Recombinant Human URB Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human URB Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit proliferation of HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 1-4 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human URB protein
Met129-Tyr950 & Ser79-Tyr950, both with C-terminal 6-His tag
Accession #
N-terminal Sequence
Met129 & Ser79
Protein/Peptide Type
Recombinant Proteins
Gene
CCDC80
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
100.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
125-150 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human URB Protein, CF

  • CCDC80
  • coiled-coil domain containing 80
  • down-regulated by oncogenes protein 1
  • DRO1
  • SSG1
  • Steroid-sensitive protein 1
  • URB

Background

URB (upregulated in BRS-3 deficient mice) is a 150 kDa, secreted glycoprotein that belongs to the sushi-repeat-containing protein superfamily (1, 2). Sushi repeats, otherwise known as short consensus repeats (SCRs), are 60 amino acid (aa) sequences usually involved in protein-protein interaction. They are characterized by the presence of four Cys, two Pro, one Gly and one Trp (3). Human URB is synthesized as a 950 aa precursor that contains a 21 aa signal sequence and a 929 aa mature region. The mature molecule contains three extended sushi/SCR domains of approximately 140 aa each. They bear resemblance to the fifth sushi-repeat in human SPRX (4). The three lie between aa 141 - 281, 615 - 760, and 771 - 913, respectively. Between the first and second SCR lie two amino acid-rich regions, a Thr-rich domain (aa 347 - 404), and a Lys-rich domain (aa 487 - 588). Three potential N-linked glycosylation sites exist in the last two SCR’s, while six potential bipartite nuclear localization signals (NLS) occur between aa 420 - 780. There are two potential alternate splice forms for human URB. One is 594 aa in length, and shows a simple truncation at Ser594. This effectively removes the second and third SCRs and two bipartite NLS (5). The second is 553 aa in length and shows a simple truncation after Lys553. This eliminates four bipartite NLSs, the second and third SCRs, and part of the Lys-rich domain (6). Full-length human URB is 83%, 84% and 87% aa identical to rat, mouse and bovine URB, respectively. URB is found in chondrocytes and appears to be downregulated upon CFU-Fibroblast differentiation (1). Thus, it may play a role in skeletogenesis.

  1. Liu, Y. et al. (2004) Biochem. Biophys. Res. Commun. 322:497.
  2. Aoki, K. et al. (2002) Biochem. Biophys. Res. Commun. 290:1282.
  3. Anatova, J. et al. (1989) Biochemistry 28:4754.
  4. Meindl, A. et al. (1995) Hum. Mol. Genet. 4:2339.
  5. Isogai, T. et al. (2002) GenBank Accession # BAC11475.
  6. Strausberg, R.L. et al. (2002) GenBank Accession # AAH86876.

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Bioinformatics

Gene Symbol CCDC80
Uniprot