Recombinant Mouse Cripto Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity

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Recombinant Mouse Cripto Protein Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rmActivin RIB/Fc Chimera at 1 µg/mL (100 µL/well) can bind rmCripto with a linear range of 0.8-100 ng/mL. This protein will also bind rmNodal in a functional ELISA.
Source
Mouse myeloma cell line, NS0-derived mouse Cripto protein
 
Mouse Cripto
(Arg26-Gln150)
Accession # P51865
DI 6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Arg26
Protein/Peptide Type
Recombinant Proteins
Gene
Tdgf1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
20-25 kDa, reducing conditions
Publications
Read Publications using
1538-CR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Cripto Protein

  • CFC-2
  • CR
  • Cripto
  • Cripto-1 growth factor
  • Cripto-1
  • CRIPTOCRGF
  • Epidermal growth factor-like cripto protein CR1
  • TDGF1
  • TDGF-1
  • teratocarcinoma-derived growth factor 1

Background

Cripto is the founding member of the epidermal growth factor-CriptoFRL1Cryptic (EGF-CFC) family of signaling proteins that function in cancer and various developmental processes. These developmental processes include: formation of the germ layers and dorsal organizer, specification of anterior-posterior and left-right axes, and differentiation of heart muscle (1, 2). Other members of the EGF-CFC family include Cryptic, Xenopus FRL-1 and zebrafish OEP (one-eyed pinhead). Overall sequence identity between members of the family is low, but they do share several common domains: a variant EGF-like motif, a novel conserved cysteine-rich domain (called CFC domain), and a C-terminal hydrophobic region. Most EGF-CFC members have a glycosyl-phosphatidylinositol (GPI) anchoring site at the C-terminus and exist as extracellular membrane-anchored proteins. However, naturally-occurring soluble isoforms also exist. Mouse Cripto shares 66% and 34% amino acid identity with human Cripto and zebrafish OEP, respectively (2). Despite weak conservation in amino acid identity, EGF-CFC family members appear to function similarly in assays for phenotypic rescue of zebrafish oep mutants (2). Both secreted and membrane bound forms of Cripto demonstrate biological activity (3).

Cripto, also known as CFC-2 or TDGF-1 (teratocarcinoma-derived growth factor), was originally isolated from an undifferentiated human teratocarcinoma cell line as a potential oncogene. It is overexpressed in many types of cancers and acts as a growth factor for tumors (4). Genetic evidence from mice and zebrafish points to a role for Cripto as an essential cofactor in Nodal signaling. Cripto and OEP mutants display defects in mesoderm induction and heart morphogenesis, similar to phenotypes seen in Nodal mutants (2).

Cripto acts as a cofactor for Nodal by recruiting the Activin type I Receptor, ALK-4, leading to an Act RIIB-ALK4-Cripto-Nodal complex for signaling (1, 3). Cripto also forms a complex with activin and Act RIIs to block activin signaling (5). Work has shown that other TGF-beta superfamily members such as Vg1 and GDF-1 also require EGF-CFC cofactors (6). Cripto can also activate mitogen-activated protein kinase (MAPK) and Akt pathways independently of Nodal by directly binding to a membrane-associated heparan sulfate proteoglycan, glypican-1 (7).

  1. Rosa, F.M. (2002) Science’s STKE http://stke.sciencemag.org/.
  2. Shen, M. and A. Schier (2000) Trends Genet. 16:303.
  3. Yan, Y-T. et al. (2002) Mol. Cell Biol. 22:4439.
  4. Salomon, D. et al. (2000) Endocrine-Rel. Cancer 7:199.
  5. Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5193.
  6. Cheng, S. et al. (2003) Genes & Dev. 17:31.
  7. Bianco, C. et al. (2003) Cancer Research 63:1192.

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Publications for CRIPTO (1538-CR)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Bioassay, In Vivo, cell culture.


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Bioassay
(3)
In Vivo
(1)
cell culture
(1)
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Mouse
(5)
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Bioinformatics

Gene Symbol Tdgf1
Entrez
Uniprot