Recombinant Mouse CCL9/10/MIP-1 gamma Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CCL9/10/MIP-1 gamma Protein, CF Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED₅₀ for this effect is 0.0900-0.900 µg/mL.
• Source: E. coli-derived mouse CCL9/10/MIP-1 gamma protein
  Gln22-Gln122
• Accession #: Q3U9T8
• N-terminal Sequence: Gln22
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ccl9
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity2
• Theoretical MW: 11.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL9/10/MIP-1 gamma Protein, CF

• C-C motif chemokine 9
• CCF18
• CCL9/10
• chemokine (C-C motif) ligand 9
• macrophage inflammatory protein 1-gamma
• macrophage inflammatory protein-related protein 2
• MIP1 gamma
• MIP-1 gamma
• MRP-2
• SCYA10
• Scya9
• small inducible cytokine A10
• small inducible cytokine A9

Background

Mouse CCL9/10 (also named MIP-1 gamma and MRP-2) is an 11 kDa, secreted, monomeric polypeptide that belongs to the beta (or CC) intercrine family of chemokines (1 - 3). Based on its activity and amino acid (aa) sequence, it is further classified as a member of the NC6 or six cysteine-containing CC subfamily of chemokines (2, 4, 5). This subfamily contains four N-terminally extended chemokines, two human (CCL15 and CCL23) and two mouse (CCL9 and CCL10). Within this subfamily, there are no human-to-rodent interspecies orthologs. Mouse CCL9/10 is synthesized as a 122 aa precursor that contains a 21 aa signal sequence and a 101 aa mature region with six cysteines. As noted, the mature region has an expanded N-terminus relative to other CC family members, and it forms a third intrachain disulfide bond with its two extra cysteines (3 - 7). Mouse CCL9/10 is 75% aa identical to rat CCL9/10 (8). Chemokines are known to undergo proteolytic processing to generate multiple isoforms. NC6 chemokines are usually only marginally active at full length, but are converted to highly active forms upon N-terminal truncation. Mature CCL9, in the presence of inflammatory fluids, is naturally truncated by 28, 29 or 30 aa at the N-terminus, generating a highly active, 8 kDa, 71 - 73 aa CCR1 ligand. In contrast, other CCR1 ligands, CCL3/MIP-1 alpha and CCL5/RANTES, lose their potency when proteolytically processed. CCL9/10 is constitutively secreted, and circulates as a full-length molecule. Any onset of inflammation with subsequent enzyme release may act on local NC6 chemokines, generating early, potent leukocyte chemoattractants (5, 7).

1. Zlotnik, A. and O. Yoshie (2000) Immunity 12:121.
2. Zlotnik, A. et al. (1999) Crit. Rev. Immunol. 19:1
3. Mohamadzadeh, M. et al. (1996) J. Immunol. 156:3102.
4. Haelens, A. et al. (1996) Immunobiology 195:499.
5. Berahovich, R.D. et al. (2005) J. Immunol. 174:7341.
6. Youn, B-S. et al. (1995) J. Immunol. 155:2661.
7. Poltorak, A.N. et al. (1995) J. Inflamm. 45:207.

Publications for CCL9/10/MIP-1 gamma (463-MG/CF)(4)

Publications using 463-MG/CF

• L Li, L Ni, RF Heary, S Elkabes Astroglial TLR9 antagonism promotes chemotaxis and alternative activation of macrophages via modulation of astrocyte-derived signals: implications for spinal cord injury J Neuroinflammation, 2020-02-25;17(1):73. 2020-02-25 [PMID: 32098620] (Cell Culture, Mouse)
• S Sharif, N Ueda, Y Nakatani, LM Wise, S Clifton, Z Lateef, AA Mercer, SB Fleming Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity Front Microbiol, 2019-06-25;10(0):1421. 2019-06-25 [PMID: 31293551] (Binding Assay, Mouse)
• Binder NB, Niederreiter B, Hoffmann O, Stange R, Pap T, Stulnig TM, Mack M, Erben RG, Smolen JS, Redlich K Estrogen-dependent and C-C chemokine receptor-2-dependent pathways determine osteoclast behavior in osteoporosis. Nat. Med., 2009-03-29;15(4):417-24. 2009-03-29 [PMID: 19330010] (Bioassay, Mouse)
• Okamatsu Y, Battaglino R, Spate U, Stashenko P MIP-1 gamma promotes receptor-activator-of-NF-kappa-B-ligand-induced osteoclast formation and survival. J. Immunol., 2004-08-01;173(3):2084-90. 2004-08-01 [PMID: 15265944] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Ccl9
• Uniprot:
  • Q3U9T8()