Recombinant Human SorLA His-tag Protein, CF

When Recombinant Human SorLA His-tag Protein (Catalog # 11083-LA) is coated at 0.250 μg/mL (100 μL/well), it binds to Recombinant Human PDGF-BB (220-BB). The ED50 for this binding is 2.00‑30.0 ng/mL.

2 μg/lane of Recombinant Human SorLA His-tag Protein (Catalog # 11083-LA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 270-345 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human SorLA His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human SorLA His-tag Protein is coated at 0.250 μg/mL (100 μL/well), it binds to Recombinant Human PDGF-BB (Catalog # 220-BB). The ED₅₀ for this binding is 2.00-30.0 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human SorLA protein
  Ser82-Asp2135, with a C-terminal 6-His tag
• Accession #: NP_003096.1
• N-terminal Sequence: Ser82
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 232 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 270-345 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 1.00 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SorLA His-tag Protein, CF

• C11orf32
• chromosome 11 open reading frame 32
• FLJ21930
• FLJ39258
• gp250
• Low-density lipoprotein receptor relative with 11 ligand-binding repeats
• LR11
• LR11LDLR relative with 11 ligand-binding repeats
• LRP9
• mosaic protein LR11
• SORL1
• SorLA
• SorLA-1SORLA
• sortilin-related receptor
• sortilin-related receptor, L(DLR class) A repeats containing
• sortilin-related receptor, L(DLR class) A repeats-containing
• Sorting protein-related receptor containing LDLR class A repeats

Background

SorLA (sortilin-related receptor, LDLR class A repeats-containing), also called LR11 or SORL1, is a 250 kDa type I transmembrane glycoprotein of the Sortilin family of Vps10p-domain receptors (1, 2). SorLA is found in both intracellular and surface membranes in the central nervous system, and mediates trafficking of proteins such as the amyloid precursor protein (APP) (3-5). The 2214 amino acid (aa) human SorLA precursor includes a 28 aa signal sequence and a 53 aa furin-cleaved propeptide. The mature SorLA contains a vacuolar protein sorting 10 protein (Vps10p) homology domain, beta -propeller and epidermal growth factor (EGF) domains, a cluster of 11 complement-type repeat domains, six fibronectin type III repeats, a single transmembrane domain (TM), and a cytoplasmic domain (CT) (1, 6-7). Human SorLA shares 93% and 92% aa sequence identity with mouse and rat SorLA, respectively. SorLA is mainly expressed in nervous system, but is also found in non-neuronal tissues (8). Upon truncation, SorLA is able to bind multiple ligands among which are RAP, apolipoprotein E, and lipoprotein lipase, and facilitate both their endocytosis and sorting (1). The expression, translocation, and ectodomain shedding were shown to be induced by its ligand head activator peptide (HA) (9). SorLA has also been shown to be important in Alzheimer's disease through multiple functions, but especially through its ability to bind Amyloid Precursor Protein (4). It is involved in TrkB receptor and GFRa1 trafficking and further enhancement of BDNF and GDNF functions respectively (10, 11).

1. Jacobsen, L. et al. (2001) J. Biol. Chem. 276:22788.
2. Wang, X. et al. (2011) Protein Expr. Purif. 77:224.
3. Small, S.A. and S. Gandy (2006) Neuron. 52:15.
4. Andersen, O.M. et al. (2005) Proc. Natl. Acad. Sci. U. S. A. 102:13461.
5. Willnow, T.E. et al. (2008) Nat. Rev. Neurosci. 9:899.
6. Herz J. (2001) Neuron 29:571.
7. Yamazaki H. et al. (1996) J. Biol. Chem. 271:24761.
8. Jacobsen L. et al. (1996) J. Biol. Chem. 271:31379.
9. Hampe, W. et al. (2000) J. Cell Sci. 113:4475.
10. Rohe, M. et al. (2013) PLoS One. 8:72164.
11. Glerup, S. et al. (2013) Cell Rep. 3:186.

Publications for SorLA (11083-LA)(1)

Publication using 11083-LA

• Chen, T;Liang, J;Liu, W;Luo, X;Zhou, F;Zhou, Y;Xing, S; A highly sensitive multicolor quantum dots-polylysine@FLISA platform for the simultaneous detection of serum PD-L1, SORL1, and GDF-15 levels Analytica chimica acta 2026-02-08 [PMID: 41545108] (Immunoassay Development, Human)

Bioinformatics

• Uniprot:
  • NP_003096.1(Human)