Recombinant Human ROR1 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human ROR1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Measured by its ability to bind biotinylated recombinant human/mouse Wnt-5a in a functional ELISA. The ED 50 for this effect is 8-48 µg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human ROR1 protein

  Human ROR1 (Ala24-Glu403) Accession # Q01973-1

  IEGRMD

  Human IgG 1 (Pro100-Lys330)

  N-terminus

  C-terminus

• Accession #: Q01973-1
• N-terminal Sequence: Ala24
• Structure / Form: Disulfide -linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 84-97 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ROR1 Fc Chimera Protein, CF

• EC 2.7.10.1
• MGC99659
• neurotrophic tyrosine kinase receptor-related 1
• Neurotrophic tyrosine kinase, receptor-related 1
• NTRKR1
• NTRKR1dJ537F10.1
• receptor tyrosine kinase-like orphan receptor 1
• ROR1
• tyrosine-protein kinase transmembrane receptor ROR1

Background

ROR1 (Receptor tyrosine kinase-like orphan receptor 1), also known as neurotrophic tyrosine kinase receptor-related 1 (NTRKR1), is a member of the ROR family within receptor tyrosine kinases (RTK) superfamily. Two ROR family members (ROR1 and ROR2) have been identified and are characterized by the intracellular tyrosine kinase domains, highly related to those of the Trk-family receptor tyrosine kinases, and by the extracellular Frizzled-like cysteine-rich domains and kringle domains, which are common to receptors of the Wnt family members (1, 2). Human ROR1 is a type I transmembrane protein with 937 amino acids (aa) in length. It contains a 29 aa signal sequence, a 377 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 510 aa cytoplasmic region. Human ROR1 shares 97% and 58% aa sequence identity with mouse ROR1 and human ROR2, respectively (2, 3). ROR1 has been shown to play crucial roles in developmental morphogenesis by acting as receptors or co-receptors to mediate Wnt5a-induced signaling (4). The bioactivity of ROR1 is measured by its ability to bind biotinylated recombinant mouse Wnt-5a in a functional ELISA.

1. Aghebati-Maleki, L. et al. (2017) Biomed. Pharmacother. 88:814.
2. Borcherding N. et al. (2014) Protein Cell. 5:496.
3. Masiakowski P. and Carroll R.D. (1992) J. Biol. Chem. 267:26181.
4. Fukuda T. et al. (2008) Proc. Natl. Acad. Sci. U.S.A.  105:3047.

Bioinformatics

• Uniprot:
  • Q01973-1()