Recombinant Human Semaphorin 3E Protein, CF


There are currently no images for Semaphorin 3E (3239-S3B).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity HuSpecies Glossary
Applications Binding Activity, Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human Semaphorin 3E Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. Moriya, J. et al. (2010) Circ. Res. 106:391. The ED50 for this effect is 0.04-0.24 μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin D1 (Catalog # 4160-PD) is coated at 5 μg/mL, Recombinant Human Semaphorin 3E binds with an apparent Kd <0.4 nM.

Mouse myeloma cell line, NS0-derived human Semaphorin 3E protein
His24-Ser775 (Arg557Ala and Arg560Ala), with an N-terminal 10-His tag
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Binding Activity2
  • Bioactivity
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
85-100 kDa, 66 kDa and 25 kDa, reducing conditions
Read Publications using
3239-S3B in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and Tween® 20 with Trehalose.
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Semaphorin 3E Protein, CF

  • (semaphorin) 3E
  • coll-5
  • sema domain, immunoglobulin domain (Ig), short basic domain, secreted
  • Sema3E
  • SEMAHM-SemaK
  • Semaphorin 3E
  • semaphorin-3E


Semaphorin 3E (Sema3E), previously known as SemaH, is one of six Class 3 (secreted) semaphorins which function in axon guidance and/or vascular tip cell guidance during development (1). Sema3E contains a seven-blade beta -propeller sema domain, a cysteine-knot PSI domain, an Ig-like domain, and a basic region. Dimerization and cleavage within the basic region are required for the repulsing activity of class 3 semaphorins (2). Sema3E can also be cleaved at a furin consensus sequence C-terminal to the sema domain, resulting in a 61 kDa form that does not dimerize and is highly expressed in tumor cell lines with metastatic potential (3, 4). Mature human Sema3E shares 90% aa sequence identity with mouse and rat Sema3E. Alternative splicing generates a short isoform that lacks the signal peptide and the N-terminal 35 residues of the mature protein. Sema3E signaling is transduced by Plexin D1 which may also be associated with Neuropilin 1 and/or VEGF R2 (2, 5, 6). Its interaction with Plexin D1 inhibits axon migration in the neocortex and forebrain (6, 7), although it can attract axons that express both Plexin D1 and Neuropilin 1 (6). Sema3E promotes axonal growth (5), the development of glutamatergic synaptic specificity (8, 9), and the development of GnRH producing neurons (10). Genetic disruption of either Sema3E or Plexin D1 in mouse causes excessive and disorganized vascular growth and branching, indicating the importance of this ligand-receptor pair for vascular guidance (11, 12). In addition, Sema3E is up-regulated by inflammatory macrophages and damaged hepatocytes (13-15). It inhibits smooth muscle cell proliferation and migration in the asthmatic airway (16), promotes hepatic stellate cell activation and wound healing (15), and regulates the migration of developing thymocytes (17).
  1. Oh, W.J. and C. Gu (2013) Semin. Cell Dev. Biol. 24:156.
  2. Adams, R. H. et al. (1997) EMBO J. 16:6077.
  3. Christensen, C. et al. (2005) Cancer Res. 65:6167.
  4. Casazza, A. et al. (2010) J. Clin. Invest. 120:2684.
  5. Bellon, A. et al. (2010) Neuron 66:205.
  6. Chauvet, S. et al. (2007) Neuron 56:807.
  7. Bribian, A. et al. (2014) Nat. Commun. 5:4265.
  8. Ding, J.B. et al. (2011) Nat. Neurosci. 15:215.
  9. Pecho-Vrieseling, E. et al. (2009) Nature 459:842.
  10. Cariboni, A. et al. (2015) J. Clin. Invest. 125:2413.
  11. Gu, C. et al. (2005) Science 307:265.
  12. Gitler, A. D. et al. (2004) Developmental Cell 7:107.
  13. Wanschel, A. et al. (2013) Arterioscler. Thromb. Vasc. Biol. 33:886.
  14. Shimizu, I. et al. (2013) Cell Metab. 18:491.
  15. Yagai, T. et al. (2014) Am. J. Pathol. 184:2250.
  16. Movassagh, H. et al. (2014) J. Allergy Clin. Immunol. 133:560.
  17. Choi, Y.I. et al. (2014) Proc. Natl. Acad. Sci. USA 111:379.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut, WB
Species: Hu
Applications: BA, BA
Species: Mu, Rt
Applications: Block, CyTOF-ready, Flow, IHC, WB
Species: Hu, Mu, Rt
Applications: Block, CyTOF-ready, Flow, IHC, Simple Western, WB
Species: Bv, Ca, Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
Species: Hu
Applications: ELISA
Species: Hu
Applications: IHC, IHC-P
Species: Mu
Applications: BA
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB
Species: Hu, Mu
Applications: ICC, IHC, WB
Species: Mu
Applications: CyTOF-ready, Flow, IHC, Neut, WB
Species: Hu
Applications: BA
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Species: Mu, Rt
Applications: IHC, WB
Species: Bv, Hu, Mu
Applications: IHC, IHC-P, WB
Species: Hu
Applications: IHC, IHC-P
Species: Hu
Applications: EnzAct
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, WB
Species: Hu
Applications: Binding Activity, Bioactivity

Publications for Semaphorin 3E (3239-S3B)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Bioassay, Migration Bioassay.

Filter By Application
Migration Bioassay
All Applications
Filter By Species
All Species

Reviews for Semaphorin 3E (3239-S3B) (0)

There are no reviews for Semaphorin 3E (3239-S3B). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Semaphorin 3E (3239-S3B) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Semaphorin 3E Products

Bioinformatics Tool for Semaphorin 3E (3239-S3B)

Discover related pathways, diseases and genes to Semaphorin 3E (3239-S3B). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Semaphorin 3E (3239-S3B)

Discover more about diseases related to Semaphorin 3E (3239-S3B).

Pathways for Semaphorin 3E (3239-S3B)

View related products by pathway.

PTMs for Semaphorin 3E (3239-S3B)

Learn more about PTMs related to Semaphorin 3E (3239-S3B).

Blogs on Semaphorin 3E

There are no specific blogs for Semaphorin 3E, but you can read our latest blog posts.

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human Semaphorin 3E Protein, CF and receive a gift card or discount.


Gene Symbol SEMA3E