Recombinant Human Stabilin-2 (aa 2198-2295) Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human Stabilin-2 (aa 2198-2295) Protein, CF Summary

• Details of Functionality: Measured by its ability to bind biotinylated Hyaluronan in a functional ELISA with an apparent K D for this binding is <0.1 nM.
• Source: E. coli-derived human Stabilin-2 protein

  MNHKVHM

  Human Stabilin (Gly2198 - Val2295) Accession # Q8WWQ8

  6-His tag

  N-terminus

  C-terminus

• Accession #: Q8WWQ8
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: STAB2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 12.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Stabilin-2 (aa 2198-2295) Protein, CF

• CD44-like precursor FELL
• DKFZP434E0321
• FAS1 EGF-like and X-link domain containing adhesion molecule-2
• FAS1 EGF-like and X-link domain-containing adhesion molecule 2
• fasciclin egf-like, laminin-type egf-like, and link domain-containing scavengerreceptor-2
• Fasciclin, EGF-like, laminin-type EGF-like and link domain-containing scavengerreceptor 2
• FEEL2
• FEEL-2
• FELE-2
• FELL
• FELL-2
• FEX2
• HARE
• HAREFELL2
• hepatic hyaluronan clearance receptor
• Hyaluronan receptor for endocytosis
• hyaluronic acid receptor for endocytosis
• STAB2
• STAB-2
• stabilin 2
• Stabilin2
• Stabilin-2

Background

Stabilin-2, also known as HARE (hyaluronan receptor for endocytosis) and FEEL-2 (fasciclin, EGF-like, laminin type EGF-like, and link domain containing scavenger receptor 2), is a type I transmembrane multi-domain protein that is the closest homolog of stabilin-1 (1, 2). It is a scavenger receptor that is expressed on sinusoidal endothelial cells of liver, spleen, and lymph node (1, 2). Its 2439 amino acid (aa) extracellular domain contains seven fasciclin domains, multiple EGF-like and laminin type EGF-like domains, and a link domain related to molecules of the TSG-6 superfamily (3). The 72 aa cytoplasmic tail of Stabilin-2 contains a motif that allows the AP2 classical cargo adaptor to direct cargo into clathrin-coated pits (4). As a recycling receptor, stabilin-2 cycles from the membrane to clathrin-coated pits each 10 - 15 minutes, with about two thirds of the protein found within the endocytic system at any one time (4). The link domain binds and mediates the systemic clearance of hyaluronan (HA) (2 - 6). Within the link domain (aa 2198 - 2295), human stabilin-2 shares 86% and 89% aa identity with mouse and rat stabilin-2, respectively. Total human HA is about 15 grams, of which about 5 grams are cleared each day (5). Stabilin-2 also mediates the endocytosis of chondroitin and chondroitin sulfate, advanced glycosylation end-product (AGE), collagen N-terminal propeptides and acetylated LDL (4 - 7). Human stabilin-2 mRNA encodes a 2251 aa, 315 kDa protein that produces an isoform of 190 kDa through proteolytic cleavage (2, 6). The 190 kDa form lacks some N-terminal fasciclin and EGF-like domains, but shows similar activities when compared with the 315 kDa form (2, 6).

1. Politz, O. et al. (2002) Biochem. J. 362:155.
2. Zhou, B. et al. (2003) Glycobiology 13:339.
3. Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
4. Hansen, B. et al. (2005) Exp. Cell Res. 303:160.
5. Harris, E.N. et al. (2004) J. Biol. Chem. 279:36201.
6. Harris, E.N. et al. (2007) J. Biol. Chem. 282:2785.
7. Tamura, Y. et al. (2003) J. Biol. Chem. 278:12613.

Bioinformatics

• Gene Symbol: STAB2
• Uniprot:
  • Q8WWQ8()