Recombinant Human RANK/TNFRSF11A Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human RANK/TNFRSF11A Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit TRANCE-induced osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 0.005‑0.035 µg/mL in the presence of 2 ng/mL of rmTRANCE (Catalog # 462-TEC).
Source
Mouse myeloma cell line, NS0-derived human RANK/TNFRSF11A protein
Met Human RANK
(Gln29-Gly213)
Accession # Q9Y6Q6.1
IEGRDMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TNFRSF11A
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
48 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60 kDa, reducing conditions
Publications
Read Publications using
683-RK in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human RANK/TNFRSF11A Fc Chimera Protein, CF

  • CD265 antigen
  • CD265
  • FEO
  • loss of heterozygosity, 18, chromosomal region 1
  • ODFR
  • ODFROSTS
  • OFE
  • OPTB7
  • Osteoclast differentiation factor receptor
  • PDB2
  • RANK
  • RANKLOH18CR1
  • Receptor activator of NF-KB
  • receptor activator of nuclear factor-kappa B
  • TNFRSF11A
  • TRANCE R
  • TRANCER
  • tumor necrosis factor receptor superfamily member 11A
  • tumor necrosis factor receptor superfamily, member 11a, activator of NFKB
  • tumor necrosis factor receptor superfamily, member 11a, NFKB activator

Background

RANK (receptor activator of NF-kappa B, also known as TRANCE receptor, osteoclast differentiation factor receptor [ODFR]) and TNFRSF11A is a member of the tumor necrosis factor receptor family. The full length human RANK cDNA encodes a type I transmembrane protein of 616 amino acids with a predicted 184 amino acid extracellular domain and a 383 amino acid cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains. RANK is widely expressed with highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is expressed in dendritic cells. In activated human peripheral blood T lymphocytes, RANK expression is induced by IL-4 and TGF-beta. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand [RANKL], osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Soluble RANK is able to block TRANCE induced biological activity.

  1. Anderson, D.M. et al. (1997) Nature 390:175.
  2. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.

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683-RK
Species: Hu
Applications: Bioactivity

Publications for RANK/TNFRSF11A (683-RK)(6)

We have publications tested in 3 confirmed species: Human, Hamster, N/A.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(6)
All Applications
Filter By Species
Human
(4)
Hamster
(1)
N/A
(1)
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Showing Publications 1 - 6 of 6.
Publications using 683-RK Applications Species
Justin R Savage A Modified Glycosaminoglycan, GM-0111, Inhibits Molecular Signaling Involved in Periodontitis PLoS ONE, 2016;11(6):e0157310. 2016 [PMID: 27308827] (Bioassay, N/A) Bioassay N/A
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Colucci S, Brunetti G, Mori G, Oranger A, Centonze M, Mori C, Cantatore FP, Tamma R, Rizzi R, Liso V, Zallone A, Grano M Soluble decoy receptor 3 modulates the survival and formation of osteoclasts from multiple myeloma bone disease patients. Leukemia, 2009;23(11):2139-46. 2009 [PMID: 19587706] (Bioassay, Human) Bioassay Human
Colucci S, Brunetti G, Cantatore FP, Oranger A, Mori G, Quarta L, Cirulli N, Mancini L, Corrado A, Grassi FR, Grano M Lymphocytes and synovial fluid fibroblasts support osteoclastogenesis through RANKL, TNFalpha, and IL-7 in an in vitro model derived from human psoriatic arthritis. J. Pathol., 2007;212(1):47-55. 2007 [PMID: 17370327] (Bioassay, Human) Bioassay Human
Edwards JR, Sun SG, Locklin R, Shipman CM, Adamopoulos IE, Athanasou NA, Sabokbar A LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis. Arthritis Rheum., 2006;54(5):1451-62. 2006 [PMID: 16649193] (Bioassay, Human) Bioassay Human
Okuma K, Dalton KP, Buonocore L, Ramsburg E, Rose JK Development of a novel surrogate virus for human T-cell leukemia virus type 1: inhibition of infection by osteoprotegerin. J. Virol., 2003;77(15):8562-9. 2003 [PMID: 12857926] (Bioassay, Hamster) Bioassay Hamster

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Bioinformatics

Gene Symbol TNFRSF11A
Entrez
Uniprot