Recombinant Human SorCS3 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human SorCS3 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rhSorCS3 at 1 µg/mL (100 µL/well) can bind rh beta -NGF with an apparent K_D <75 nM.
• Source: Mouse myeloma cell line, NS0-derived human SorCS3 protein
  Ala134-Ser1125, with a C-terminal 6-His tag
• Accession #: Q9UPU3
• N-terminal Sequence: Ala134
• Protein/Peptide Type: Recombinant Proteins
• Gene: SORCS3
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 112.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 125-135 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SorCS3 Protein, CF

• 6330404A12Rik
• KIAA1059SORCS
• SorCS3
• sortilin-related VPS10 domain containing receptor 3
• VPS10 domain receptor protein SORCS 3 (SORCS3)
• VPS10 domain-containing receptor SorCS3

Background

SorCS3 (sortilin-related YPS10 domain containing receptor 3) is a type I transmembrane receptor that belongs to the mammalian Vps10p (vacuolar protein-sorting 10 protein) family of sorting receptors. This family includes sortilin, SorLA, and three SorCS proteins (1, 2). It is synthesized as a 1222 amino acid (aa) preproform with a 33 aa signal sequence and a 100 aa propeptide. After proteolytic processing at a furin-type consensus sequence, mature SorCS3 is generated that is 100 - 110 kDa in size and 1089 aa in length. It contains a 992 aa extracellular/lumenal domain (ECD), a 21 aa transmembrane segment and a 76 aa cytoplasmic domain. Human SorCS3 ECD shares 92%, 93%, 94% and 91% aa identity with mouse, rat, bovine and canine SorCS3 ECD, respectively. It also shares 70% and 46% aa identity with human SorCS1 and SorCS2 ECD, respectively. The ECD contains an imperfect leucine-rich repeat (LRR) and a Vps10p domain that binds both pro- and mature NGF (2, 3). The ECD may be shed constitutively, or upregulated when induced by phorbol esters (4). The metalloproteinase TACE/ADAM17 is known to cleave SorCS3 near the membrane (4). The shed ECD is able to bind PDGF-BB and the NGF propeptide (4, 5). The function of the SorCS3 propeptide is unknown. It does not block activity or influence protein processing in a manner analogous to the sortilin propeptide (3, 5). SorCS3 is predominantly expressed on the plasma membrane where it can be slowly internalized (3). Despite the presence of a sorting domain, there is no evidence for SorCS3-mediated intracellular trafficking activity (3). It is expressed in mouse embryonic and adult central nervous system in areas distinct from that of SorCS1 and SorCS2 (1). Neuronal activity upregulates SorCS3 expression in the mouse hippocampus (1).

1. Hermey, G. et al. (2004) J. Neurochem. 88:1470.
2. Hampe, W. et al. (2001) Hum. Genet. 108:529.
3. Westergaard, U. B. et al. (2005) FEBS Lett. 579:1172.
4. Hermey, G. et al. (2006) Biochem. J. 395:285.
5. Westergaard, U. et al. (2004) J. Biol. Chem. 279:50221.

Bioinformatics

• Gene Symbol: SORCS3
• Uniprot:
  • Q9UPU3()