Recombinant Human RNF4 Protein, CF

Product Discontinued

Recombinant Human RNF4 Protein, CF Summary

• Details of Functionality: Recombinant Human RNF4 is a Ubiquitin ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human RNF4 concentration of 100-500 nM.
• Source: E. coli-derived human RNF4 protein
• Accession #: P78317
• Protein/Peptide Type: Recombinant Enzymes
• Gene: RNF4
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 5 mM DTT
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human RNF4 Protein, CF

• E3 ubiquitin ligase RNF4
• EC 6.3.2.-
• Protein SNURF
• RES4-26
• ring finger protein 4SNURFSLX5
• RNF4
• SLX5
• Small nuclear ring finger protein
• SNURF

Background

RNF4 (small nuclear ring finger protein, SNURF) is a RING-finger ubiquitin E3 ligase that ubiquitinates and mediates the proteasomal destruction of targets such as PML, PEA3, CENP1, and PARP1. In addition to the RING domain, RNF4 contains four SUMO-interacting motifs (SIMs) that function to recruit this ligase to poly-sumoylated substrates. RNF4 will autoubiquitinate in vitro, and will also ubiquitinate poly-SUMO chains.

1. Geoffroy, M-C et al. (2010) Mol. Bio. Cell 21: 4227
2. Tathum, M.H. et al. (2008) Nat. Cell Bio. 10: 538

Publications for RNF4 (E3-210)(2)

Publications using E3-210

• Y Sun, J Chen, SN Huang, YP Su, W Wang, K Agama, S Saha, LM Jenkins, JM Pascal, Y Pommier PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation Nature Communications, 2021-08-18;12(1):5010. 2021-08-18 [PMID: 34408146] (Bioassay, Human)
• CW Davies, SE Vidal, L Phu, J Sudhamsu, TB Hinkle, S Chan Rosen, FR Schumacher, YJ Zeng, C Schwerdtfe, AS Peterson, JR Lill, CM Rose, AS Shaw, IE Wertz, DS Kirkpatric, JT Koerber Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W Nature Communications, 2021-07-29;12(1):4608. 2021-07-29 [PMID: 34326324] (Bioassay, Human)

Bioinformatics

• Gene Symbol: RNF4
• Uniprot:
  • P78317(Human)