Recombinant Human PTH Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human PTH Protein, CF Summary

• Details of Functionality: Measured by its ability to induce cAMP accumulation in MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 10-60 ng/mL.
• Source: E. coli-derived human PTH protein
  Ser32-Gln115
• Accession #: P01270
• N-terminal Sequence: Ser32
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: PTH
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 9.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 9 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PTH Protein, CF

• Parathormone
• Parathyrin
• parathyroid hormone 1
• parathyroid hormone
• PTH
• PTH1

Background

PTH (parathyroid hormone) is a critical hormone in the regulation of Ca⁺⁺ homeostasis (1). The human PTH cDNA encodes 115 amino acids (aa) including a 25 aa signal sequence, a 6 aa propeptide, and an 84 aa mature hormone. Mature human PTH shares 70%, 73%, 88%, 87%, 86%, 86% and 85% aa identity with mouse, rat, canine, equine, bovine, porcine and feline PTH, respectively. Multiple N‑terminal peptides and C-terminal peptides derived from PTH occur naturally in the circulation (1). PTH aa 32-66, called PTH (1-34) since it represents the first 34 aa of the mature hormone, reproduces all the activity of the full length mature hormone and has been used therapeutically for treatment of osteoporosis (1-3). C-terminal peptides mainly oppose the activities of PTH (1-34) and are increased in renal failure (1-3). PTH expression is mainly restricted to the parathyroid gland, with minor amounts in the thymus (4). PTH secretion is enhanced by low Ca⁺⁺ concentrations and inhibited by FGF-23 (1, 5). In normal human plasma, PTH correlates negatively with active Vitamin D and positively with ionized calcium (6). Human and other mammalian PTH will bind and stimulate human or rat PTH1R, activating adenylate cyclase and increasing cAMP production (2, 7). PTH promotes secretion of TRANCE/RANKL and periostin through PTH1R binding on osteoblasts and/or bone marrow stromal cells (8-10). TRANCE/RANKL induces differentiation of osteoclasts, which in turn promote release of Ca⁺⁺ from bone (1, 8, 9). PTH1R on osteocytes, however, allows PTH to promote bone formation and IGF-1 production (11, 12). In renal epithelium, PTH promotes conversion of Vitamin D to its active form, lowers Ca⁺⁺ excretion and increases phosphate excretion (1, 2, 9). PTH also increases hematopoietic stem cell proliferation and mobilization and induces arterial vasodilation by regulating Ca⁺⁺ influx in PTH1R-expressing arterial smooth muscle (8, 13).

1. Potts, J.T. (2005) J. Endocrinol. 187:311.
2. Usatii, M. et al. (2007) Kidney Int. 72:1330.
3. Scillitani, A. et al. (2011) J. Clin. Invest. 34:23.
4. Liu, Z. et al. (2010) PLoS Genet. 6:e1001251.
5. Ben-Dov, I.Z. et al. (2007) J. Clin. Invest. 117:4003.
6. Carnevale, V. et al. (2010) Bone 47:626.
7. Schipani, E. et al. (1993) Endocrinology 132:2157.
8. Jacome-Galarza, C.E. et al. (2011) J. Bone Miner. Res. 26:1207.
9. Lupp, A. et al. (2010) Eur. J. Endocrinol. 162:979.
10. Fortunati, D. et al. (2010) Matrix Biol. 29:594.
11. O’Brien, C.A. et al. (2008) PLoS ONE 3:e2942.
12. Lombardi, G. et al. (2010) J. Endocrinol. Invest. 33:22.
13. Zaruba, M.M. et al. (2008) Cardiovasc. Res. 77:722.

Publications for PTH (7665-PT)(1)

Publication using 7665-PT

• Gadi , Jogeswar, Jung , Seung-Hy, Lee , Min-Jung, Jami , Ajita, Ruthala , Kalyani, Kim , Kyoung-M, Cho , Nam-Hoon, Jung , Han-Sung, Kim , Cheol-He, Lim , Sung-Kil The transcription factor protein Sox11 enhances early osteoblast differentiation by facilitating proliferation and the survival of mesenchymal and osteoblast progenitors. J Biol Chem, 2013-07-25;288(35):25400-13. 2013-07-25 [PMID: 23888050] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: PTH
• Uniprot:
  • P01270()