Recombinant Mouse Common gamma Chain Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Common gamma Chain Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the IL-2-dependent proliferation of MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is 2-10 µg/mL in the presence of 30 µg/mL of soluble IL-2 R beta and 10 ng/mL of recombinant human IL-2.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Common gamma Chain/IL-2 R gamma protein
  

  Mouse Common gamma Chain (Ser25-Ala263) Accession # Q3UPA9	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q3UPA9
• N-terminal Sequence: Ser25
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Il2rg
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 54.5 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Common gamma Chain Fc Chimera Protein, CF

• CD132 antigen
• CD132
• CIDX
• combined immunodeficiency, X-linked
• common cytokine receptor gamma chain
• Common gamma Chain
• common gamma-chain
• cytokine receptor common subunit gamma
• gamma(c)
• IL-2 R gamma
• IL-2 receptor subunit gamma
• IL2R gamma
• IL-2R subunit gamma
• IL2RG
• IL-2RG
• IMD4
• interleukin 2 receptor, gamma
• Interleukin-2 receptor subunit gamma
• P64
• SCIDX
• SCIDX1
• severe combined immunodeficiency

Background

The gamma chain of the high affinity functional mouse IL-2 receptor complex belongs to the hematopoietin receptor family. Mouse Common gamma Chain is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 241 residue extracellular domain, a 21 residue transmembrane domain and an 85 residue cytoplasmic domain. Although Common gamma Chain by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 R gamma  is also designated the common gamma chain ( gamma _c) (1, 2). The site of molecular defects in human X-linked SCID (severe combined immunodeficiency) has been mapped to the human common gamma gene.

1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
2. Noguchi, M. et al. (1993) Science 262:1877.

Publications for Common gamma Chain/IL-2 R gamma (784-MR)(1)

Publication using 784-MR

• Chun, JH;Lim, BS;Roy, S;Walsh, MJ;Abhiraman, GC;Zhangxu, K;Atajanova, T;Revach, OY;Clark, EC;Li, P;Palin, CA;Khanna, A;Tower, S;Kureshi, R;Hoffman, MT;Sharova, T;Lawless, A;Cohen, S;Boland, GM;Nguyen, T;Peprah, F;Tello, JG;Liu, SY;Kim, CJ;Shin, H;Quijano-Rubio, A;Jude, KM;Gerben, S;Murray, A;Heine, P;DeWitt, M;Ulge, UY;Carter, L;King, NP;Silva, DA;Kueh, HY;Kalia, V;Sarkar, S;Jenkins, RW;Garcia, KC;Leonard, WJ;Dougan, M;Dougan, SK;Baker, D; Potent antitumor activity of a designed interleukin-21 mimic bioRxiv : the preprint server for biology 2024-12-07 [PMID: 39677740]

Bioinformatics

• Gene Symbol: Il2rg
• Uniprot:
  • Q3UPA9()