Recombinant Mouse Activin RIIB Fc Chimera Protein, CF

Recombinant Mouse Activin RIIB Fc Chimera (Catalog # 3725-RB) inhibits Activin A-induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The ED50 for this effect is 5-30 ng/mL in the presence of 3 ng/mL of Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC).

1 μg/lane of Recombinant Mouse Activin RIIB Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 63 kDa and 110 kDa, respectively.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Activin RIIB Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit Activin A-induced hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED₅₀ for this effect is 5-30 ng/mL in the presence of 3 ng/mL of recombinant human Activin A.
• Source: Mouse myeloma cell line, NS0-derived mouse Activin RIIB protein
  

  Mouse Activin RIIB (Ser19-Thr134) Accession # P27040	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: P27040
• N-terminal Sequence: Ser19
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 40.5 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 61-63 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Activin RIIB Fc Chimera Protein, CF

• activin A receptor, type IIB
• Activin receptor type IIB
• activin receptor type-2B
• Activin RIIB
• ActivinRIIB
• ACTRIIB
• ActR-IIB
• ACVR2B
• EC 2.7.11
• EC 2.7.11.30
• MGC116908

Background

Activin molecules and other members of the TGF-beta superfamily exert their biological effects through heteromeric complexes of type I and a type II serine/threonine kinase receptors (1, 2). Ligands bind to the type II receptor (such as ActRIIB) which then associates with a type I receptor to initiate signal transduction (3, 4). The mature mouse ActRIIB consists of a 119 amino acid (aa) extracellular domain, a 21 aa transmembrane segment, and a 378 aa cytoplasmic region that includes the kinase domain and a C-terminal PDZ-binding motif (5). ActRIIB exists in four alternately spliced forms that are distinguished by the deletion of juxtamembrane stretches in the extra and/or intracellular regions (5). Particular activin isoforms bind with different high affinities to the various type II receptor isoforms (5). Within the ECD, mouse ActRIIB shares 99% aa sequence identity with human and rat ActRIIB. It shares 53% aa sequence identity with ActRIIA. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2, and BMP-7 with lower affinities. ActRIIB mediated signaling can be modulated by several accessory proteins. Interactions with the GPI linked RGM-A/DRAGON lower the threshold for BMP-2 and BMP-4 induced signaling (6). ActRIIB forms a ternary complex with activin A and cripto which prevents association with the type I receptor ActRIB, thereby interfering with activin induced signaling (7). ActRIIB can also form a ternary complex with activin A and endoglin (8). The C-terminal tail of ActRIIB specifically binds the PDZ domain of the cytoplasmic protein ARIP2 which enhances receptor internalization and hinders activin induced responses (9). Although three variants of ARIP2 have been identified, only the full length isoform modulates ActRII internalization (10).

1. Welt, C. et al. (2002) Exp. Biol. Med. 227:724.
2. Chen, Y.-C., et al. (2006) Exp. Biol. Med. 231:534.
3. Attisano, L. et al. (1996) Mol. Cell Biol. 16:1066.
4. Allendorph, G.P. et al. (2006) Proc. Natl. Acad. Sci. 103:7643.
5. Attisano, L. et al. (1992) Cell 68:97.
6. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
7. Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. 100:5193.
8. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
9. Matsuzaki, T. et al. (2002) J. Biol. Chem. 277:19008.
10. Liu, Z.H. et al. (2006) J. Endocrinol. 189:409.

Publications for Activin RIIB (3725-RB)(2)

Publications using 3725-RB

• Ohsawa Y, Hagiwara H, Nakatani M, Yasue A, Moriyama K, Murakami T, Tsuchida K, Noji S, Sunada Y Muscular atrophy of caveolin-3-deficient mice is rescued by myostatin inhibition. J. Clin. Invest., 2006-10-12;116(11):2924-34. 2006-10-12 [PMID: 17039257] (In Vivo, Mouse)
• Close JL, Gumuscu B, Reh TA Retinal neurons regulate proliferation of postnatal progenitors and Muller glia in the rat retina via TGF beta signaling. Development, 2005-07-01;132(13):3015-26. 2005-07-01 [PMID: 15944186] (Bioassay, Rat)

Bioinformatics

• Uniprot:
  • P27040()