Recombinant Human ROR1 Fc Chimera Avi-tag Protein, CF

When Human ROR1 Antibody (Catalog # MAB20001) is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human ROR1 Fc Chimera Avi-tag (Catalog # AVI9490) binds with an ED50 of 0.6‑3.6 ng/mL.

2 μg/lane of Biotinylated Recombinant Human ROR1 Fc Chimera Avi-tag (Catalog # AVI9490) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-105 kDa and 170-210 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human ROR1 Fc Chimera Avi-tag Protein, CF Summary

• Additional Information: Biotinylated
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Human ROR1 Antibody (Catalog # MAB20001) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ROR1 Fc Chimera Avi-tag (Catalog # AVI9490) binds with an ED₅₀ of 0.6-3.6 ng/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human/Mouse Wnt-5a Recombinant Human/Mouse Wnt‑5a (Catalog # 645-WN) is immobilized at 4 µg/mL (100 µL/well), Biotinylated Recombinant Human ROR1 Fc Chimera Avi-tag (Catalog # AVI9490) binds with an ED₅₀ of 5-40 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human ROR1 protein
  

  Human ROR1 (Ala24-Glu403) Accession # Q01973	IEGRMD	Human IgG1 (Pro100-Lys330)	GG	Avi-tag

• Accession #: Q01973
• N-terminal Sequence:

  Ala24

• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85-105 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ROR1 Fc Chimera Avi-tag Protein, CF

• EC 2.7.10.1
• MGC99659
• neurotrophic tyrosine kinase receptor-related 1
• Neurotrophic tyrosine kinase, receptor-related 1
• NTRKR1
• NTRKR1dJ537F10.1
• receptor tyrosine kinase-like orphan receptor 1
• ROR1
• tyrosine-protein kinase transmembrane receptor ROR1

Background

ROR1 (Receptor tyrosine kinase-like orphan receptor 1), also known as neurotrophic tyrosine kinase receptor-related 1 (NTRKR1),  is a member of  the ROR family within receptor tyrosine kinases (RTK) superfamily. Two ROR family members (ROR1 and ROR2) have been identified and are characterized by the intracellular tyrosine kinase domains, highly related to those of the Trk-family receptor tyrosine kinases, and by the extracellular Frizzled-like cysteine-rich domains and kringle domains, which are common to receptors of the Wnt family members (1, 2). Human ROR1 is a type I transmembrane protein with 937 amino acids (aa) in length. It contains a 29 aa signal sequence, a 377 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 510 aa cytoplasmic region. Human ROR1 shares 97% and 58% aa sequence identity with mouse ROR1 and human ROR2, respectively (2, 3). ROR1 has been shown to play crucial roles in developmental morphogenesis by acting as receptors or co-receptors to mediate Wnt5a-induced signaling (4). The bioactivity of ROR1 is measured by its ability to bind biotinylated recombinant mouse Wnt-5a in a functional ELISA.

1. Aghebati-Maleki, L. et al. (2017) Biomed. Pharmacother. 88:814.
2. Borcherding N. et al. (2014) Protein Cell. 5:496.
3. Masiakowski P. and Carroll R.D. (1992) J. Biol. Chem. 267:26181.
4. Fukuda T. et al. (2008) Proc. Natl. Acad. Sci. U.S.A.  105:3047.

Bioinformatics

• Uniprot:
  • Q01973()