Recombinant Human ROBO2 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human ROBO2 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Biotinylated recombinant mouse Slit-3 immobilized on a streptavidin-coated plate at 1 µg/mL can bind Recombinant Human ROBO2 Fc Chimera with an apparent K d <10 nM.
• Source: Mouse myeloma cell line, NS0-derived human ROBO2 protein

  Human ROBO2 (Ser22-Pro863) Accession # NP_001276969

  IEGRMD

  Human IgG 1 (Pro100-Lys330)

  N-terminus

  C-terminus

• Accession #: NP_001276969
• N-terminal Sequence: Ser22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: ROBO2
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 120 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 146-160 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ROBO2 Fc Chimera Protein, CF

• Drosophila) homolog 2
• ROBO2
• roundabout, axon guidance receptor, homolog 2 (Drosophila)
• SAX3

Background

Human ROBO2 is a 175 kDa member of the ROBO family of guidance molecules (1-3). The term ROBO derives from round-about, a description of the circuitous pathway axons take in the absence of a functional ROBO gene (3, 4). Human ROBO2 is a type I transmembrane (TM) glycoptotein that is synthesized as a 1378 amino acid (aa) precursor. It contains a 21 aa signal sequence, an 838 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 498 aa cytoplasmic region (5, 6). The ECD contains five C2-type Ig-like domains (aa 31-504) and three fibronectin (FN) type III domains (aa 522-826). The cytoplasmic region contains multiple 15-20 aa long CC (conserved cytoplasmic) motifs (C0-C2) that are found in ROBO-1 (7, 8). Human ROBO2 has at least two potential isoforms. One isoform shows a cytoplasmic truncation of the C-terminus (aa 1186-1378) (9). A second isoform is a 570 aa soluble form that shows a deletion of the first two and one-half C2-type Ig-like domains (aa 22 - 285) and terminates after the third fibronectin domain with a unique 20 aa sequence (10). Based on mouse and rat ROBO2 GenBank sequences, considerably more alternate splice forms may occur. Human ROBO2 ECD is 98% aa identical to the ECD in mouse and canine ROBO2. ROBO2 would appear to play a number of roles in cell adhesion. In the neural tube, it is the receptor for a chemorepulsant. Axons that cross the midline are directed, presumably by ROBO2/SLIT chemorepulsion, to lateral positions in the contralateral spinal cord (6, 11, 12). In addition, ROBO2 is both permissive for neurite outgrowth (via ROBO1-ROBO2 interaction) and inhibitory for neurite outgrowth (via ROBO2-SLIT interaction) (13, 14).

1. Rajagopalan, S. et al. (2000) Neuron 28:767.
2. Guthrie, S. (2004) Curr. Biol. 14:R632.
3. Guthrie, S. (2001) Curr. Biol. 11:R300.
4. Seeger, M. et al. (1993) Neuron 10:409.
5. Kidd, T. et al. (1998) Cell 92:205.
6. Nguyen Ba-Charvet, K.T. et al. (2001) J. Neurosci. 21:4281.
7. Bashaw, G.J. et al. (2000) Cell 101:703.
8. Jen, J.C. et al. (2004) Science 304:1509.
9. GenBank Accession # Q9KCK4.
10. Isogai, T. et al. (2002) GenBank Accession # AK074780.
11. Mambetisaeva, E.T. et al. (2005) Dev. Dyn. 233:41.
12. Long, H. et al. (2004) Neuron 42:213.
13. Hivert, B. et al. (2002) Mol. Cell. Neurosci. 21:534.
14. Lin, L. et al. (2005) Mol. Cell. Neurosci. 28:547.

Publications for ROBO2 (3147-RB)(3)

Publications using 3147-RB

• AN Gonçalves, J Correia-Pi, C Nogueira-S ROBO2 signaling in lung development regulates SOX2/SOX9 balance, branching morphogenesis and is dysregulated in nitrofen-induced congenital diaphragmatic hernia Respir Res, 2020-11-18;21(1):302. 2020-11-18 [PMID: 33208157] (Cell Culture, Rat)
• Greaves E, Collins F, Esnal-Zufiaurre A, Giakoumelou S, Horne A, Saunders P Estrogen receptor (ER) agonists differentially regulate neuroangiogenesis in peritoneal endometriosis via the repellent factor SLIT3. Endocrinology, 2014-07-22;155(10):4015-26. 2014-07-22 [PMID: 25051436] (Bioassay, Human)
• Ricano-Cornejo I, Altick AL, Garcia-Pena CM, Nural HF, Echevarria D, Miquelajauregui A, Mastick GS, Varela-Echavarria A Slit-Robo signals regulate pioneer axon pathfinding of the tract of the postoptic commissure in the mammalian forebrain. J. Neurosci. Res., 2011-06-17;89(10):1531-41. 2011-06-17 [PMID: 21688288] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: ROBO2
• Uniprot:
  • NP_001276969()