Recombinant Human Serpin A6 Protein, CF

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When Cortisol-BSA Conjugate is immobilized at 5 μg/mL, 100μL/well, Recombinant Human Serpin A6 (Catalog # 9580‑PI) binds with an ED50 of1.5-7.5 μg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Serpin A6 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Cortisol-BSA Conjugate is immobilized at 5 μg/mL, 100 μL/well, Recombinant Human Serpin A6 binds with an ED50 of 1.5-7.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Serpin A6 protein
Met23-Val405, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Met23
Protein/Peptide Type
Recombinant Enzymes
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
50-70 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 1 mg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Serpin A6 Protein, CF

  • antitrypsin), member 6
  • CBG
  • CBGcorticosteroid-binding globulin
  • corticosteroid binding globulin
  • member 6
  • serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
  • Serpin A6
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
  • transcortin

Background

Corticosteroid-binding globulin (CBG), also known as Serpin A6, is an approximately 42 kDa member of the serpin superfamily of serine protease inhibitors. CBG belongs to the clade A subgroup of serpins which include inflammatory response molecules (1). Human CBG shares 57% sequence identity to mouse CBG. CBG is one of two serpins known to bind and transport hormones. CBG is responsible for binding ~90% of cortisol in a reversible manner to regulate levels under normal physiological states (2). However, cleavage of CBG's reactive site loop by proteases present during an inflammatory response leads to a targeted and immediate release of the anti-inflammatory steroid, cortisol, from CBG (3). Reduced cleavage of CBG is implicated in the inflammatory phenotype seen in obesity, metabolic syndrome, and rheumatoid arthritis (4, 5). Cleavage of CBG can be modulated by glycosylation (6). Naturally occurring mutations of CBG lead to abnormal levels or function (7, 8). Loss of functional CBG results in low cortisol levels in patients experiencing chronic pain, fatigue, hypotension and excess weight (8).
  1. Law, R. et al. (2006) Genome Biol. 7:216.
  2. Lewis, J. G. et al (2005) Clin. Chem. Acta. 359:189.
  3. Klieber M.A. et al. (2007) J. Biol. Chem. 282:29594.
  4. Nenke, M.A. et al. (2016) Horm. Metab. Res. 48:523.
  5. Nenke, M.A. et al. (2016) Clin. Endocrinol. 85:369.
  6. Sumer-Bayraktar, Z. et al. (2016) J. Biol. Chem 291:17727.
  7. Simard, M. et al. (2015) J. Clin. Endocrinol. Metab. 100:E129.
  8. Gagliardi, L. et al. (2010) Mol. Cell. Endocrinol. 316:24.

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