Recombinant Human SUMO1 AMC is a fluorogenic substrate for some SUMO-specific isopeptidases. Release of AMC fluorescence can be monitored with an excitation wavelength of 345 nM and an emission wavelength of 445 nM. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human SUMO1 AMC concentration of 0.1-1 μM.
11 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 °C as supplied.
3 months, -70 °C under sterile conditions after opening.
1.13 mg/ml (100 µM) in 50 mM HEPES pH 7.5, 100 mM NaCl
>95%, by HPLC.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human SUMO1 AMC Protein, CF
GAP modifying protein 1
GAP-modifying protein 1
small ubiquitin-related modifier 1
SMT3 homolog 3
SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
SMT3 suppressor of mif two 3 homolog 1 (yeast)
Ubiquitin-homology domain protein PIC1
ubiquitin-like 1 (sentrin)
Ubiquitin-like protein SMT3C
Ubiquitin-like protein UBL1
Human Small Ubiquitin-like Modifier 1 (SUMO1), also known as Sentrin, UBL1, and SMT3C, is synthesized as a 101 amino acid (aa) propeptide with a predicted molecular weight of 11.5 kDa. Human SUMO1 is the most unique of the four identified SUMO proteins and shares only 44%, 47%, and 41% aa sequence identity with SUMO2, SUMO3, and SUMO4, respectively. In contrast, human SUMO1 shares 100% aa sequence identity with the mouse ortholog. SUMOs are a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed SUMOylation (1-3). All SUMO proteins share a conserved Ubiquitin domain and a C-terminal diglycine cleavage/attachment site. Following cleavage of a four aa C-terminal prosegment, the C-terminal glycine residue of SUMO1 is enzymatically attached to a lysine residue on a target protein. In humans, SUMO1 is conjugated to a variety of molecules in the presence of the SAE1/UBA2 SUMO-activating (E1) enzyme and the UBE2I/Ubc9 SUMO-conjugating (E2) enzyme (4,5). In yeast, the SUMO-activating (E1) enzyme is Aos1/Uba2p (6). SUMOylation can occur without the requirement of a specific SUMO ligase (E3), where SUMO1 is transferred directly from UBE2I/Ubc9 to specific substrates. In Alzheimer's disease models SUMO1 has been shown to influence the generation of Amyloid-beta peptide by promoting the accumulation of BACE-1 (7). Covalent modification of Phosphatase and Tensin Homolog Deleted on Chromosome (PTEN) by SUMO1 is thought to regulate tumorigenesis by retaining PTEN at the plasma membrane, an effect that suppresses PI 3-Kinase/Akt-dependent tumor growth (8).
This fluorogenic substrate for SUMO1 hydrolases is based on the carboxy-terminus derivatization of SUMO1 with 7-amido-4-methylcoumarin (AMC). SUMO1 AMC is useful for studying SUMO1 hydrolases when detection sensitivity or continuous monitoring of activity is essential.
Desterro, J.M. et al. (1997) FEBS. Lett. 417:297.
Bettermann, K. et al. (2012) Cancer Lett. 316:113.
Praefcke, G.J. et al. (2012) Trends Biochem. Sci. 37:23.
Okuma, T. et al. (1999) Biochem. Biophys. Res. Commun. 254:693.
Tatham, M.H. et al. (2001) J. Biol. Chem. 276:35368.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our Recombinant Human SUMO1 AMC Protein, CF and receive a gift card or discount.