Recombinant Human Semaphorin 7A Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Semaphorin 7A Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin C1 (Catalog # 3887-PC) is coated at 5 μg/mL, Recombinant Human Semaphorin 7A Fc Chimera binds with an apparent K_d <5 nM.
• Source: Mouse myeloma cell line, NS0-derived human Semaphorin 7A protein
  

  Human Semaphorin 7A (His47-Ala648) Accession # O75326	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: O75326
• N-terminal Sequence: His47
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: SEMA7A
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 94.8 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100-105 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Semaphorin 7A Fc Chimera Protein, CF

• CD108 antigen
• CD108
• CD108MGC126696
• CDw108
• H-SEMA-K1
• H-Sema-L
• JMH blood group antigen
• JMH
• John-Milton-Hargen human blood group Ag
• MGC126692
• sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A (JMH blood group)
• sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A
• sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A
• Sema K1
• Sema L
• Sema7A
• SEMAK1
• SEMAL
• SEMALJohn Milton Hagen blood group H-Sema K1
• Semaphorin 7A
• semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)
• semaphorin K1
• semaphorin L
• semaphorin-7A
• semaphorin-K1
• semaphorin-L

Background

Semaphorin 7A (Sema7A, designated CD108, previously Sema K1 or Sema L), is an ~80 kDa membrane-anchored glycoprotein that is a member of the Semaphorin family of axon guidance molecules (1 ‑ 4). On human erythrocytes, it is the John Milton Hagen (JMH) blood group antigen (4). Sema7A is the only known Class 7 or glycophosphatidylinositol (GPI)-linked semaphorin; its expression is concentrated in the brain, spleen and thymus (1 ‑ 5). Human Sema7A cDNA encodes a 44 amino acid (aa) signal sequence, a 604 aa extracellular domain (ECD) including Sema and C2‑type Ig-like domains, and an 18 aa propeptide/GPI membrane anchor signal sequence. Mature human Sema7A shares 89%, 89%, 88%, 86% and 90% aa identity with mouse, rat, canine, bovine and equine Sema7A, respectively. The Sema7A sema domain contains an RGD integrin interaction motif (4). Although it binds plexin-C1 in vitro and may be coexpressed with it, many of its activities depend on interaction with beta 1 integrins such as alpha 1 beta 1 (6 ‑ 10). Sema7A signaling through the two receptors may cause opposing effects (8). Sema7A is an immune semaphorin, with expression and activity on CD4⁺CD8⁺ thymocytes, activated T cells, macrophages and microglia (2, 9 ‑ 12). T cell Sema7A interacts with monocytic cells, stimulating their chemotaxis, production of pro-inflammatory cytokines, and dendritic differentiation (5, 6). However, on the T cells themselves, Sema7A downregulates TCR signaling by promoting TCR internalization, modulating T cell responses (9). In lung macrophages, Sema7A is induced by TGF-beta and participates in TGF-beta -induced lung fibrosis (12). Sema7A is also expressed on pre-osteoblasts and osteoclasts, where it promotes migration and fusion, respectively; on keratinocytes, where it promotes melanocyte spreading and dendricity; and on some neurons, for example, promoting axon outgrowth in the developing olfactory tract (8, 10, 13). 

1. Yazdani, U. and J.R. Terman (2006) Genome Biol. 7:211.
2. Kikutani, H. et al. (2007) Adv. Immunol. 93:121.
3. Sato, Y. and Takahashi H. (1998) Biochim. Biophys. Acta. 1443:419.
4. Yamada, A. et al. (1999) J. Immunol. 162:4094.
5. Holmes, S. et al. (2002) Scand. J. Immunol. 56:270.
6. Suzuki, K. et al. (2007) Nature 446:680.
7. Pasterkamp, R.J. et al. (2007) BMC Dev. Biol. 7:98.
8. Scott, G.A. et al. (2007) J. Invest. Dermatol. 128:151.
9. Czopik, A.K. et al. (2006) Immunity 24:591.
10. Pasterkamp, R.J. et al. (2003) Nature 424:398.
11. Mine, T. et al. (2000) Tissue Antigens 55:429.
12. Kang, H.-R. et al. (2007) J. Exp. Med. 204:1083.
13. Delorme, G. et al. (2005) Biol. Cell 97:589.

Publications for Semaphorin 7A (2068-S7)(5)

Publications using 2068-S7

• Mohanakrishnan, V;Sivaraj, KK;Jeong, HW;Bovay, E;Dharmalingam, B;Bixel, MG;Dinh, VV;Petkova, M;Paredes Ugarte, I;Kuo, YT;Gurusamy, M;Raftrey, B;Chu, NTL;Das, S;Rios Coronado, PE;Stehling, M;Sävendahl, L;Chagin, AS;Mäkinen, T;Red-Horse, K;Adams, RH; Specialized post-arterial capillaries facilitate adult bone remodelling Nature cell biology 2024-11-11 [PMID: 39528700] (Bioassay, Human)
• Huang, J;Zhao, C;Zhang, S; Semaphorin 7A promotes endothelial permeability and inflammation via plexin C1 and integrin ?1 in Kawasaki disease BMC pediatrics 2024-04-27 [PMID: 38678170] (Bioassay, Human)
• I Manini, ME Ruaro, R Sgarra, A Bartolini, F Caponnetto, T Ius, M Skrap, C Di Loreto, AP Beltrami, G Manfiolett, D Cesselli Semaphorin-7A on Exosomes: A Promigratory Signal in the Glioma Microenvironment Cancers (Basel), 2019-05-30;11(6):. 2019-05-30 [PMID: 31151295] (Bioassay, Human)
• Beta 1-integrin ligation and TLR ligation enhance GM-CSF-induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties Sci Rep, 2016-11-29;6(0):37914. 2016-11-29 [PMID: 27897208] (Bioassay, Mouse)
• Roth J, Kohler D, Schneider M, Granja T, Rosenberger P Semaphorin 7A Aggravates Pulmonary Inflammation during Lung Injury. PLoS ONE, 2016-01-11;11(1):e0146930. 2016-01-11 [PMID: 26752048] (Bioassay, Human)

Bioinformatics

• Gene Symbol: SEMA7A
• Uniprot:
  • O75326()