Recombinant Mouse CD99 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse CD99 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to bind Recombinant Mouse PILR‑ alpha (Catalog # 4318-PR) in a functional ELISA. Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
• Source: Mouse myeloma cell line, NS0-derived mouse CD99 protein
  

  Mouse CD99 (Ala27 - Gly137) Accession # AAH19482	IEGRMDP	Mouse IgG2A (Glu98 - Lys330)
  N-terminus		C-terminus

• Accession #: AAH19482
• N-terminal Sequence: Ala27
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cd99
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 37.6 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 50-60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD99 Fc Chimera Protein, CF

• 12E7
• CD99 antigenY homolog
• CD99 molecule
• CD99
• E2 antigen
• HBA71
• MIC2 (monoclonal 12E7)
• MIC2
• MIC2Y
• MSK5X
• pilr-1
• PILR-L
• Protein MIC2
• surface antigen MIC2
• T-cell surface glycoprotein E2

Background

Mouse CD99, also called PILR-L (paired immunoglobulin-like type 2 receptor ligand), is a 25 kDa type I transmembrane glycoprotein. The CD99 family of molecules also includes CD99-L2, Xga, and the pseudogene CD99L1 (1 - 4). The mouse CD99 cDNA encodes a 175 amino acid (aa) precursor with a 28 aa signal sequence, a 109 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 17 aa cytoplasmic region. The ECD contains no identifiable motifs, N-linked glycosylation sites, or cysteine residues, but has acidic, proline- and glycine-rich regions and sites for O-linked glycosylation that are conserved across species (4, 5). Mouse CD99 is thought to be rapidly evolving, and shares only 60% aa identity with rat, plus 40 - 48% aa identity with human, equine and canine CD99 (3, 5). The only known isoform of mouse CD99 appears to have a non-functional signal sequence (5). In contrast, human CD99 has long and short isoforms that show differences in function (6). Mouse CD99 may be either expressed on the surface, or retained within the cell as a homodimer, as found in naive CD4⁺ or CD8⁺ T cells (5, 7). CD99 is expressed by most leukocytes and vascular endothelial cells, especially at contact sites (3, 8). CD99 molecules on one cell can interact with CD99 on adjacent cells. CD99 can also interact with PILR-alpha and PILR-beta , which are expressed on granulocytes, macrophages, NK and dendritic cells (1, 9). The interactions regulate the transendothelial migration of lymphocytes and neutrophils to inflamed tissues (9). Sialylated sugars O-linked to CD99 at Thr45 and Thr50 mediate PILR recognition of CD99. Either is sufficient for inhibitory PILR-alpha recognition, while the Thr45 site is specifically required for activating PILR-beta recognition (10, 11).

1. Wilson, M.D. et al. (2006) Physiol Genomics 27:201.
2. Petri, B. and M.G. Bixel (2006) FEBS J. 273:4399.
3. Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
4. Suh, Y.H. et al. (2003) Gene 307:63.
5. Park, S.H. et al. (2005) Gene 353:177.
6. Byun, H-J. et al. (2006) J. Biol. Chem. 281:34833.
7. Choi, G. et al. (2007) Exp. Mol. Med. 39:746.
8. Bixel, G. et al. (2004) Blood 104:3205.
9. Bixel, G. et al. (2007) Blood 109:5327.
10. Wang, J. et al. (2008) J. Immunol. 180:1686.
11. Tabata, S. et al. (2008) J. Biol. Chem. 283:8893.

Bioinformatics

• Gene Symbol: Cd99
• Uniprot:
  • AAH19482()