Recombinant Mouse B7-H3 Fc Chimera Protein, CF

Recombinant Mouse B7-H3 Fc Chimera (Catalog # 10946-B3) inhibits Mouse CD3 epsilon Antibody (Clone # 145-2C11, MAB484) induced proliferation of Mouse T cells. The ED50 for this effect is 0.06-0.72 µg/mL.

2 μg/lane of Recombinant Mouse B7‑H3 Fc Chimera Protein (Catalog # 10946-B3) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-73 kDa and 130-150 kDa, respectively.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse B7-H3 Fc Chimera Protein, CF Summary

• Details of Functionality:

  Measured by its ability to inhibit Mouse CD3 epsilon Antibody induced proliferation of Mouse T cells. The ED₅₀ for this effect is 0.06-0.72 µg/mL.

• Source: Mouse myeloma cell line, NS0-derived mouse B7-H3 protein
  

  Mouse B7-H3 (Val29-Phe244) Accession # Q8VE98.1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q8VE98.1
• N-terminal Sequence: Val29
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 65-73 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse B7-H3 Fc Chimera Protein, CF

• B7H3
• B7-H3
• B7H34Ig-B7-H3
• B7-H3B7 homolog 3
• CD276 antigen
• CD276 molecule
• CD276
• Costimulatory molecule

Background

T cells require a signal induced by the engagement of the T cell receptor and a “costimulatory” signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Members of the B7 superfamily of counter-receptors were identified by their ability to interact with costimulatory molecules found on the surface of T cells. Members of the B7 superfamily include B7-1 (CD80), B7-2 (CD86), B7-H1 (PD-L1), B7-H2 (B7RP-1), B7-H3, and PD-L2 (1). B7-H3 is expressed at very high levels in immature dendritic cells at moderate levels on mature dendritic cells, LPS stimulated immature dendritic cells and LPS stimulated monocytes, and at low levels on resting monocytes. B7-H3 binds to activated T cells via an as-of-yet identified receptor. B7-H3 co-stimulates proliferation of T cells and interferon-gamma (IFN-gamma ) production and enhances the induction of cytotoxic T cells. B7-H3 shares 20 - 27% amino acid (aa) identity with other B7 family members (2). Murine B7-H3 is a 259 aa protein containing an extracellular domain, a transmembrane domain and a cytoplasmic domain. Mouse and human B7-H3 share 87% aa identity (3).

1. Coyle, A.J. and J.-C. Gutierrez-Ramos (2001) Nature Immunol. 2:203.
2. Chapoval, A.I. et al. (2001) Nature Immunol. 2:269.
3. Sun, M. et al. (2002) J. Immunol. 168:6294.

Bioinformatics

• Uniprot:
  • Q8VE98.1()