Recombinant Mouse Angiopoietin-like 7 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Angiopoietin-like 7 Protein, CF Summary

• Details of Functionality: Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED₅₀ for this effect is 60-240 ng/mL in the presence of a cross‑linking Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Angiopoietin-like Protein 7/ANGPTL7 protein
  Met1-Pro337, with a C-terminal 10-His tag
• Accession #: NP_001034643
• N-terminal Sequence: No results obtained: Gln22 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: Angptl7
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 37.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 45-55 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in NaPO₄ and NaCl.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Angiopoietin-like 7 Protein, CF

• angiopoietin-like 7
• Angiopoietin-like factor
• Angiopoietin-like Protein 7
• angiopoietin-related protein 7
• ANGPTL7
• AngX
• CDT6
• CDT6angiopoietin-like factor (CDT6)
• Cornea-derived transcript 6 protein
• dJ647M16.1
• RP4-647M16.2

Background

Angiopoietin-like 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted, 45‑50 kDa glycoprotein member of the angiopoietin‑like family of molecules. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain (1‑3). Mature mouse ANGPTL7 is 316 amino acids (aa) in length. It shares 88% and 98% aa sequence identity with human and rat ANGPTL7, respectively. The ANGPTL7 monomer forms homotetramers via its coiled coil domain (4, 5). ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera (3, 5). Its production is up‑regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha (5-7). ANGPTL7 expression is up‑regulated under conditions of increased ocular pressure (IOP). Since IOP is a risk factor for glaucoma, ANGPTL7 may represent a defense mechanism to reduce pressure on the retina. It appears that IOP is a consequence of increased ECM deposition, a major component of which is fibronectin. In this regard, ANGPTL7 is reported to both decrease fibronectin expression and promote MMP1 expression, actions that would tend to reduce organized ECM deposition (8). When overexpressed in tumor cells, it promotes collagen and proteoglycan deposition but inhibits tumor xenograft progression and tumor angiogenesis (4). ANGPTL7, when used in combination with SCF, Thrombopoietin, IGF-II, and FGF acidic, enhances the expansion and engraftment of human and mouse hematopoietic stem cells (9).

1. Oike, Y. et al. (2009) Circ. J. 73:2192.
2. Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70.
3. Peek, R. et al. (1998) Invest. Opthalmol. Vis. Sci. 39:1782.
4. Peek, R. et al. (2002) J. Biol. Chem. 277:686.
5. Kuchtey, J. et al. (2008) Invest. Opthalmol. Vis. Sci. 49:3438.
6. Nehme, A. et al. (2009) BMC Med. Genomics 2:58.
7. Stevens, A.L. et al. (2008) Arthritis Rheum. 58:489.
8. Comes, N. et al. (2011) Genes Cells 16:243.
9. Zhang, C.C. et al. (2006) Nat. Med. 12:240.

Bioinformatics

• Gene Symbol: Angptl7
• Uniprot:
  • NP_001034643()