Recombinant Mouse CLEC-2/CLEC1B Protein, CF

When Recombinant Mouse Podoplanin Fc Chimera (Catalog #3244-PL) is coated at 1.0 µg/mL, Recombinant MouseCLEC-2/CLEC1B (Catalog #9117-CL) binds with a typical ED50 of0.1-0.5 μg/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CLEC-2/CLEC1B Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Podoplanin Fc Chimera (Catalog # 3244-PL) is coated at 1.0 µg/mL (100 μL/well), Recombinant Mouse CLEC‑2/CLEC1B binds with a typical ED₅₀ of 0.1-0.5 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse CLEC-2/CLEC1B protein
  Met53-Leu229, with an N-terminal 6-His tag
• Accession #: Q9JL99
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Gene: Clec1b
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 24-39 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CLEC-2/CLEC1B Protein, CF

• 1810061I13Rik
• CLEC1B
• CLEC2
• CLEC-2
• CLEC2B
• CLEC2PRO1384
• C-type lectin domain family 1 member B
• C-type lectin domain family 1, member B
• C-type lectin-like receptor 2
• C-type lectin-like receptor-2
• QDED721

Background

C-type lectin-like receptor 2, also known as CLEC-1B, is a 32 kDa type II transmembrane glycoprotein that is expressed on platelets, megakaryocyte, and liver sinusoidal cells (1-4). Mature mouse CLEC-2 consists of a 31 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) with one C-type lectin domain. Within the ECD, mouse CLEC-2 shares 59% and 90% aa sequence identity with human and rat CLEC-2, respectively. Alternative splicing generates a short isoform that lacks the transmembrane segment. CLEC-2 binding to Podoplanin trigger platelet activation, shedding of GPVI, and cleavage of Fcg RIIA/CD32a (5-8). CLEC-2 expression on platelets is required for the development of lymphatic vessels and valves as well as the maintenance of lymph node high endothelial veinules (HEV) during lymphocyte crossing (7-10). CLEC-2 additionally binds to fucoidan, HIV-1, and the platelet-aggregating snake venom protein Rhodocytin/Aggretin (2, 3, 11). In the liver, soluble CLEC-2 promotes M2 macrophage differentiation and limits inflammatory cytokine production (12).

1. Lee, R.H. and W. Bergmeier (2016) J. Thromb. Haemost. Epub PMID 26749528.
2. Suzuki-Inoue, K. et al. (2006) Blood 107:542.
3. Chaipan, C. et al. (2006) J. Virol. 80:8951.
4. Gitz, E. et al. (2014) Blood 124:2262.
5. Suzuki-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
6. Christou, C.M. et al. (2008) Biochem. J. 411:133.
7. Herzog, B.H. et al. (2013) Nature 502:105.
8. Sweet, D.T. et al. (2015) J. Clin. Invest. 125:2995.
9. Hess, P.R. et al. (2014) J. Clin. Invest. 124:273.
10. Osada, M. et al. (2012) J. Biol. Chem. 287:22241.
11. Manne, B.K. et al. (2013) J. Biol. Chem. 288:7717.
12. Wu, X. et al. (2015) EbioMedicine 2:214.

Bioinformatics

• Gene Symbol: Clec1b
• Uniprot:
  • Q9JL99()