Recombinant Mouse BMP-8a Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse BMP-8a Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the cell growth of DU145 human prostate carcinoma cells. Miyazaki, H. et al. (2004) Oncogene 23:9326. The ED₅₀ for this effect is 0.5-5 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse BMP-8a protein
  Ala261-His399
• Accession #: P34821
• N-terminal Sequence: Ala261
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Bmp8b
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 15.7 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 19 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HCl.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse BMP-8a Protein, CF

• BMP-8a
• bone morphogenetic protein 8a
• Op2
• Osteogenic Protein 2

Background

BMP‑8, also known as osteogenic protein 2 (OP‑2), was first isolated from a hippocampal library in a screen to identify relatives of BMP‑7/OP‑1 (1). BMPs are a family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF‑ beta ) superfamily. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis. Cellular responses to BMPs are mediated by hetero‑oligomeric complexes of type I and type II serine/threonine kinase receptors (2‑4). BMP‑8a and BMP‑8b, produced from separate genes, share 98% amino acid sequence identity in human but only 74% in mouse within the mature regions. Mouse BMP‑8a is synthesized as a 399 aa precursor protein that is cleaved between Arg260 and Ala261 to release the C‑terminal mature protein. Mature mouse BMP‑8a shares 90% aa sequence identity with mature human BMP‑8a and ‑8b. BMP‑8a is expressed during pregnancy in the deciduum and trophoblast cells, by inner root sheath cells of developing hair follicles, and by the epididymis and spermatids (5‑7). In the mouse it cooperates with BMP‑7 in the maintenance of spermatogenesis but is not required for the initiation of spermatogenesis (7, 8). BMP‑8b, in contrast, is required for both the initiation and maintenance of spermatogenesis (9). BMP‑8a is induced during osteoblast differentiation at the onset of mineralization and during the osteogenic phase of bone repair in osteoblasts and osteocytes (10‑12). BMP‑8a/b is also highly expressed in osteosarcomas (13).

1. Ozkaynak, E. et al. (1992) J. Biol. Chem. 267:25220.
2. Chen, D. et al. (2004) Growth Factors 22:233.
3. Bragdon, B. et al. (2010) Cell Signal. 23:609.
4. Singh, A. and R.J. Morris (2010) Cytokine Growth Factor Rev. 21:299.
5. Zhao, G.-Q. and B.L. Hogan (1996) Mech. Dev. 57:159.
6. Ying, Y. and G.-Q. Zhao (2000) Biol. Reprod. 63:1781.
7. Zhao, G.-Q. et al. (1998) Development 125:1103.
8. Zhao, G.-Q. et al. (2001) Dev. Biol. 240:212.
9. Zhao, G.-Q. et al. (1998) Genes Dev. 10:1657.
10. van der Horst, G. et al. (2002) Bone 31:661.
11. Cho, T.-J. et al. (2002) J. Bone Miner. Res. 17:513.
12. Paic, F. et al. (2009) Bone 45:682.
13. Sulzbacher, I. et al. (2002) J. Clin. Pathol. 55:381.

Publications for BMP-8a (7540-BP)(3)

Publications using 7540-BP

• Verdikt, R;Armstrong, AA;Cheng, J;Hwang, YS;Clark, AT;Yang, X;Allard, P; Metabolic memory of ?9-tetrahydrocannabinol exposure in pluripotent stem cells and primordial germ cells-like cells eLife 2023-12-27 [PMID: 38150302] (Cell Culture, Mouse)
• SKT Ooi, H Jiang, Y Kang, P Allard Examining the Developmental Trajectory of an in Vitro Model of Mouse Primordial Germ Cells following Exposure to Environmentally Relevant Bisphenol A Levels Environmental health perspectives, 2021-09-29;129(9):97013. 2021-09-29 [PMID: 34585602] (Bioassay, Mouse)
• N Li, W Ma, Q Shen, M Zhang, Z Du, C Wu, B Niu, W Liu, J Hua Reconstitution of male germline cell specification from mouse embryonic stem cells using defined factors in vitro Cell Death Differ., 2019-01-25;0(0):. 2019-01-25 [PMID: 30683919] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Bmp8b
• Uniprot:
  • P34821()