Recombinant Human Sphingosine Kinase 2/SPHK2 Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human Sphingosine Kinase 2/SPHK2 Protein, CF Summary

• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Sphingosine Kinase 2/SPHK2 protein
  Asn2-Pro654, with an N-terminal 5-His tag
• Accession #: Q9NRA0
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Enzymes
• Gene: SPHK2
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-85 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in MES, NaCl, Glycerol and DTT.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Sphingosine Kinase 2/SPHK2 Protein, CF

• EC 2.7.1.91
• SK 2
• SK2
• sphingosine kinase 2
• sphingosine kinase type 2 isoform
• SPHK2
• SPK 2
• SPK2

Background

Sphingosine kinases catalyze the phosphorylation of sphingosine to sphingosine-1-phosphate, a lipid messenger molecule involved in the regulation of processes such as growth, differentiation, and cellular migration (1). Two types of sphingosine kinases, SPHK1 and SPHK2, are known to be expressed in human cells. The two enzymes share considerable amino acid sequence similarity, but differ in their N-terminal and central regions (2). The two proteins also differ in tissue distribution and some kinetic properties (2). Sphingosine kinases can be either cytosolic or membrane-associated. Differences in the distribution of SPHK1 and SPHK2 within cells may be the cause of differences that have been observed in the function of the two enzymes, particularly their roles in apoptosis (3).

1. Spiegel, S. (1999) J. Leukocyte Biol. 65:341.
2. Liu, H. et al. (2000) J. Biol. Chem. 275:19513.
3. Maceyka, M. et al. (2005) J. Biol. Chem. 280:37118.

Publications for Sphingosine Kinase 2/SPHK2 (5298-SK)(1)

Publication using 5298-SK

• N Turner, XY Lim, HD Toop, B Osborne, AE Brandon, EN Taylor, CE Fiveash, H Govindaraj, JD Teo, HP McEwen, TA Couttas, SM Butler, A Das, GM Kowalski, CR Bruce, KL Hoehn, T Fath, C Schmitz-Pe, GJ Cooney, MK Montgomery, JC Morris, AS Don A selective inhibitor of ceramide synthase 1 reveals a novel role in fat metabolism Nat Commun, 2018-08-21;9(1):3165. 2018-08-21 [PMID: 30131496] (Enzyme Assay, Human)

Bioinformatics

• Gene Symbol: SPHK2
• Uniprot:
  • Q9NRA0()