Recombinant Human Vitronectin Protein, Animal-Free

Animal-Free™ Recombinant Human Vitronectin (Catalog # BT-VTN-AFL) supports the adhesion of B16-F1 mouse melanoma cells. The ED50 for this effect is 10.0-120 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Vitronectin Protein, Animal-Free Summary

• Additional Information: N-terminal Variant
• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. The ED₅₀ for this effect is 10.0-120 ng/mL.
• Source: E. coli-derived human Vitronectin protein
  Val62-Glu292 with a C-terminal 6-His tag
  Produced using non-animal reagents in an animal-free laboratory.
• Accession #: P04004.1
• N-terminal Sequence: Met & Val62
• Protein/Peptide Type: Animal-Free Recombinant Proteins
• Purity: >90%, by HPLC
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and TCEP with Trehalose.
• Purity: >90%, by HPLC
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Vitronectin Protein, Animal-Free

• Complement S-protein
• epibolin
• Serum Spreading Factor
• Serum-spreading factor
• Somatomedin B
• S-protein
• V75
• vitronectin (serum spreading factor, somatomedin B, complement S-protein)
• Vitronectin
• VN
• VNT
• VTN

Background

Vitronectin is a large glycoprotein found in blood and the extracellular matrix (ECM). As one of the major cell adhesion molecules in plasma, it interacts with integrins in the extracellular environment. Due to its many functions for cell biology including supporting the growth, maintenance and differentiation of stem cells, Vitronectin is a crucial molecule in stem cell therapy applications.

A truncated, E. coli derived version of human Vitronectin (aa62-292) has been optimized for large scale stem cell culture applications. This Vitronectin contains the RGD sequence necessary for interaction with cell-surface receptors to maintain its ability to act as a stem cell substrate.

1. Lu, X. et al. (2024) Cells 13:1566.

Bioinformatics

• Uniprot:
  • P04004.1()