Recombinant Human VSIG3 Fc Chimera Avi-tag Protein, CF

When Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera Protein (Catalog # 7126-B7) is immobilized at 2.5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human VSIG3 Fc Chimera Avi-tag (Catalog # AVI9229) that produces 50% of the optimal binding response is found to be approximately 3‑15 μg/mL.

2 μg/lane of Biotinylated Recombinant Human VSIG3 Fc Chimera Avi-tag (Catalog # AVI9229) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human VSIG3 Fc Chimera Avi-tag Protein, CF Summary

• Additional Information: Biotinylated
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera Protein (Catalog # 7126-B7) is immobilized at 2.5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human VSIG3 Fc Chimera Avi-tag (Catalog # AVI9229) that produces 50% of the optimal binding response is found to be approximately 3-15 μg/mL. Measured by its ability to inhibit anti-CD3 antibody induced IL-17 or IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 1-10 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human VSIG3 protein
  

  Human VSIG3 (Leu23-Gly245) Accession # Q5DX21.3	IEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
  N-terminus			C-terminus

• Accession #: Q5DX21.3
• N-terminal Sequence: Leu23
• Structure / Form: Disulfide-linked homodimer, biotinylated via Avi-tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW:

  52 kDa

  .
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 62-69 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human VSIG3 Fc Chimera Avi-tag Protein, CF

• Brain and testis-specific immunoglobulin superfamily protein
• BTIGSF
• BT-IgSF
• cancer/testis antigen 119
• CT119
• CXADR like 1
• CXADRL1
• IGSF11
• Igsf13
• immunoglobulin superfamily member 11
• immunoglobulin superfamily, member 11
• MGC35227
• V-set and immunoglobulin domain containing 3
• V-set and immunoglobulin domain-containing protein 3
• VSIG3
• VSIG3brain and testis-specific immunoglobin superfamily protein

Background

VSIG3, also known as IGSF11, BT-IgSF, and CLMP, is an approximately 50 kDa transmembrane adhesion protein (1). Mature human VSIG3 consists of a 219 amino acid (aa) extracellular domain (ECD) that contains two tandem Ig-like domains, a 21 aa transmembrane segment, and a 169 aa cytoplasmic domain (2). Within the ECD, human VSIG3 shares 95% aa sequence identity with mouse and rat VSIG3. Alternative splicing generates additional isoforms with a substituted signal peptide that may also have a deletion in the second Ig-like domain (3). VSIG3 is expressed on epithelial and endothelial cells, neurons and glial cells, and platelets (2-4). It localizes to epithelial tight junctions and mediates homophilic in trans cell adhesion (3-5). VSIG3 also localizes to neuronal postsynaptic densisties where it recruits the GluA1 and GluA2 subunits of AMPA receptors and supports excitatory synaptic transmission (6). The short isoform can be up-regulated in gastric cancer (7). In zebrafish, VSIG3 is expressed in melanophores and their precursors and plays a role in the development and patterning of pigment cells (8).

1. Schreiber, J. et al. (2014) Adv. Neurobiol. 8:21.
2. Suzu, S. et al. (2002) Biochem. Biophys. Res. Commun. 296:1215.
3. Katoh, M. and M. Katoh (2003) Int. J. Oncol. 23:525.
4. Raschperger, E. et al. (2004) J. Biol. Chem. 279:796.
5. Harada, H. et al. (2005) J. Cell. Physiol. 204:919.
6. Jang, S. et al. (2016) Nat. Neurosci. 19:84.
7. Watanabe, T. et al. (2005) Cancer Sci. 96:498.
8. Eom, D.S. et al. (2012) PLoS Genet. 8:e1002899.

Bioinformatics

• Uniprot:
  • Q5DX21.3()