 µg/mL (100 µL/well) can bind recombinant human Ephrin-A5 Fc Chimera with a linear range of 0.16-10 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse EphA4 protein
Mouse EphA4
(Val20-Thr547)
Accession # Q03137
IEGRMD Human IgG1
(Pro100-Lys330)
6 His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Val20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Epha4
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
85.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
110 kDa, reducing conditions
Publications
Read Publications using
641-A4 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MES, NaCl, CHAPS, Imidazole and PEG 3350.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 400 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse EphA4 Fc Chimera Protein, CF

  • Cek8
  • EC 2.7.10
  • EC 2.7.10.1
  • EK8
  • EPH receptor A4
  • EphA4
  • EPH-like kinase 8
  • ephrin type-A receptor 4
  • Hek8
  • receptor protein-tyrosine kinase HEK8
  • SEK
  • Sek1
  • TYRO1 protein tyrosine kinase
  • Tyro1
  • Tyrosine-protein kinase receptor SEK
  • Tyrosine-protein kinase TYRO1

Background

EphA4, also known as Hek8, Tyro1, and Sek, is a 120‑130 kDa glycosylated member of the Eph family of transmembrane receptor tyrosine kinases (1, 2). The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. EphA4 is unusual in its ability to be activated by both Ephrin-A and -B molecules, although its interactions with Ephrin-B2 and -B3 are weaker than with Ephrin-A ligands (3, 4). Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. The 528 amino acid (aa) extracellular domain (ECD) of mouse EphA4 contains an N-terminal Ephrin binding region, a cysteine-rich region, and two fibronectin type III domains (FnIII). The 417 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (5). Within the ECD, mouse EphA4 shares 98.3% and 99.6% aa sequence identity with human and rat EphA4, respectively. Alternate splicing of mouse EphA4 generates a short isoform with a 50 aa deletion within the kinase domain. EphA4 is activated by interactions with Ephrin ligands, triggering a repulsive effect on neurite migration (6, 7). This function is important for the accurate guidance and pathfinding of neurites and axons in the spiral ganglion of the cochlea, the anterior commissure, and the corticospinal tract (6‑8). Neuronal EphA4 interactions with astrocyte‑expressed Ephrins also plays a critical role in long term potentiation by regulating hippocampal neuron dendrite arborization, spine maturation, and function (9‑11). The up‑regulation of EphA4 in gastric carcinoma is negatively correlated with patient survival (12). In glioma, EphA4 associates with FGF R1, and this enhances FGF basic-induced tumor cell migration (13). EphA4 is also involved in morphogenesis of the thymic epithelium and T cell development (14).
  1. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  2. Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
  3. Gale, N.W. et al. (1996) Neuron 17:9.
  4. Qin, H. et al. (2010) J. Biol. Chem. 285:644.
  5. Gilardi-Hebenstreit, P. et al. (1992) Oncogene 7:2499.
  6. Brors, D. et al. (2003) J. Comp. Neurol. 462:90.
  7. Ho, S.K.Y. et al. (2009) Neuroscience 160:784.
  8. Canty, A.J. et al. (2006) Proc. Natl. Acad. Sci. 103:15629.
  9. Murai, K.K. et al. (2003) Nat. Neurosci. 6:153.
  10. Filosa, A. et al. (2009) Nat. Neurosci. 12:1285.
  11. Fu, A.K.Y. et al. (2011) Nat. Neurosci. 14:181.
  12. Oki, M. et al. (2008) World J. Gastroenterol. 14:5650.
  13. Fukai, J. et al. (2008) Mol. Cancer Ther. 7:2768.
  14. Munoz, J.J. et al. (2006) J. Immunol. 177:804.

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Publications for EphA4 (641-A4)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Bioassay, ELISA (Capture), ICC.


Filter By Application
Bioassay
(7)
ELISA (Capture)
(1)
ICC
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(6)
Rat
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using 641-A4 Applications Species
S Gu, WY Fu, AKY Fu, EPS Tong, FCF Ip, X Huang, NY Ip Identification of new EphA4 inhibitors by virtual screening of FDA-approved drugs Sci Rep, 2018;8(1):7377. 2018 [PMID: 29743517] (ELISA (Capture)) ELISA (Capture)
L Schoonaert, L Rué, B Roucourt, M Timmers, S Little, L Chávez-Gut, M Dewilde, P Joyce, A Curnock, P Weber, J Haustraete, G Hassanzade, B De Stroope, L Van Den Bo, P Van Damme, R Lemmens, W Robberecht Identification and characterization of Nanobodies targeting the EphA4 receptor J. Biol. Chem., 2017;292(27):11452-11465. 2017 [PMID: 28526745] (Bioassay, Human) Bioassay Human
Weiss A, Airik R, Bohnenpoll T, Greulich F, Foik A, Trowe M, Rudat C, Costantini F, Adams R, Kispert A Nephric duct insertion requires EphA4/EphA7 signaling from the pericloacal mesenchyme. Development, 2014;141(17):3420-30. 2014 [PMID: 25139858] (Bioassay, Mouse) Bioassay Mouse
Stojanovic A, Fiegler N, Brunner-Weinzierl M, Cerwenka A CTLA-4 is expressed by activated mouse NK cells and inhibits NK Cell IFN-gamma production in response to mature dendritic cells. J Immunol, 2014;192(9):4184-91. 2014 [PMID: 24688023] (Bioassay, Mouse) Bioassay Mouse
Han, Xiaofeng, Xu, Yan, Yang, Yilei, Xi, Jingle, Tian, Wang, Duggineni, Srinivas, Huang, Ziwei, An, Jing Discovery and characterization of a novel cyclic peptide that effectively inhibits ephrin binding to the EphA4 receptor and displays anti-angiogenesis activity. PLoS ONE, 2013;8(11):e80183. 2013 [PMID: 24265799]
Stiffel V, Amoui M, Sheng M, Mohan S, Lau K EphA4 receptor is a novel negative regulator of osteoclast activity. J Bone Miner Res, 2014;29(4):804-19. 2014 [PMID: 23983218] (Bioassay, Mouse) Bioassay Mouse
Afshari FT, Kwok JC, Fawcett JW Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling. J. Neurosci., 2010;30(12):4246-55. 2010 [PMID: 20335460] (Bioassay, Rat) Bioassay Rat
Petros TJ, Williams SE, Mason CA Temporal regulation of EphA4 in astroglia during murine retinal and optic nerve development. Mol. Cell. Neurosci., 2006;32(1):49-66. 2006 [PMID: 16574431] (ICC, Mouse) ICC Mouse
Murai KK, Nguyen LN, Koolpe M, McLennan R, Krull CE, Pasquale EB Targeting the EphA4 receptor in the nervous system with biologically active peptides. Mol. Cell. Neurosci., 2003;24(4):1000-11. 2003 [PMID: 14697664] (Bioassay, Mouse) Bioassay Mouse
Murai KK, Nguyen LN, Irie F, Yamaguchi Y, Pasquale EB Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling. Nat. Neurosci., 2003;6(2):153-60. 2003 [PMID: 12496762] (Bioassay, Mouse) Bioassay Mouse

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Bioinformatics

Gene Symbol Epha4
Uniprot