Recombinant Mouse CD200 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse CD200 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD200 R1 Fc Chimera (Catalog # 2554-CD) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD200 Fc Chimera that produces 50% of the optimal binding response is appoximately 5‑30 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse CD200 protein
  

  Mouse CD200 (Gln31-Gly 232) Accession #O54901	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: O54901
• N-terminal Sequence: No results obtained: Gln31 predicted
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 49 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 65-75 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD200 Fc Chimera Protein, CF

• antigen identified by monoclonal MRC OX-2
• CD200 antigenMOX1
• CD200 molecule
• CD200
• MOX1
• MOX2
• MOX2MRC
• MRC OX-2 antigen
• MRC
• OX-2 membrane glycoprotein
• OX-2

Background

CD200, also known as OX-2, is a 45 kDa type I transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily (1, 2). The mouse CD200 cDNA encodes a 278 amino acid (aa) precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-likeV-type and one Ig-like C2-type domain (3). Splice variants of CD200 have been described in human but not in mouse. Within the ECD, mouse CD200 shares 76% and 94% aa sequence identity with human and rat CD200, respectively. CD200 is widely but not ubiquitously expressed (4). Its receptor (CD200R) is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, which suggests myeloid cell regulation as the major function of CD200 (5-7). CD200 knockout mice are characterized by increased macrophage number and activation, and are predisposed to autoimmune disorders (8). CD200 and CD200 R associate via their respective N-terminal Ig-like domains (9). In myeloid cells, CD200 R initiates inhibitory signals following receptor-ligand contact (6, 7, 10). In T cells, CD200 functions as a costimulatory molecule that is independent of the CD28 pathway (11). Several additional CD200 R-like molecules have been identified in human and mouse, but their capacity to interact with CD200 is controversial (12, 13). Several viruses encode CD200 homologs which are expressed on infected cells during the lytic phase (14, 15). Like CD200 itself, viral CD200 homologs also suppress myeloid cell activity, enabling increased viral propagation (5, 14-16).

1. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
2. Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
3. Chen, Z. et al. (1997) Biochim. Biophys. Acta 1362:6.
4. Wright, G.J. et al. (2001) Immunology 102:173.
5. Shiratori, I. et al. (2005) J. Immunol. 175:4441.
6. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
7. Fallarino, F. et al. (2004) J. Immunol. 173:3748.
8. Hoek, R.M. et al. (2000) Science 290:1768.
9. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
10. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
11. Borriello, F. et al. (1997) J. Immunol. 158:4548.
12. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
13. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
14. Foster-Cuevas, M. et al. (2004) J. Virol. 78:7667.
15. Cameron, C.M. et al. (2005) J. Virol. 79:6052.
16. Langlais, C.L. et al. (2006) J. Virol. 80:3098.

Publications for CD200/OX2 (3355-CD)(8)

Publications using 3355-CD

• Chen, X;Cui, QQ;Hu, XH;Ye, J;Liu, ZC;Mei, YX;Wang, F;Hu, ZL;Chen, JG; CD200 in dentate gyrus improves depressive-like behaviors of mice through enhancing hippocampal neurogenesis via alleviation of microglia hyperactivation Journal of neuroinflammation 2023-06-30 [PMID: 37391731] (In Vivo, Mouse)
• Chamera, K;Curzytek, K;Kami?ska, K;Trojan, E;Le?kiewicz, M;Tylek, K;Regulska, M;Basta-Kaim, A; Insights into the Potential Impact of Quetiapine on the Microglial Trajectory and Inflammatory Response in Organotypic Cortical Cultures Derived from Rat Offspring Biomedicines 2023-05-09 [PMID: 37239076] (Bioassay, Mouse)
• P Shafiei-Ja, DG Helou, BP Hurrell, E Howard, C Quach, JD Painter, L Galle-Treg, M Li, YE Loh, O Akbari CD200-CD200R immune checkpoint engagement regulates ILC2 effector function and ameliorates lung inflammation in asthma Nature Communications, 2021-05-05;12(1):2526. 2021-05-05 [PMID: 33953190] (In Vivo, Mouse)
• J Casulli, ME Fife, SA Houston, S Rossi, J Dow, ED Williamson, GC Clark, T Hussell, RV D'Elia, MA Travis CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality Nat Commun, 2019-05-09;10(1):2121. 2019-05-09 [PMID: 31073183] (In Vivo, Mouse)
• B Zhu, Y Yu, X Liu, Q Han, Y Kang, L Shi CD200 Modulates S. aureus-Induced Innate Immune Responses Through Suppressing p38 Signaling Int J Mol Sci, 2019-02-03;20(3):. 2019-02-03 [PMID: 30717437] (Bioassay, Mouse)
• Hernangomez M, Klusakova I, Joukal M, Hradilova-Svizenska I, Guaza C, Dubovy P CD200R1 agonist attenuates glial activation, inflammatory reactions, and hypersensitivity immediately after its intrathecal application in a rat neuropathic pain model. J Neuroinflammation, 2016-02-18;13(1):43. 2016-02-18 [PMID: 26891688] (In Vivo, Rat)
• Lyons A, Downer E, Costello D, Murphy N, Lynch M Dok2 mediates the CD200Fc attenuation of Abeta-induced changes in glia. J Neuroinflammation, 2012-05-29;9(0):107. 2012-05-29 [PMID: 22642833] (Bioassay, Mouse)
• Guarda G, Dostert C, Staehli F, Cabalzar K, Castillo R, Tardivel A, Schneider P, Tschopp J T cells dampen innate immune responses through inhibition of NLRP1 and NLRP3 inflammasomes. Nature, 2009-06-03;460(7252):269-73. 2009-06-03 [PMID: 19494813] (Bioassay, Mouse)

Bioinformatics

• Uniprot:
  • O54901(Goat)