Recombinant Mouse BMPR-IB/ALK-6 Fc Chimera (NS0) Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse BMPR-IB/ALK-6 Fc Chimera (NS0) Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit rhBMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED₅₀ for this effect is 0.5-2 µg/mL in the presence of 30 ng/mL of rhBMP-4.
• Source: Mouse myeloma cell line, NS0-derived mouse BMPR-IB/ALK-6 protein
  

  Mouse BMPR-IB (Leu2 - Lys126) Accession # P36898	DIEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: P36898
• N-terminal Sequence: Leu2
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Bmpr1b
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 40.6 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse BMPR-IB/ALK-6 Fc Chimera (NS0) Protein, CF

• activin receptor-like kinase 6
• ALK-6
• ALK6CDw293
• BMP type-1B receptor
• BMPR1B
• BMPR-1B
• BMPRIB
• BMPR-IB
• bone morphogenetic protein receptor type-1B
• bone morphogenetic protein receptor, type IB
• CDw293 antigen
• CDw293
• EC 2.7.11
• EC 2.7.11.30
• serine/threonine receptor kinase

Background

Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor IB (BMPR-IB), also known as activin receptor-like kinase (ALK)-6, is a one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta  in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IB binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IB is expressed in various tissues during embryogenesis. In adult tissues, BMPR-IB is only found in the brain. The extracellular domain of BMPR-IB shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IB are highly conserved and share 98% sequence identity.

1. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
2. Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.

Publications for BMPR-IB/ALK-6 (444-BNS/CF)(3)

Publications using 444-BNS/CF

• Niessen K, Zhang G, Ridgway JB, Chen H, Yan M ALK1 signaling regulates early postnatal lymphatic vessel development. Blood, 2009-11-10;115(8):1654-61. 2009-11-10 [PMID: 19903896] (ELISA (Standard))
• Tomoeda M, Yamada S, Shirai H, Ozawa Y, Yanagita M, Murakami S PLAP-1/asporin inhibits activation of BMP receptor via its leucine-rich repeat motif. Biochem. Biophys. Res. Commun., 2008-04-11;371(2):191-6. 2008-04-11 [PMID: 18407830] (Immunoprecipitation, Human)
• Close JL, Gumuscu B, Reh TA Retinal neurons regulate proliferation of postnatal progenitors and Muller glia in the rat retina via TGF beta signaling. Development, 2005-07-01;132(13):3015-26. 2005-07-01 [PMID: 15944186] (Bioassay, Rat)

Bioinformatics

• Gene Symbol: Bmpr1b
• Uniprot:
  • P36898()