Recombinant Mouse CCL28 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CCL28 Protein, CF Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR10. The ED₅₀ for this effect is 0.3‑1 µg/mL.
• Source: E. coli-derived mouse CCL28 protein
  Ile23-Arg130
• Accession #: Q9JIL2
• N-terminal Sequence: Ile23
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ccl28
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 12.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL28 Protein, CF

• CCL28
• chemokine (C-C motif) ligand 28
• MEC
• member 28
• MGC71902
• Protein CCK1
• VIC

Background

Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag-1 mouse kidney cDNA library. Mouse CCL28 cDNA encodes a 130 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 108 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10 (GPR2 orphan receptor) which is also the receptor for CCL27/CTACK.

1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.

Publications for CCL28 (533-VI/CF)(8)

Publications using 533-VI/CF

• Y Yan, K Hu, M Fu, X Deng, X Guan, S Luo, M Zhang, Y Liu, Q Hu CCL28 Enhances HSV-2 gB-Specific Th1-Polarized Immune Responses against Lethal Vaginal Challenge in Mice Vaccines, 2022-08-10;10(8):. 2022-08-10 [PMID: 36016177] (Bioassay, Mouse)
• MS Hohmann, DM Habiel, MS Espindola, G Huang, I Jones, R Narayanan, AL Coelho, JM Oldham, I Noth, SF Ma, A Kurkciyan, JL McQualter, G Carraro, B Stripp, P Chen, D Jiang, PW Noble, W Parks, J Woronicz, G Yarranton, LA Murray, CM Hogaboam Antibody-mediated depletion of CCR10+ EphA3+ cells ameliorates fibrosis in IPF JCI Insight, 2021-06-08;0(0):. 2021-06-08 [PMID: 33945505] (Bioassay, Mouse)
• P Wang, X Qi, G Xu, J Liu, J Guo, X Li, X Ma, H Sun CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells Aging (Albany NY), 2019-09-26;11(18):7402-7415. 2019-09-26 [PMID: 31557129] (Neutralization, Mouse)
• K Matsuo, D Nagakubo, S Yamamoto, A Shigeta, S Tomida, M Fujita, T Hirata, I Tsunoda, T Nakayama, O Yoshie CCL28-Deficient Mice Have Reduced IgA Antibody-Secreting Cells and an Altered Microbiota in the Colon J. Immunol., 2017-12-13;0(0):. 2017-12-13 [PMID: 29237777] (Bioassay, Mouse)
• SA Geherin, D Gómez, RA Glabman, G Ruthel, A Hamann, GF Debes IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require ?4?1 Integrin To Migrate between the Peritoneum and Inflamed Skin J. Immunol, 2016-02-05;196(6):2514-25. 2016-02-05 [PMID: 26851219] (Bioassay, Mouse)
• Tregoning , John S, Buffa , Viviana, Oszmiana , Anna, Klein , Katja, Walters , Adam A, Shattock , Robin J A &quot;prime-pull&quot; vaccine strategy has a modest effect on local and systemic antibody responses to HIV gp140 in mice. PLoS ONE, 2013-11-19;8(11):e80559. 2013-11-19 [PMID: 24260419] (In Vivo, Mouse)
• Cha HR, Ko HJ, Kim ED Mucosa-associated epithelial chemokine/CCL28 expression in the uterus attracts CCR10+ IgA plasma cells following mucosal vaccination via estrogen control. J. Immunol., 2011-08-10;187(6):3044-52. 2011-08-10 [PMID: 21832166] (Bioassay, Mouse)
• Hieshima K, Kawasaki Y, Hanamoto H, Nakayama T, Nagakubo D, Kanamaru A, Yoshie O CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells. J. Immunol., 2004-09-15;173(6):3668-75. 2004-09-15 [PMID: 15356112] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Ccl28
• Uniprot:
  • Q9JIL2()