Recombinant Mouse BMP-6 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse BMP-6 Protein, CF Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.1‑0.4 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse BMP-6 protein
Ser372-His510
Accession #
N-terminal Sequence
Ser372
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Bmp6
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
15.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17-21 kDa, reducing conditions
Publications
Read Publications using
6325-BM/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HCl.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in 4mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse BMP-6 Protein, CF

  • BMP6
  • BMP-6
  • bone morphogenetic protein 6
  • vegetal related growth factor (TGFB-related)
  • vegetal-related (TGFB related) cytokine
  • VG-1-R
  • VG-1-related protein
  • Vg1-related sequence
  • VGR
  • VGR1
  • Vgr-1

Background

Bone Morphogenetic Protein 6 (BMP‑6), also known as Vgr-1, is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor  beta (TGF-beta ) superfamily. Mouse BMP‑6 is synthesized as a 510 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 18 kDa C‑terminal mature protein. Biologically active BMP‑6 consists of a disulfide-linked homodimer of the mature proteins (1, 2). Mature mouse BMP‑6 shares 96% and 98% aa sequence identity with human and rat BMP‑6, respectively. Cellular responses to BMP‑6 are mediated by hetero-oligomeric complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) serine/threonine kinase receptors (3 - 5). Glycosylation of BMP‑6 is required for its interaction with Activin RIA (6). BMP‑6 induces the expression of Noggin and is subsequently antagonized by Noggin (7, 8). BMP‑6 induces a wide range of cellular responses. It promotes osteoblast differentiation from mesenchymal stem cells (5), chondrocyte maturation (9), Ang II-induced aldosterone production in the adrenal cortex (3), hormone production and responsiveness in ovarian granulosa cells (10), iNOS and TNF-alpha production in macrophages (4), the cell death of B cells (8), and neurite outgrowth (11). BMP‑6 expression is induced in astrocytes surrounding sites of brain injury where it functions as a neuroprotectant (11, 12). BMP‑6 is upregulated during prostate cancer progression and promotes tumor cell metastasis to bone (13). Through interactions with the BMP coreceptor RGM‑C/Hemojuvelin, BMP‑6 plays an important role in iron homeostasis by promoting Hepcidin expression and preventing serum iron overload (14, 15).
  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Lyons, K. et al. (1989) Proc. Natl. Acad. Sci. 86:4554.
  3. Inagaki, K. et al. (2006) Endocrinology 147:2681.
  4. Hong, J.H. et al. (2008) Immunology 128:e442.
  5. Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
  6. Saremba, S. et al. (2008) FEBS J. 275:172.
  7. Haudenschild, D.R. et al. (2004) Cancer Res. 64:8276.
  8. Kersten, C. et al. (2005) BMC Immunol. 6:9.
  9. Grimsrud, C.D. et al. (1999) J. Bone Miner. Res. 14:475.
  10. Shi, J. et al. (2009) Fertil. Steril. 92:1794.
  11. Yabe, T. et al. (2002) J. Neurosci. Res. 68:161.
  12. Zhang, Z. et al. (2006) Neuroscience 138:47.
  13. Dai, J. et al. (2005) Cancer Res. 65:8274.
  14. Meynard, D. et al. (2009) Nat. Genet. 41:478.
  15. Andriopoulos, B. Jr. et al. (2009) Nat. Genet. 41:482.

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Publications for BMP-6 (6325-BM/CF)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Bioassay, Cell Culture.


Filter By Application
Bioassay
(5)
Cell Culture
(1)
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Filter By Species
Human
(1)
Mouse
(5)
All Species
Showing Publications 1 - 6 of 6.
Publications using 6325-BM/CF Applications Species
S Kanazawa, H Okada, H Hojo, S Ohba, J Iwata, M Komura, A Hikita, K Hoshi Mesenchymal stromal cells in the bone marrow niche consist of multi-populations with distinct transcriptional and epigenetic properties Scientific Reports, 2021;11(1):15811. 2021 [PMID: 34349154] (Bioassay, Mouse) Bioassay Mouse
H Omiya, S Yamaguchi, T Watanabe, T Kuniya, Y Harada, D Kawaguchi, Y Gotoh BMP signaling suppresses Gemc1 expression and ependymal differentiation of mouse telencephalic progenitors Scientific Reports, 2021;11(1):613. 2021 [PMID: 33436697] (Bioassay, Mouse) Bioassay Mouse
H Lin, F Shi, S Jiang, Y Wang, J Zou, Y Ying, D Huang, L Luo, X Yan, Z Luo Metformin attenuates trauma-induced heterotopic ossification via inhibition of Bone Morphogenetic Protein signalling J Cell Mol Med, 2020;0(0):. 2020 [PMID: 33169942] (Cell Culture, Mouse) Cell Culture Mouse
YS Lee, YH Kim, YS Jung, KS Kim, DK Kim, SY Na, JM Lee, CH Lee, HS Choi Hepatocyte toll-like receptor 4 mediates lipopolysaccharide-induced hepcidin expression Exp. Mol. Med., 2017;49(12):e408. 2017 [PMID: 29217822] (Bioassay, Human) Bioassay Human
HH Wu, OK Lee Exosomes from mesenchymal stem cells induce the conversion of hepatocytes into progenitor oval cells Stem Cell Res Ther, 2017;8(1):117. 2017 [PMID: 28535778] (Bioassay, Mouse) Bioassay Mouse
Chen , Jiekai, Liu , He, Liu , Jing, Qi , Jing, Wei , Bei, Yang , Jiaqi, Liang , Hanquan, Chen , You, Chen , Jing, Wu , Yaran, Guo , Lin, Zhu , Jieying, Zhao , Xiangjie, Peng , Tianran, Zhang , Yixin, Chen , Shen, Li , Xuejia, Li , Dongwei, Wang , Tao, Pei , Duanqing H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs. Nat Genet, 2013;45(1):34-42. 2013 [PMID: 23202127] (Bioassay, Mouse) Bioassay Mouse

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Bioinformatics

Gene Symbol Bmp6
Uniprot