Recombinant Human Stabilin-2 His-tag Protein, CF

When Recombinant Human Stabilin-2 His-tag Protein (Catalog # 11086-S2) is immobilized at 2.00 μg/mL (100 μL/well), Biotinylated Hyaluronan binds with an ED50 of 1.50-7.50 ng/mL.

2 μg/lane of Recombinant Human Stabilin‑2 His-tag Protein (Catalog # 11086-S2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 260-300 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Stabilin-2 His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Stabilin-2 His-tag Protein is immobilized at 2.00 μg/mL (100 μL/well), Biotinylated Hyaluronan binds with an ED 50 of 1.50-7.50 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Stabilin-2 protein Ser20-Thr2458, with a C-terminal 6-His tag
• Accession #: Q8WWQ8.3
• N-terminal Sequence: Ser20
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 265 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 260-300 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Stabilin-2 His-tag Protein, CF

• CD44-like precursor FELL
• DKFZP434E0321
• FAS1 EGF-like and X-link domain containing adhesion molecule-2
• FAS1 EGF-like and X-link domain-containing adhesion molecule 2
• fasciclin egf-like, laminin-type egf-like, and link domain-containing scavengerreceptor-2
• Fasciclin, EGF-like, laminin-type EGF-like and link domain-containing scavengerreceptor 2
• FEEL2
• FEEL-2
• FELE-2
• FELL
• FELL-2
• FEX2
• HARE
• HAREFELL2
• hepatic hyaluronan clearance receptor
• Hyaluronan receptor for endocytosis
• hyaluronic acid receptor for endocytosis
• STAB2
• STAB-2
• stabilin 2
• Stabilin2
• Stabilin-2

Background

Stabilin--2, also known as HARE (Hyaluronic Acid Receptor for Endocytosis) and FEEL-2 (Fasciclin, EGF--like, laminin type EGF--like, and Link domain containing scavenger receptor 2), is a type I transmembrane multi-domain protein that is the closest homolog of stabilin-1. It is a scavenger receptor that is expressed on sinusoidal endothelial cells of liver, spleen, and lymph node (1, 2). Its 2439 amino acid (aa) extracellular domain contains seven fasciclin domains, multiple EGF--like and laminin type EGF--like domains, and a link domain related to molecules of the TSG-6 superfamily (3). The 72 aa cytoplasmic tail of Stabilin-2 contains a motif that allows the AP2 classical cargo adaptor to direct cargo into clathrin-coated pits (4). Stabilin-2 also mediates the endocytosis of chondroitin and chondroitin sulfate, advanced glycosylation end-product (AGE), collagen N-terminal propeptides and acetylated LDL (4-7). Stabilin-2 has been found to regulate the cancer progression, and high expression of Stabilin-2 was reported to predict poor tumor prognosis in NSCLC (8).

1. Politz, O. et al. (2002) Biochem. J. 362:155.
2. Harris, E.N. and F. Cabral (2019) Biomolecules 9:273.
3. Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
4. Hansen, B. et al. (2005) Exp. Cell Res. 303:160.
5. Harris, E.N. et al. (2004) J. Biol. Chem. 279:36201.
6. Harris, E.N. et al. (2007) J. Biol. Chem. 282:2785.
7. Tamura, Y. et al. (2003) J. Biol. Chem. 278:12613.
8. Yong, J. et al. (2021) Bioengineered 12:3426.

Bioinformatics

• Uniprot:
  • Q8WWQ8.3()