Recombinant Mouse B7-H3 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse B7-H3 Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance anti-CD3-induced proliferation of mouse CD3⁺ T cells. The ED₅₀ for this effect is typically 1.5-7.5 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse B7-H3 protein
  Val29-Phe244, with a C-terminal 10-His tag
• Accession #: Q8VE98.1
• N-terminal Sequence: Val29
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cd276
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 24.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 38-45 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse B7-H3 Protein, CF

• B7H3
• B7-H3
• B7H34Ig-B7-H3
• B7-H3B7 homolog 3
• CD276 antigen
• CD276 molecule
• CD276
• Costimulatory molecule

Background

T cells require a signal induced by the engagement of the T cell receptor and a “costimulatory” signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Members of the B7 superfamily of counter-receptors were identified by their ability to interact with costimulatory molecules found on the surface of T cells. Members of the B7 superfamily include B7-1 (CD80), B7-2 (CD86), B7-H1 (PD-L1), B7-H2 (B7RP-1), B7-H3, and PD-L2 (1). B7-H3 is expressed at very high levels in immature dendritic cells and at moderate levels on mature dendritic cells, LPS stimulated immature dendritic cells and LPS stimulated monocytes, and at low levels on resting monocytes. B7-H3 binds to activated T cells via an as-of-yet identified receptor. B7-H3 co-stimulates proliferation of T cells and interferon-gamma (IFN-gamma ) production and enhances the induction of cytotoxic T cells. B7-H3 shares 20 - 27% amino acid (aa) identity with other B7 family members (2). Murine B7-H3 is a 259 aa protein containing an extracellular domain, a transmembrane domain and a cytoplasmic domain. Mouse and human B7-H3 share 87% aa identity (3).

1. Coyle, A.J. and J.-C. Gutierrez-Ramos (2001) Nature Immunol. 2:203.
2. Chapoval, A.I. et al. (2001) Nature Immunol. 2:269.
3. Sun, M. et al. (2002) J. Immunol. 168:6294.

Publications for B7-H3/CD276 (1397-B3/CF)(8)

Publications using 1397-B3/CF

• IJG Burvenich, S Parakh, FT Lee, N Guo, Z Liu, HK Gan, A Rigopoulos, GJ O'Keefe, SJ Gong, YW Goh, H Tochon-Dan, FE Scott, M Kotsuma, K Hirotani, G Senaldi, AM Scott Molecular imaging of T cell co-regulator factor B7-H3 with 89Zr-DS-5573a Theranostics, 2018-07-30;8(15):4199-4209. 2018-07-30 [PMID: 30128047] (ELISA (Capture), Mouse)
• X Wei, K Li, G Zhang, Y Huang, J Lv, M Li, L Zhao, C Fan, J Pu, J Hou, H Yuan B7-H3 promoted proliferation of mouse spermatogonial stem cellsthe PI3K signaling pathway Oncotarget, 2017-12-20;9(2):1542-1552. 2017-12-20 [PMID: 29416712] (Bioassay, Mouse)
• X Chen, Y Li, S Blankson, M Liu, D Huang, HP Redmond, J Huang, JH Wang, J Wang B7-H3 Augments Inflammatory Responses and Exacerbates Brain Damage via Amplifying NF-?B p65 and MAPK p38 Activation during Experimental Pneumococcal Meningitis PLoS ONE, 2017-01-31;12(1):e0171146. 2017-01-31 [PMID: 28141831] (In Vivo, Mouse)
• W Gu, X Zhang, Y Yan, Y Wang, L Huang, M Wang, X Shao, Z Chen, W Ji B7-H3 participates in the development of Asthma by augmentation of the inflammatory response independent of TLR2 pathway Sci Rep, 2017-01-17;7(0):40398. 2017-01-17 [PMID: 28094276] (Bioassay, Human)
• B7H3 ameliorates LPS-induced acute lung injury via attenuation of neutrophil migration and infiltration Sci Rep, 2016-08-12;6(0):31284. 2016-08-12 [PMID: 27515382] (In Vivo, Mouse)
• Lutz A, Bachawal S, Drescher C, Pysz M, Willmann J, Gambhir S Ultrasound molecular imaging in a human CD276 expression-modulated murine ovarian cancer model. Clin Cancer Res, 2014-01-03;20(5):1313-22. 2014-01-03 [PMID: 24389327] (Western Blot)
• Chen X, Quinn EM, Ni H, Wang J, Blankson S, Redmond HP, Wang JH, Feng X B7-H3 participates in the development of experimental pneumococcal meningitis by augmentation of the inflammatory response via a TLR2-dependent mechanism. J. Immunol., 2012-06-01;189(1):347-55. 2012-06-01 [PMID: 22661093] (In Vivo, Mouse)
• Zhang G, Wang J, Kelly J B7-h3 augments the inflammatory response and is associated with human sepsis. J. Immunol., 2010-08-09;185(6):3677-84. 2010-08-09 [PMID: 20696859] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Cd276
• Uniprot:
  • Q8VE98.1()